Live imaging of cellular dynamics using a multi-imaging vector in single cells

2014 ◽  
Vol 50 (73) ◽  
pp. 10734-10736 ◽  
Author(s):  
Kyoungsook Park ◽  
Jinyoung Jeong ◽  
Bong Hyun Chung
Keyword(s):  
Real Time ◽  
Single Cells ◽  
Live Imaging ◽  
Vector System ◽  
Real Time Monitoring ◽  
Cellular Dynamics

A multi-imaging vector system based on 2A peptides is developed for real-time monitoring of cellular dynamics.

scholarly journals Construction of micro-nano electrochemical sensing interfaces for real-time monitoring of single cells

2020 ◽  
Vol 51 (3) ◽  
pp. 359-373
Author(s):  
Yu-Ting Qi ◽  
Fu-Li Zhang ◽  
Si-Yu Tian ◽  
Xiao-Ke Yang ◽  
Yan-Ling Liu ◽  
...  
Keyword(s):  
Real Time ◽  
Single Cells ◽  
Electrochemical Sensing ◽  
Real Time Monitoring

Real-time monitoring of H2O2 release from single cells using nanoporous gold microelectrodes decorated with platinum nanoparticles

The Analyst ◽  
2015 ◽  
Vol 140 (11) ◽  
pp. 3753-3758 ◽  
Author(s):  
Chong Xiao ◽  
Yan-Ling Liu ◽  
Jia-Quan Xu ◽  
Song-Wei Lv ◽  
Shan Guo ◽  
...  
Keyword(s):  
Real Time ◽  
Platinum Nanoparticles ◽  
Single Cells ◽  
Nanoporous Gold ◽  
Real Time Monitoring

We present a self-supported nanoporous gold/PtNPs microelectrode with the sub-nanomolar sensitivity to detect H2O2 release from single cells.

scholarly journals Multi-flow channel bioreactor enables real-time monitoring of cellular dynamics in 3D engineered tissue

2019 ◽  
Vol 2 (1) ◽  
Author(s):  
Barak Zohar ◽  
Yaron Blinder ◽  
Mark Epshtein ◽  
Ariel A. Szklanny ◽  
Ben Kaplan ◽  
...  
Keyword(s):  
Real Time ◽  
Flow Channel ◽  
Real Time Monitoring ◽  
Cellular Dynamics ◽  
Engineered Tissue

scholarly journals A gapmer aptamer nanobiosensor for real-time monitoring of transcription and translation in single cells

Biomaterials ◽  
2018 ◽  
Vol 156 ◽  
pp. 56-64 ◽  
Author(s):  
Shue Wang ◽  
Yuan Xiao ◽  
Donna D. Zhang ◽  
Pak Kin Wong
Keyword(s):  
Real Time ◽  
Single Cells ◽  
Real Time Monitoring

CRISPR-mediated live imaging of genome editing and transcription

Science ◽  
2019 ◽  
Vol 365 (6459) ◽  
pp. 1301-1305 ◽  
Author(s):  
Haifeng Wang ◽  
Muneaki Nakamura ◽  
Timothy R. Abbott ◽  
Dehua Zhao ◽  
Kaiwen Luo ◽  
...  
Keyword(s):  
Real Time ◽  
Genome Editing ◽  
Single Cells ◽  
Cell Types ◽  
Live Imaging ◽  
Dna Double Strand Breaks ◽  
Chromosomal Disorders ◽  
Dna And Rna ◽  
Multiple Loci ◽  
Patau Syndrome

We report a robust, versatile approach called CRISPR live-cell fluorescent in situ hybridization (LiveFISH) using fluorescent oligonucleotides for genome tracking in a broad range of cell types, including primary cells. An intrinsic stability switch of CRISPR guide RNAs enables LiveFISH to accurately detect chromosomal disorders such as Patau syndrome in prenatal amniotic fluid cells and track multiple loci in human T lymphocytes. In addition, LiveFISH tracks the real-time movement of DNA double-strand breaks induced by CRISPR-Cas9–mediated editing and consequent chromosome translocations. Finally, by combining Cas9 and Cas13 systems, LiveFISH allows for simultaneous visualization of genomic DNA and RNA transcripts in living cells. The LiveFISH approach enables real-time live imaging of DNA and RNA during genome editing, transcription, and rearrangements in single cells.

scholarly journals Real-Time Monitoring and Detection of Single-Cell Level Cytokine Secretion Using LSPR Technology

Micromachines ◽  
2020 ◽  
Vol 11 (1) ◽  
pp. 107 ◽  
Author(s):  
Chen Zhu ◽  
Xi Luo ◽  
Wilfred Villariza Espulgar ◽  
Shohei Koyama ◽  
Atsushi Kumanogoh ◽  
...  
Keyword(s):  
Single Cell ◽  
Real Time ◽  
Single Cells ◽  
Clinical Diagnostics ◽  
Cytokine Secretion ◽  
Localized Surface Plasmon ◽  
Single Cell Level ◽  
Label Free ◽  
Real Time Monitoring ◽  
Cell Level

Cytokine secretion researches have been a main focus of studies among the scientists in the recent decades for its outstanding contribution to clinical diagnostics. Localized surface plasmon resonance (LSPR) technology is one of the conventional methods utilized to analyze these issues, as it could provide fast, label-free and real-time monitoring of biomolecule binding events. However, numerous LSPR-based biosensors in the past are usually utilized to monitor the average performance of cell groups rather than single cells. Meanwhile, the complicated sensor structures will lead to the fabrication and economic budget problems. Thus, in this paper, we report a simple synergistic integration of the cell trapping of microwell chip and gold-capped nanopillar-structured cyclo-olefin-polymer (COP) film for single cell level Interleukin 6 (IL-6) detection. Here, in-situ cytokine secreted from the trapped cell can be directly observed and analyzed through the peak red-shift in the transmittance spectrum. The fabricated device also shows the potential to conduct the real-time monitoring which would greatly help us identify the viability and biological variation of the tested single cell.

1618: Real-Time Monitoring of Nitric Oxide and Blood Flow During Ischemia-Reperfusion in the Rat Testis

2006 ◽  
Vol 175 (4S) ◽  
pp. 521-521
Author(s):  
Motoaki Saito ◽  
Tomoharu Kono ◽  
Yukako Kinoshita ◽  
Itaru Satoh ◽  
Keisuke Satoh
Keyword(s):  
Nitric Oxide ◽  
Blood Flow ◽  
Real Time ◽  
Ischemia Reperfusion ◽  
Real Time Monitoring ◽  
Rat Testis
Sign in / Sign up

Export Citation Format

Share Document