Gold-plated magnetic polymers for highly specific enrichment and label-free detection of blood biomarkers under physiological conditions

2014 ◽  
Vol 50 (70) ◽  
pp. 10066-10069 ◽  
Author(s):  
Seung-Ryong Kwon ◽  
Chang Su Jeon ◽  
Nam Young Hong ◽  
Kwang Pyo Kim ◽  
Inseong Hwang ◽  
...  
Keyword(s):  
Polymer Microspheres ◽  
Label Free ◽  
Nonspecific Binding ◽  
Blood Biomarkers ◽  
Physiological Conditions ◽  
Highly Efficient ◽  
Label Free Detection

Highly efficient prevention of nonspecific binding by gold-plated magnetic polymer microspheres enables bead-based label-free assays using MADLI MS under physiological conditions.

scholarly journals THz Detection of Biomolecules in Aqueous Environments—Status and Perspectives for Analysis Under Physiological Conditions and Clinical use

2021 ◽  
Author(s):  
Christian Weisenstein ◽  
Anna Katharina Wigger ◽  
Merle Richter ◽  
Robert Sczech ◽  
Anja Katrin Bosserhoff ◽  
...  
Keyword(s):  
Aqueous Media ◽  
Label Free ◽  
Physiological Conditions ◽  
Label Free Detection ◽  
Aqueous Environments ◽  
The Status ◽  
Detectable Concentration ◽  
Thz Sensing ◽  
Detection Of Biomolecules ◽  
Potential Use

AbstractBioanalytical THz sensing techniques have proven to be an interesting and viable tool for the label-free detection and analysis of biomolecules. However, a major challenge for THz bioanalytics is to perform investigations in the native aqueous environments of the analytes. This review recapitulates the status and future requirements for establishing THz biosensing as a complementary toolbox in the repertoire of standard bioanalytic methods. The potential use in medical research and clinical diagnosis is discussed. Under these considerations, this article presents a comprehensive categorization of biochemically relevant analytes that have been investigated by THz sensing techniques in aqueous media. The detectable concentration levels of ions, carbohydrates, (poly-)nucleotides, active agents, proteins and different biomacromolecules from THz experiments are compared to characteristic physiological concentrations and lower detection limits of state-of-the-art bioanalytical methods. Finally, recent experimental developments and achievements are discussed, which potentially pave the way for THz analysis of biomolecules under clinically relevant conditions.

Fabrication of Fe3O4@SiO2@Ag magnetic–plasmonic nanospindles as highly efficient SERS active substrates for label-free detection of pesticides

2017 ◽  
Vol 41 (4) ◽  
pp. 1582-1590 ◽  
Author(s):  
Qinye He ◽  
Aiwu Zhao ◽  
Lei Li ◽  
Henghui Sun ◽  
Dapeng Wang ◽  
...  
Keyword(s):  
Hot Spots ◽  
Label Free ◽  
Sers Substrates ◽  
Highly Efficient ◽  
Label Free Detection

Magnetic–plasmonic nanospindles serve as SERS substrates with controllable aggregation due to steady enrichment of mass molecules nearby abundant hot spots.

scholarly journals The Role of Surface Chemistry in the Efficacy of Protein and DNA Microarrays for Label-Free Detection: An Overview

Polymers ◽  
2021 ◽  
Vol 13 (7) ◽  
pp. 1026
Author(s):  
Elisa Chiodi ◽  
Allison M. Marn ◽  
Matthew T. Geib ◽  
M. Selim Ünlü
Keyword(s):  
Surface Chemistry ◽  
Surface Functionalization ◽  
Dna Microarrays ◽  
Label Free ◽  
Polymeric Coatings ◽  
Label Free Detection ◽  
Particle Imaging ◽  
Single Particle Imaging ◽  
The Impact

The importance of microarrays in diagnostics and medicine has drastically increased in the last few years. Nevertheless, the efficiency of a microarray-based assay intrinsically depends on the density and functionality of the biorecognition elements immobilized onto each sensor spot. Recently, researchers have put effort into developing new functionalization strategies and technologies which provide efficient immobilization and stability of any sort of molecule. Here, we present an overview of the most widely used methods of surface functionalization of microarray substrates, as well as the most recent advances in the field, and compare their performance in terms of optimal immobilization of the bioreceptor molecules. We focus on label-free microarrays and, in particular, we aim to describe the impact of surface chemistry on two types of microarray-based sensors: microarrays for single particle imaging and for label-free measurements of binding kinetics. Both protein and DNA microarrays are taken into consideration, and the effect of different polymeric coatings on the molecules’ functionalities is critically analyzed.

scholarly journals Label-Free Electrochemical Sensor for Rapid Bacterial Pathogen Detection Using Vancomycin-Modified Highly Branched Polymers

Sensors ◽  
2021 ◽  
Vol 21 (5) ◽  
pp. 1872
Author(s):  
Holger Schulze ◽  
Harry Wilson ◽  
Ines Cara ◽  
Steven Carter ◽  
Edward N. Dyson ◽  
...  
Keyword(s):  
Pathogen Detection ◽  
Electrochemical Impedance ◽  
Point Of Care ◽  
Branched Polymers ◽  
Label Free ◽  
Conformation Change ◽  
Gram Positive ◽  
Gram Positive Bacteria ◽  
Staphylococcus Carnosus ◽  
Label Free Detection

Rapid point of care tests for bacterial infection diagnosis are of great importance to reduce the misuse of antibiotics and burden of antimicrobial resistance. Here, we have successfully combined a new class of non-biological binder molecules with electrochemical impedance spectroscopy (EIS)-based sensor detection for direct, label-free detection of Gram-positive bacteria making use of the specific coil-to-globule conformation change of the vancomycin-modified highly branched polymers immobilized on the surface of gold screen-printed electrodes upon binding to Gram-positive bacteria. Staphylococcus carnosus was detected after just 20 min incubation of the sample solution with the polymer-functionalized electrodes. The polymer conformation change was quantified with two simple 1 min EIS tests before and after incubation with the sample. Tests revealed a concentration dependent signal change within an OD600 range of Staphylococcus carnosus from 0.002 to 0.1 and a clear discrimination between Gram-positive Staphylococcus carnosus and Gram-negative Escherichia coli bacteria. This exhibits a clear advancement in terms of simplified test complexity compared to existing bacteria detection tests. In addition, the polymer-functionalized electrodes showed good storage and operational stability.

H2O2-sensitive quantum dots for the label-free detection of glucose

Talanta ◽  
2010 ◽  
Vol 82 (3) ◽  
pp. 997-1002 ◽  
Author(s):  
Mei Hu ◽  
Jing Tian ◽  
Hao-Ting Lu ◽  
Li-Xing Weng ◽  
Lian-Hui Wang
Keyword(s):  
Quantum Dots ◽  
Label Free ◽  
Label Free Detection
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