Detection of cancer cells using triplex DNA molecular beacons based on expression of enhanced green fluorescent protein (eGFP)

2014 ◽  
Vol 50 (67) ◽  
pp. 9547-9549 ◽  
Author(s):  
Dongmei Xi ◽  
Xindong Wang ◽  
Shiyun Ai ◽  
Shusheng Zhang
Keyword(s):  
Green Fluorescent Protein ◽  
Cancer Cells ◽  
Enhanced Green Fluorescent Protein ◽  
Fluorescent Protein ◽  
Molecular Beacons ◽  
Triplex Dna ◽  
Cell Detection ◽  
Novel Strategy ◽  
Green Fluorescent ◽  
Cancer Cell Detection

A novel strategy was developed for cancer cell detection using triplex DNA based on expression of enhanced green fluorescent protein.

Establishment of successively transplantable rabbit VX2 cancer cells that express enhanced green fluorescent protein

2014 ◽  
Vol 48 (1) ◽  
pp. 13-23 ◽  
Author(s):  
Hisashi Oshiro ◽  
Hidenobu Fukumura ◽  
Kiyotaka Nagahama ◽  
Itaru Sato ◽  
Kei Sugiura ◽  
...  
Keyword(s):  
Green Fluorescent Protein ◽  
Cancer Cells ◽  
Enhanced Green Fluorescent Protein ◽  
Fluorescent Protein ◽  
Green Fluorescent

scholarly journals A Biosensor Platform for Metal Detection Based on Enhanced Green Fluorescent Protein

Sensors ◽  
2019 ◽  
Vol 19 (8) ◽  
pp. 1846 ◽  
Author(s):  
Woonwoo Lee ◽  
Hyojin Kim ◽  
Yerin Kang ◽  
Youngshim Lee ◽  
Youngdae Yoon
Keyword(s):  
Green Fluorescent Protein ◽  
Conformational Changes ◽  
Metal Binding ◽  
Enhanced Green Fluorescent Protein ◽  
Fluorescent Protein ◽  
Environmental Pollutants ◽  
Cadmium Detection ◽  
Novel Strategy ◽  
Split Egfp ◽  
Green Fluorescent

Microbial cell-based biosensors, which mostly rely on stress-responsive operons, have been widely developed to monitor environmental pollutants. Biosensors are usually more convenient and inexpensive than traditional instrumental analyses of environmental pollutants. However, the targets of biosensors are restricted by the limited number of genetic operon systems available. In this study, we demonstrated a novel strategy to overcome this limitation by engineering an enhanced green fluorescent protein (eGFP). It has been reported that combining two fragments of split-eGFP can form a native structure. Thus, we engineered new biosensors by inserting metal-binding loops (MBLs) between β-strands 9 and 10 of the eGFP, which then undergoes conformational changes upon interaction between the MBLs and targets, thereby emitting fluorescence. The two designed MLBs based on our previous study were employed as linkers between two fragments of eGFP. As a result, an Escherichia coli biosensor exhibited a fluorescent signal only when interacting with cadmium ions, revealing the prospect of a new biosensor for cadmium detection. Although this study is a starting stage for further developing biosensors, we believe that the proposed strategy can serve as basis to develop new biosensors to target various environmental pollutants.

scholarly journals Enhanced Green Fluorescent Protein (GFP) fluorescence after polyelectrolyte caging

2006 ◽  
Vol 14 (21) ◽  
pp. 9815 ◽  
Author(s):  
Alberto Diaspro ◽  
Silke Krol ◽  
Barbara Campanini ◽  
Fabio Cannone ◽  
Giuseppe Chirico
Keyword(s):  
Green Fluorescent Protein ◽  
Enhanced Green Fluorescent Protein ◽  
Fluorescent Protein ◽  
Gfp Fluorescence ◽  
Green Fluorescent

scholarly journals Development and Characterization of a cDNA-Launch Recombinant Simian Hemorrhagic Fever Virus Expressing Enhanced Green Fluorescent Protein: ORF 2b’ Is Not Required for In Vitro Virus Replication

Viruses ◽  
2021 ◽  
Vol 13 (4) ◽  
pp. 632
Author(s):  
Yingyun Cai ◽  
Shuiqing Yu ◽  
Ying Fang ◽  
Laura Bollinger ◽  
Yanhua Li ◽  
...  
Keyword(s):  
Green Fluorescent Protein ◽  
Cell Lines ◽  
Enhanced Green Fluorescent Protein ◽  
Fluorescent Protein ◽  
Infectious Clone ◽  
Hemorrhagic Fever ◽  
Simian Hemorrhagic Fever Virus ◽  
Hemorrhagic Fever Virus ◽  
Green Fluorescent

Simian hemorrhagic fever virus (SHFV) causes acute, lethal disease in macaques. We developed a single-plasmid cDNA-launch infectious clone of SHFV (rSHFV) and modified the clone to rescue an enhanced green fluorescent protein-expressing rSHFV-eGFP that can be used for rapid and quantitative detection of infection. SHFV has a narrow cell tropism in vitro, with only the grivet MA-104 cell line and a few other grivet cell lines being susceptible to virion entry and permissive to infection. Using rSHFV-eGFP, we demonstrate that one cricetid rodent cell line and three ape cell lines also fully support SHFV replication, whereas 55 human cell lines, 11 bat cell lines, and three rodent cells do not. Interestingly, some human and other mammalian cell lines apparently resistant to SHFV infection are permissive after transfection with the rSHFV-eGFP cDNA-launch plasmid. To further demonstrate the investigative potential of the infectious clone system, we introduced stop codons into eight viral open reading frames (ORFs). This approach suggested that at least one ORF, ORF 2b’, is dispensable for SHFV in vitro replication. Our proof-of-principle experiments indicated that rSHFV-eGFP is a useful tool for illuminating the understudied molecular biology of SHFV.

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