A label-free double-amplification system for sensitive detection of single-stranded DNA and thrombin by liquid chromatography-mass spectrometry

2014 ◽  
Vol 50 (69) ◽  
pp. 9846-9848 ◽  
Author(s):  
Wenbo Zhao ◽  
Zhen Qin ◽  
Chengsen Zhang ◽  
Meiping Zhao ◽  
Hai Luo
Keyword(s):  
Mass Spectrometry ◽  
Liquid Chromatography ◽  
Dna Probe ◽  
Sensitive Detection ◽  
Label Free ◽  
Liquid Chromatography Mass Spectrometry ◽  
Single Stranded Dna ◽  
Exonuclease Iii ◽  
Amplification System ◽  
Adenine Thymine

A label-free double amplification system has been developed by using a ternary DNA probe containing the poly(adenine-thymine) sequence assisted by exonuclease III degradation.

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