Protein electrochemistry using graphene-based nano-assembly: an ultrasensitive electrochemical detection of protein molecules via nanoparticle–electrode collisions

2014 ◽  
Vol 50 (60) ◽  
pp. 8197 ◽  
Author(s):  
Da Li ◽  
Jingquan Liu ◽  
Colin J. Barrow ◽  
Wenrong Yang
Keyword(s):  
Electrochemical Detection ◽  
Protein Electrochemistry ◽  
Protein Molecules

Aldehyde gold clusters for molecular labeling

1995 ◽  
Vol 53 ◽  
pp. 858-859
Author(s):  
James F. Hainfeld ◽  
Frederic R. Furuya
Keyword(s):  
Covalent Bond ◽  
Aldehyde Group ◽  
Gold Clusters ◽  
Side Reactions ◽  
Amino Groups ◽  
Sodium Cyanoborohydride ◽  
Schiff’S Base ◽  
Protein Molecules ◽  
Hydroxysuccinimide Esters ◽  
Primary Amino

Glutaraldehyde is a useful tissue and molecular fixing reagents. The aldehyde moiety reacts mainly with primary amino groups to form a Schiff's base, which is reversible but reasonably stable at pH 7; a stable covalent bond may be formed by reduction with, e.g., sodium cyanoborohydride (Fig. 1). The bifunctional glutaraldehyde, (CHO-(CH2)3-CHO), successfully stabilizes protein molecules due to generally plentiful amines on their surface; bovine serum albumin has 60; 59 lysines + 1 α-amino. With some enzymes, catalytic activity after fixing is preserved; with respect to antigens, glutaraldehyde treatment can compromise their recognition by antibodies in some cases. Complicating the chemistry somewhat are the reported side reactions, where glutaraldehyde reacts with other amino acid side chains, cysteine, histidine, and tyrosine. It has also been reported that glutaraldehyde can polymerize in aqueous solution. Newer crosslinkers have been found that are more specific for the amino group, such as the N-hydroxysuccinimide esters, and are commonly preferred for forming conjugates. However, most of these linkers hydrolyze in solution, so that the activity is lost over several hours, whereas the aldehyde group is stable in solution, and may have an advantage of overall efficiency.

ELECTROCHEMICAL DETECTION OF FLUOXETINE IN WATER USING A SILVER MODIFIED ZEOLITE-CARBON NANOFIBER ELECTRODE

2017 ◽  
Vol 16 (4) ◽  
pp. 829-836
Author(s):  
Florica Manea ◽  
Magdalena Ardelean ◽  
Aniela Pop ◽  
Rodica Pode ◽  
Joop Schoonman
Keyword(s):  
Electrochemical Detection ◽  
Carbon Nanofiber ◽  
Modified Zeolite
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