Hairpin DNA probes based on target-induced in situ generation of luminescent silver nanoclusters

2014 ◽  
Vol 50 (37) ◽  
pp. 4849 ◽  
Author(s):  
Yan Xiao ◽  
Zhengjun Wu ◽  
Kwok-Yin Wong ◽  
Zhihong Liu
Keyword(s):  
Dna Probes ◽  
Silver Nanoclusters ◽  
Hairpin Dna ◽  
In Situ Generation

New sensitive strategy for formaldehyde sensing by in situ generation of luminescent silver nanoclusters

2018 ◽  
Vol 296 (12) ◽  
pp. 1995-2004 ◽  
Author(s):  
Kanokwan Chaiendoo ◽  
Suthasinee Boonchiangma ◽  
Vinich Promarak ◽  
Wittaya Ngeontae
Keyword(s):  
Silver Nanoclusters ◽  
Formaldehyde Sensing ◽  
In Situ Generation

Characterizing Fluorescence Emissions of Hairpin DNA-Templated Silver Nanoclusters by cDNA Hybridization

2021 ◽  
Vol 21 (8) ◽  
pp. 4134-4138
Author(s):  
Seyeon Kim ◽  
Geumhee Ha ◽  
Jongback Gang
Keyword(s):  
Small Molecules ◽  
Fluorescence Intensity ◽  
Fluorescence Emission ◽  
Dna Probes ◽  
Silver Nanoclusters ◽  
Wild Type ◽  
Hairpin Dna ◽  
Stem Loop ◽  
Cdna Hybridization ◽  
Target Molecules

Stem-loop hairpin DNA probes have high hybridization specificity and unique selectivity to target molecules such as DNA and small molecules. DNA-templated silver nanoclusters (DNA-AgNCs) has been widely used to detect biomolecules of interest due to the photostable, bright, and efficient methods. In this study, we measured fluorescence emission of hairpin DNA upon hybridization with cDNA and mutant cDNA (cDNA-1) or mutant cDNA containing mismatched bases in the stem region (cDNA-2). Fluorescence intensity of hairpin DNA-AgNCs in the presence of cDNA was 1.80 times higher than that of hairpin DNA-AgNCs alone, but decreased to 66% in the presence of cDNA-1 containing mismatched base corresponding to the hairpin stem region. This study demonstrated that fluorescence intensities of hairpin DNA-AgNCs were dependent on hybridization with either wild-type and mutant cDNAs.

In situ generation of fluorescent silver nanoclusters in layer-by-layer assembled films

2013 ◽  
Vol 1 (10) ◽  
pp. 2036 ◽  
Author(s):  
Wenjing Zhang ◽  
Jia Song ◽  
Wang Liao ◽  
Ying Guan ◽  
Yongjun Zhang ◽  
...  
Keyword(s):  
Silver Nanoclusters ◽  
Layer By Layer ◽  
In Situ Generation

scholarly journals Fluorometric “Turn-On” glucose sensing through the in situ generation of silver nanoclusters

RSC Advances ◽  
2017 ◽  
Vol 7 (3) ◽  
pp. 1396-1400 ◽  
Author(s):  
Yang Chen ◽  
Yuanqing Sun ◽  
Rongjun Song ◽  
Shanliang Song ◽  
Yue Zhao ◽  
...  
Keyword(s):  
Fenton Reaction ◽  
Glucose Sensing ◽  
Silver Nanoclusters ◽  
Glucose Detection ◽  
Turn On ◽  
In Situ Generation ◽  
Ag Nanoclusters

A fluorometric “turn-on” glucose detection is performed based on the Fenton reaction which can trigger the generation of Ag nanoclusters.

Highly diastereo- and enantioselective organocatalytic synthesis of trifluoromethylated erythritols based on the in situ generation of unstable trifluoroacetaldehyde

2021 ◽  
Author(s):  
Kazumasa Funabiki ◽  
Toshiya Gotoh ◽  
Ryunosuke Kani ◽  
Toshiyasu Inuzuka ◽  
Yasuhiro Kubota
Keyword(s):  
Trifluoromethyl Group ◽  
In Situ Generation

A highly diastereo- and enantioselective organocatalytic method to synthesise erythritols bearing a trifluoromethyl group has been investigated.

Recent advances in Minisci-type reactions and applications in organic synthesis

2020 ◽  
Vol 24 ◽  
Author(s):  
Wengui Wang ◽  
Shoufeng Wang
Keyword(s):  
Free Radical ◽  
Hydrogen Atom ◽  
Organic Synthesis ◽  
Radical Reactivity ◽  
Atom Loss ◽  
Radical Generation ◽  
In Situ Generation ◽  
Thermal Cleavage ◽  
Synthetic Chemist

Abstract:: Minisci-type reactions have become widely known as reactions that involve the addition of carbon-centered radicals to basic heteroarenes followed by formal hydrogen atom loss. While the originally developed protocols for radical generation remain in active use today, in recent years by a new array of radical generation strategies allow use of a wider variety of radical precursors that often operate under milder and more benign conditions. New transformations based on free radical reactivity are now available to a synthetic chemist looking to utilize a Minisci-type reaction. Radical-generation methods based on photoredox catalysis and electrochemistry, which utilize thermal cleavage or the in situ generation of reactive radical precursors, have become popular approaches. Our review will cover the remarkably literature that has appeared on this topic in recent 5 years, from 2015-01 to 2020-01, in an attempt to provide guidance to the synthetic chemist, on both the challenges that have been overcome and applications in organic synthesis.

scholarly journals A residue-free approach to water disinfection using catalytic in situ generation of reactive oxygen species

2021 ◽  
Author(s):  
Thomas Richards ◽  
Jonathan H. Harrhy ◽  
Richard J. Lewis ◽  
Alexander G. R. Howe ◽  
Grzegorz M. Suldecki ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Reactive Oxygen ◽  
Water Disinfection ◽  
Oxygen Species ◽  
In Situ Generation

In Situ Generation of Unstable Difluoromethylphosphonate-Containing Diazoalkanes and Their Use in [3 + 2] Cycloaddition Reactions with Vinyl Sulfones

2021 ◽  
Vol 23 (3) ◽  
pp. 1130-1134
Author(s):  
Haibo Mei ◽  
Li Wang ◽  
Romana Pajkert ◽  
Qian Wang ◽  
Jingcheng Xu ◽  
...  
Keyword(s):  
Cycloaddition Reactions ◽  
In Situ Generation

Experimental Investigation of Laser Sintering of Conductive Adhesive for Functional Prototypes Produced by Embedding Stereolithography

2014 ◽  
Vol 1038 ◽  
pp. 75-81
Author(s):  
Bernd Niese ◽  
Philipp Amend ◽  
Uwe Urmoneit ◽  
Stephan Roth ◽  
Michael Schmidt
Keyword(s):  
Thermal Damage ◽  
Laser Sintering ◽  
Conductive Adhesive ◽  
Flexible Production ◽  
Sintering Process ◽  
Building Process ◽  
In Situ Generation ◽  
Functional Components

Embedding stereolithography (eSLA) is an additive, hybrid process, which provides a flexible production of 3D components and the ability to integrate electrical and optical conductive structures and functional components within parts. However, the embedding of conductive circuits in stereolithography (SLA) parts assumes usage of process technologies, which enables their direct integration of conductive circuits during the layer-wise building process. In this context, a promising method for in-situ generation of conductive circuits is dispensing of conductive adhesive on the current surface of the SLA part and its subsequent sintering. In this paper, the laser sintering (λ = 355 nm) of conductive adhesive mainly consisting of silver nanoparticles is investigated. The work intends to evaluate the curing behavior of the conductive adhesive, the beam-matter-interactions and the thermal damage of the SLA substrate. The investigations revealed a fast and flexible laser sintering process for the generation of conductive circuits with sufficient electrical conductivity and sufficient current capacity load. In this context, a characterization of the conductive structures is done by measuring their electrical resistance and their potential current capacity load.

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