Identification of isoafricanol and its terpene cyclase in Streptomyces violaceusniger using CLSA-NMR

2014 ◽  
Vol 50 (32) ◽  
pp. 4228 ◽  
Author(s):  
Ramona Riclea ◽  
Christian A. Citron ◽  
Jan Rinkel ◽  
Jeroen S. Dickschat
Keyword(s):  
Terpene Cyclase ◽  
Streptomyces Violaceusniger

ChemInform Abstract: Identification of Isoafricanol (I) and Its Terpene Cyclase in Streptomyces violaceusniger Using CLSA-NMR.

ChemInform ◽  
2014 ◽  
Vol 45 (37) ◽  
pp. no-no
Author(s):  
Ramona Riclea ◽  
Christian A. Citron ◽  
Jan Rinkel ◽  
Jeroen S. Dickschat
Keyword(s):  
Terpene Cyclase ◽  
Streptomyces Violaceusniger

A putative terpene cyclase, vir4, is responsible for the biosynthesis of volatile terpene compounds in the biocontrol fungus Trichoderma virens

2013 ◽  
Vol 56 ◽  
pp. 67-77 ◽  
Author(s):  
Frankie K. Crutcher ◽  
Alexandra Parich ◽  
Rainer Schuhmacher ◽  
Prasun K. Mukherjee ◽  
Susanne Zeilinger ◽  
...  
Keyword(s):  
Trichoderma Virens ◽  
Terpene Cyclase ◽  
Volatile Terpene

Substrate Pre-Folding and Water Molecule Organization Matters for Terpene Cyclase Catalyzed Conversion of Unnatural Substrates

2016 ◽  
Vol 1 (13) ◽  
pp. 3589-3593 ◽  
Author(s):  
Stephan C. Hammer ◽  
Per-Olof Syrén ◽  
Bernhard Hauer
Keyword(s):  
Water Molecule ◽  
Terpene Cyclase

scholarly journals Silent catalytic promiscuity in the high-fidelity terpene cyclase δ-cadinene synthase

2019 ◽  
Vol 17 (5) ◽  
pp. 1206-1214 ◽  
Author(s):  
Marianna Loizzi ◽  
David J. Miller ◽  
Rudolf K. Allemann
Keyword(s):  
High Fidelity ◽  
Catalytic Promiscuity ◽  
Terpene Cyclase

Aza-analogues of carbocations inhibit δ-cadinene synthase: 1,6-cyclisation.

Diversity of members of the Streptomyces violaceusniger 16S rRNA gene clade in the legumes rhizosphere in Turkey

2010 ◽  
Vol 60 (4) ◽  
pp. 669-684 ◽  
Author(s):  
Nevzat Sahin ◽  
Anil Sazak ◽  
Kiymet Güven ◽  
Meral Dogramaci
Keyword(s):  
16S Rrna ◽  
16S Rrna Gene ◽  
Rrna Gene ◽  
Streptomyces Violaceusniger

scholarly journals Protonation-Initiated Cyclization by a Class II Terpene Cyclase Assisted by Tunneling

ChemBioChem ◽  
2017 ◽  
Vol 18 (23) ◽  
pp. 2301-2305 ◽  
Author(s):  
Adam Eriksson ◽  
Charlotte Kürten ◽  
Per-Olof Syrén
Keyword(s):  
Class Ii ◽  
Terpene Cyclase

Biocontrol of wood-rotting fungi withStreptomyces violaceusnigerXL-2

2006 ◽  
Vol 52 (9) ◽  
pp. 805-808 ◽  
Author(s):  
Nilanshu Shekhar ◽  
Debaditya Bhattacharya ◽  
Dishant Kumar ◽  
Rajinder K Gupta
Keyword(s):  
Biocontrol Agent ◽  
Sodium Dodecyl ◽  
Deae Cellulose ◽  
Industrial Applications ◽  
Stress Factors ◽  
Antihypertensive Activity ◽  
Growth Media ◽  
Fungal Cell ◽  
Wide Range ◽  
Streptomyces Violaceusniger

During the previous decade, chitinases have received increased attention because of their wide range of applications. Chito-oligomers produced by enzymatic hydrolysis of chitin have been of interest in recent years because of their broad applications in medical, agricultural, and industrial applications, such as antibacterial, antifungal, hypo cholesterolemic, and antihypertensive activity, and as food quality enhancer. Fungal cell walls being rich in chitin also enable the use of chitinases in biocontrol of fungal pathogens, as bio-fungicides. An actinomycete was isolated from the bark of trees of Dehradun in India and was later identified as Streptomyces violaceusniger. This strain exhibits strong antagonism towards various wood-rotting fungi, such as Phanerochaete chrysosporium, Postia placenta, Coriolus versicolor, and Gloeophyllum trabeum. Further, studies showed an extracellular bioactive compound was responsible for the antagonism. The conditions for the production of this biocontrol agent were optimized, and the effects of various stress factors (like nitrogen-deficient media, carbon-deficient media, etc.) were studied. The presence of chitin in the growth media was found to be an essential factor for the active production of the biocontrol agent. The pH and temperature optima for the biocontrol agent were determined. Purification and characterization of this specific biocontrol agent was performed through anion exchange chromatography using a DEAE–cellulose column, and a single protein band was obtained on a 10% sodium dodecyl sulfate – polyacrylamide gel. The protein was later identified as a 28 kDa endo chitinase by MALDI–TOF (matrix-assisted laser desorption ionization – time of flight) and by a chitobiose activity assay.Key words: actinomycetes, biocontrol agents, Streptomyces violaceusniger, chitinase.

scholarly journals BE-24566B, a New Antibiotic Produced by Streptomyces violaceusniger.

1995 ◽  
Vol 48 (12) ◽  
pp. 1506-1508 ◽  
Author(s):  
KATSUHISA KOJIRI ◽  
SHIGERU NAKAJIMA ◽  
AISAKU FUSE ◽  
HAJIME SUZUKI ◽  
HIROYUKI SUDA
Keyword(s):  
Streptomyces Violaceusniger

scholarly journals Synthesis of Trichodermin Derivatives and Their Antimicrobial and Cytotoxic Activities

Molecules ◽  
2019 ◽  
Vol 24 (20) ◽  
pp. 3811
Author(s):  
Javier E. Barúa ◽  
Mercedes de la Cruz ◽  
Nuria de Pedro ◽  
Bastien Cautain ◽  
Rosa Hermosa ◽  
...  
Keyword(s):  
Human Hepatocytes ◽  
Bioactive Molecules ◽  
Cytotoxic Activities ◽  
Terpene Cyclase ◽  
Trichodiene Synthase ◽  
Specific Step ◽  
Ic50 Values ◽  
Selective Antimicrobial Activity ◽  
Tumorigenic Cell Line ◽  
Mcf 7

Trichothecene mycotoxins are recognized as highly bioactive compounds that can be used in the design of new useful bioactive molecules. In Trichoderma brevicompactum, the first specific step in trichothecene biosynthesis is carried out by a terpene cyclase, trichodiene synthase, that catalyzes the conversion of farnesyl diphosphate to trichodiene and is encoded by the tri5 gene. Overexpression of tri5 resulted in increased levels of trichodermin, a trichothecene-type toxin, which is a valuable tool in preparing new molecules with a trichothecene skeleton. In this work, we developed the hemisynthesis of trichodermin and trichodermol derivatives in order to evaluate their antimicrobial and cytotoxic activities and to study the chemo-modulation of their bioactivity. Some derivatives with a short chain at the C-4 position displayed selective antimicrobial activity against Candida albicans and they showed MIC values similar to those displayed by trichodermin. It is important to highlight the cytotoxic selectivity observed for compounds 9, 13, and 15, which presented average IC50 values of 2 μg/mL and were cytotoxic against tumorigenic cell line MCF-7 (breast carcinoma) and not against Fa2N4 (non-tumoral immortalized human hepatocytes).

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