A novel micro pressurized liquid extraction method for very rapid solid sample preparation

2015 ◽  
Vol 7 (4) ◽  
pp. 1509-1516 ◽  
Author(s):  
Fadi L. Alkhateeb ◽  
Kevin B. Thurbide
Keyword(s):  
Sample Preparation ◽  
Extraction Method ◽  
Rapid Heating ◽  
Liquid Extraction ◽  
Solid Sample ◽  
Pressurized Liquid Extraction ◽  
Static Mode

A novel micro pressurized liquid extraction (μPLE) method is introduced, which employs rapid heating in a static mode to remove analytes from 5–10 mg samples in as little as 10 seconds using only 125 μL of solvent.

Chlorogenic Acid Stability in Pressurized Liquid Extraction Conditions

2015 ◽  
Vol 98 (2) ◽  
pp. 415-421 ◽  
Author(s):  
Dorota Wianowska ◽  
Rafał Typek ◽  
Andrzej L Dawidowicz
Keyword(s):  
Sample Preparation ◽  
Higher Plants ◽  
Food Additives ◽  
Transformation Process ◽  
Liquid Extraction ◽  
Chemical Degradation ◽  
Pressurized Liquid Extraction ◽  
Plant Sample ◽  
Natural Occurrence ◽  
Reaction With Water

Abstract Chlorogenic acids (CQAs) are phenolic compounds naturally occurring in all higher plants. They are potentially useful in pharmaceuticals, foodstuffs, food additives, and cosmetics due to their recently suggested biomedical activity. Hence, research interestin CQA properties, their isomers, and natural occurrence has been growing. Pressurized liquid extraction(PLE) is regarded as an effective and quick sample preparation method in plant analysis. The short time of PLE decreases the risk of chemical degradation ofextracted compounds, thus increasing the attractiveness of its application. However, PLE applied for plant sample preparation is not free from limitations. We found that trans-5-O-caffeoylquinic acid (trans-5-CQA), the main CQA isomer, isomerizes to 3- and 4-O-caffeoylquinic acids and undergoes transesterification, hydrolysis, and reaction with water even in rapid PLE. Moreover, the number and concentration of trans-5-CQA derivatives formed in PLE strongly depends on extractant composition, its pH, and extraction time and temperature. It was not possible to find the PLE conditions in which the transformation process of trans-5-CQA would be eliminated.

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