Determination of polyphenols in the pear pulp matrix by solvent extraction and liquid chromatography with UV-Vis detection

2014 ◽  
Vol 6 (24) ◽  
pp. 9769-9776 ◽  
Author(s):  
Maria Raja ◽  
Joel Hernández-Revelles ◽  
Santiago Hernández-Cassou ◽  
Javier Saurina
Keyword(s):  
Liquid Chromatography ◽  
Solvent Extraction ◽  
Analytical Method ◽  
Polyphenolic Compounds

A new analytical method for the determination of polyphenolic compounds in pear pulp was developed here.

scholarly journals Gas Chromatographic Determination of Traces of Accelerants in Physical Evidence

1972 ◽  
Vol 55 (4) ◽  
pp. 840-845 ◽  
Author(s):  
Charles R Midkiff ◽  
Willard D Washington
Keyword(s):  
Liquid Chromatography ◽  
Solvent Extraction ◽  
Vapor Phase ◽  
Selection Criteria ◽  
Chromatographic Determination ◽  
Operating Parameters ◽  
Gas Liquid Chromatography ◽  
Internal Standards ◽  
Physical Evidence

Techniques developed for use with gas-liquid chromatography for the examination of evidence collected at the scene of suspected arson fires and firebombings are discussed. Both solvent extraction and vapor phase examinations are employed. Internal standards are used for the identification of specific components in actual samples to allow confirmation of hydrocarbon type, e.g., gasoline and kerosene. Operating parameters and solvent selection criteria are included. Results obtained from known materials and residual hydrocarbons in actual samples are compared.

scholarly journals RAPID ANALYTICAL METHOD FOR ASSAY DETERMINATION FOR PROCHLORPERAZINE EDISYLATE DRUG SUBSTANCES BY ULTRA PERFORMANCE LIQUID CHROMATOGRAPHY

2017 ◽  
Vol 9 (4) ◽  
pp. 118 ◽  
Author(s):  
Kishorkumar L. Mule
Keyword(s):  
Liquid Chromatography ◽  
Trifluoroacetic Acid ◽  
Analytical Method ◽  
Mobile Phase ◽  
Ultra Performance Liquid Chromatography ◽  
Drug Substance ◽  
Assay Method ◽  
Column Temperature ◽  
Drug Substances

Objective: To develop and validate new, simple and rapid assay method for Prochlorperazine edisylate drug substance by UPLC as per ICH guidelines.Methods: Ultra performance liquid chromatographic method was developed, optimized and validated on Acquity UPLC by using Acquity BDH300 C4 (100 x 2.1 mm) 1.7µ column. 3.85g ammonium acetate in 1000 ml of water add 0.5 ml trifluoroacetic acid and 1 ml triethylamine (Mobile phase A): 0.5 ml trifluoroacetic acid in 1000 ml acetonitrile mobile phase (Mobile phase B) with gradient program. Detector wavelength 254 nm and column temperature 30 °C.Results: Linearity study was carried out for prochlorperazine edisylate, linearity was calculated from 80 % level to 120% with respect to specification level. The correlation coefficient (r) = 0.999 was proved that the method is robust. The resolution between known impurities and Prochlorperazine edisylate found more than 2.5, it was evident from specificity test that Prochlorperazine edisylate peak are well separated from its related impurities, hence the method is specific. Prochlorperazine edisylate sample solution and mobile phase were found to be stable for at least 3 d.Conclusion: A new, simple and rapid method has been developed and validated for assay determination of prochlorperazine edisylate in drug substance by Ultra Performance Liquid Chromatography (UPLC). The analytical method was developed and validated as per ICH guidelines. The developed method can be used for the fast assay determination of prochlorperazine edisylate drug substances in research laboratories and in the pharmaceutical industry. 

scholarly journals Method for Determination of Xanthene Dyes in Guava Fruits and Its Application in a Field Dissipation Study

2000 ◽  
Vol 83 (3) ◽  
pp. 563-568
Author(s):  
Jocelyn P Alcantara-Licudine ◽  
Ngoc Lan Bui ◽  
Qing X Li ◽  
Grant T McQuate ◽  
Steven L Peck
Keyword(s):  
Liquid Chromatography ◽  
Solvent Extraction ◽  
Fruit Fly ◽  
Average Concentration ◽  
Xanthene Dyes ◽  
Guava Fruit ◽  
Zone Electrophoresis ◽  
Capillary Zone ◽  
Tephritid Fruit Flies

Abstract Xanthene dyes, i.e., phloxine B and uranine or phloxine B alone, are phototoxic to tephritid fruit flies infesting guava fruits. An analytical method was developed for determination of residues of these dyes used in bait solutions for suppression of the tephritid fruit fly population in guava fruits. The procedure involved solvent extraction, anionexchange cleanup, and determination by liquid chromatography or capillary zone electrophoresis. The dyes were extracted from 50 g guava fruit at 45°C with 400 mL methanol–acetonitrile (1 + 1) and 5 g magnesium oxide added as an alkaline and clarifying agent. The guava extract was adjusted to pH 8.5 and subjected to an amino column cleanup. Average recoveries of xanthene dyes added to guava purees ranged from 77 to 99% for phloxine B and from 79 to 102% for uranine at spiking levels of 0.05–1.00 μg/g. The method was applied to the determination of phloxine B residues in guava fruits collected from a dye-sprayed orchard. After phloxine B was applied at a rate of 62.5 g/ha for 14 weekly sprayings, it was found on guava fruits at an average concentration of 111 ± 18 ng/g 4 h after the 11th spraying. The concentration of phloxine B was 426 ± 94 ng/g in selected fruits with high deposits of the dye 4 h after spraying. Average concentrations of phloxine B 5 days after the 7th and 14th sprayings were 29 ± 7 and 19 ± 8 ng/g, respectively.

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