A sensitive heterogeneous biotin–streptavidin enzyme-linked immunosorbent assay for the determination of di-(2-ethylhexyl)phthalate (DEHP) in beverages using a specific polyclonal antibody

2014 ◽  
Vol 6 (24) ◽  
pp. 9807-9815 ◽  
Author(s):  
Ruiyan Sun ◽  
Huisheng Zhuang
Keyword(s):  
Molecular Weight ◽  
High Molecular Weight ◽  
Immunosorbent Assay ◽  
Polyclonal Antibody ◽  
Phthalic Acid ◽  
Enzyme Linked Immunosorbent Assay ◽  
Ethylhexyl Phthalate ◽  
Specific Polyclonal Antibody ◽  
Long Chain

Di-(2-ethylhexyl)phthalate (DEHP) is one of the long-chain or high-molecular-weight compounds of the phthalic acid diester (PAEs) family, which is the most commonly used plasticizer and additive.

An indirect competitive enzyme-linked immunosorbent assay for the determination of 3,4-dichlorobiphenyl in sediment using a specific polyclonal antibody

2014 ◽  
Vol 6 (3) ◽  
pp. 893-899 ◽  
Author(s):  
Guangxin Yang ◽  
Huisheng Zhuang ◽  
Hanyu Chen ◽  
Xianyin Ping
Keyword(s):  
Immunosorbent Assay ◽  
Polyclonal Antibody ◽  
Enzyme Linked Immunosorbent Assay ◽  
Sediment Samples ◽  
Antibody Targeting ◽  
Specific Polyclonal Antibody ◽  
Dioxin Like Pcb

A specific polyclonal antibody targeting non-dioxin-like PCB 3,4-dichlorobiphenyl (PCB12) was obtained, and a sensitive ic-ELISA was developed for the determination of PCB12 in sediment samples.

scholarly journals Novel Polyclonal Antibody Raised against Tetrodotoxin Using Its Haptenic Antigen Prepared from 4,9-anhydrotetrodotoxin Reacted with 1,2-Ethaneditiol and Further Reacted with Keyhole Limpet Hemocyanin

Toxins ◽  
2019 ◽  
Vol 11 (10) ◽  
pp. 551 ◽  
Author(s):  
Sato ◽  
Takaishi ◽  
Yasumoto ◽  
Watabe
Keyword(s):  
Immunosorbent Assay ◽  
Polyclonal Antibody ◽  
Specific Antibody ◽  
Metabolic Pathways ◽  
Keyhole Limpet Hemocyanin ◽  
Enzyme Linked Immunosorbent Assay ◽  
Elisa System ◽  
One Step ◽  
Analytical Procedures ◽  
Specific Polyclonal Antibody

A novel polyclonal antibody against tetrodotoxin (TTX) was raised using its haptenic antigen, where 4,9-anhydroTTX was reacted with 1,2-ethanedithiol and this derivative was further reacted with keyhole limpet hemocyanin (KLH). This newly designed antigen (KLH-TTX) was inoculated into rabbits, resulting in the production of the specific polyclonal antibody, which reacted well with TTX and its analogs, 4-epiTTX, 11-oxoTTX and 5,6,11-trideoxyTTX, except for 4,9-anhydroTTX. The enzyme-linked immunosorbent assay (ELISA) system using this specific antibody was also developed in the present study. This newly developed polyclonal antibody with analytical procedures using direct one-step ELISA is useful to detect TTX and its analogs in toxic organisms and also disclose the mechanisms involved in their metabolic pathways and accumulation of TTX.

Highly selective and sensitive determination of deoxymiroestrol using a polyclonal antibody-based enzyme-linked immunosorbent assay

Talanta ◽  
2013 ◽  
Vol 114 ◽  
pp. 73-78 ◽  
Author(s):  
Gorawit Yusakul ◽  
Orapin Udomsin ◽  
Thaweesak Juengwatanatrakul ◽  
Hiroyuki Tanaka ◽  
Chaiyo Chaichantipyuth ◽  
...  
Keyword(s):  
Immunosorbent Assay ◽  
Polyclonal Antibody ◽  
Enzyme Linked Immunosorbent Assay ◽  
Sensitive Determination

scholarly journals Enzyme-linked immunosorbent assay for the determination of isoflavones in alimentary important plants

2004 ◽  
Vol 22 (SI - Chem. Reactions in Foods V) ◽  
pp. S199-S202 ◽  
Author(s):  
M. Vítková ◽  
Z. Macková ◽  
R. Koblovská ◽  
O. Lapčík
Keyword(s):  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Medicago Sativa ◽  
Immunosorbent Assay ◽  
Polyclonal Antibody ◽  
Bovine Serum ◽  
Enzyme Linked Immunosorbent Assay ◽  
Acceptable Range ◽  
Carboxy Methyl

The development of polyclonal antibody-based enzyme-linked immunosorbent assays (ELISA) for the determination of individual isoflavones, i.e. daidzein, genistein and biochanin and their homologues, is presented in this work. Isoflavone conjugates with bovine serum albumin were used as immunogens, coupled at the position C 7 and C 4’ via a carboxy methyl spacer. The developed ELISAs are highly specific, I<sub>50</sub> values of the standard curves range between 0.3–1.2 ng/ml. The cross reactivities to other isoflavones are in acceptable range and the interference of non-isoflavonoid molecules is negligible. The immunoassays have been used for monitoring of changes in isoflavone levels in alimentary important plants, such as Medicago sativa, during germination; and during different vegetation stages of the Rutaceae family plants.

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