Screening and structural characterization of potential α-glucosidase inhibitors from Radix Astragali flavonoids extract by ultrafiltration LC-DAD-ESI-MSn

2015 ◽  
Vol 7 (1) ◽  
pp. 123-128 ◽  
Author(s):  
Wenyue Jiang ◽  
Hong Kan ◽  
Pengdong Li ◽  
Shu Liu ◽  
Zhongying Liu
Keyword(s):  
Diabetes Mellitus ◽  
Structural Characterization ◽  
Radix Astragali ◽  
Glucosidase Activity ◽  
Glucosidase Inhibitors

Inhibition of intestinal α-glucosidase activity is one important mechanism for the management of diabetes mellitus (DM).

scholarly journals Different Proportions of Huangqi (Radix Astragali Mongolici) and Honghua (Flos Carthami) Injection onα-Glucosidase andα-Amylase Activities

2015 ◽  
Vol 2015 ◽  
pp. 1-7 ◽  
Author(s):  
Hui Liao ◽  
Linda Banbury
Keyword(s):  
Amylase Activity ◽  
Positive Control ◽  
Significant Inhibition ◽  
Radix Astragali ◽  
Glucosidase Activity ◽  
Glucosidase Inhibitors ◽  
Flos Carthami ◽  
Simultaneous Inhibition ◽  
Amylase Inhibitors ◽  
Hydrolysis Of

Objective.To study the effect of different proportions of Huangqi (Radix Astragali Mongolici) and Honghua (Flos Carthami) injection onα-glucosidase andα-amylase activity simultaneously.Methods.The injections were prepared according to the standards of the China Food and Drug Administration. The assay for potentialα-glucosidase inhibitors was based on the hydrolysis of 4-methylumbelliferyl-α-D-glucopyranoside (4-MUG). Theα-amylase EnzChek assay kit was used to determine potentialα-amylase inhibitors. Acarbose was the positive control.Results.The half maximal (50%) inhibitory concentration (IC50) of acarbose againstα-glucosidase andα-amylase was (1.8±0.4)μg/mL and (227±32)μg/mL, respectively. Honghua showed significant inhibition ofα-glucosidase activity compared with Huangqi (P<0.01). Honghua inhibitedα-amylase activity, but Huangqi did not. IC50s forα-glucosidase inhibition by mixtures at 10 : 1, 5 : 1, and 2 : 1 were significantly lower than those at the 20 : 1 mixture (P<0.01).α-Amylase inhibition by the 2 : 1 mixture was significantly higher than that by the 20 : 1, 10 : 1, and 5 : 1 mixtures at 500 μg/mL and 1000 μg/mL (P<0.01), with 5 : 1 significantly higher than 20 : 1 and 10 : 1 at 1000 μg/mL (P<0.01).Conclusion.Honghua significantly inhibitedα-glucosidase activity compared with Huangqi (P<0.01). For simultaneous inhibition ofα-glucosidase andα-amylase activities, the mixtures at 2 : 1 and 5 : 1 exhibited significant effects compared with those at 20 : 1 (P<0.01).

New ultrastructural findings about Borrelia burgdorferi by cryofixation, negative staining, thin sectioning and scanning techniques

1990 ◽  
Vol 48 (3) ◽  
pp. 582-583
Author(s):  
S. F. Hayes ◽  
M. D. Corwin ◽  
T. G. Schwan ◽  
D. W. Dorward ◽  
W. Burgdorfer
Keyword(s):  
Lyme Disease ◽  
Borrelia Burgdorferi ◽  
Structural Characterization ◽  
Negative Staining ◽  
Low Speed ◽  
Thin Sectioning ◽  
Ultrastructural Findings

Characterization of Borrelia burgdorferi strains by means of negative staining EM has become an integral part of many studies related to the biology of the Lyme disease organism. However, relying solely upon negative staining to compare new isolates with prototype B31 or other borreliae is often unsatisfactory. To obtain more satisfactory results, we have relied upon a correlative approach encompassing a variety EM techniques, i.e., scanning for topographical features and cryotomy, negative staining and thin sectioning to provide a more complete structural characterization of B. burgdorferi.For characterization, isolates of B. burgdorferi were cultured in BSK II media from which they were removed by low speed centrifugation. The sedimented borrelia were carefully resuspended in stabilizing buffer so as to preserve their features for scanning and negative staining. Alternatively, others were prepared for conventional thin sectioning and for cryotomy using modified procedures. For thin sectioning, the fixative described by Ito, et al.

Structural characterization of the PPIase domain of FKBP51, a cochaperone of human Hsp90

2011 ◽  
Vol 44 (06) ◽  
Author(s):  
A Bracher ◽  
C Kozany ◽  
AK Thost ◽  
F Hausch
Keyword(s):  
Structural Characterization ◽  
Ppiase Domain

Structural characterization of dendrobine-type sesquiterpene alkaloids from the stems of Dendrobium nobile using LC-MS/MS

Planta Medica ◽  
2014 ◽  
Vol 80 (10) ◽  
Author(s):  
YH Wang ◽  
B Avula ◽  
N Abe ◽  
F Wei ◽  
M Wang ◽  
...  
Keyword(s):  
Structural Characterization ◽  
Dendrobium Nobile

Structural characterization of monacolin compounds from red yeast rice by liquid chromatography and tandem mass

Planta Medica ◽  
2015 ◽  
Vol 81 (11) ◽  
Author(s):  
YH Wang ◽  
B Avula ◽  
Z Zhang ◽  
M Wang ◽  
S Sagi ◽  
...  
Keyword(s):  
Liquid Chromatography ◽  
Structural Characterization ◽  
Red Yeast Rice ◽  
Tandem Mass ◽  
Red Yeast
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