3D darkfield imaging and single particle tracking of peptide-coated nanocargoes in live cells

2014 ◽  
Vol 6 (23) ◽  
pp. 9202-9205 ◽  
Author(s):  
Hui Shen ◽  
Bin Xiong ◽  
Ruili Xu ◽  
Xiaodong Cheng ◽  
Yan He ◽  
...  
Keyword(s):  
Particle Tracking ◽  
Single Particle ◽  
Single Particle Tracking ◽  
Live Cells ◽  
Darkfield Imaging

scholarly journals Compact and Blinking-Suppressed Quantum Dots for Single-Particle Tracking in Live Cells

2014 ◽  
Vol 118 (49) ◽  
pp. 14140-14147 ◽  
Author(s):  
Lucas A. Lane ◽  
Andrew M. Smith ◽  
Tianquan Lian ◽  
Shuming Nie
Keyword(s):  
Quantum Dots ◽  
Particle Tracking ◽  
Single Particle ◽  
Single Particle Tracking ◽  
Live Cells

scholarly journals Single-particle tracking of murine polyoma virus-like particles on live cells and artificial membranes

2005 ◽  
Vol 102 (42) ◽  
pp. 15110-15115 ◽  
Author(s):  
H. Ewers ◽  
A. E. Smith ◽  
I. F. Sbalzarini ◽  
H. Lilie ◽  
P. Koumoutsakos ◽  
...  
Keyword(s):  
Particle Tracking ◽  
Single Particle ◽  
Single Particle Tracking ◽  
Polyoma Virus ◽  
Live Cells ◽  
Artificial Membranes ◽  
Virus Like Particles

scholarly journals Live-cell single particle tracking of PRC1 reveals a highly dynamic system with low target site occupancy

2020 ◽  
Author(s):  
Miles K. Huseyin ◽  
Robert J. Klose
Keyword(s):  
Particle Tracking ◽  
Single Particle ◽  
Genome Engineering ◽  
Target Genes ◽  
Embryonic Stem ◽  
Site Occupancy ◽  
Single Particle Tracking ◽  
Gene Repression ◽  
Live Cells ◽  
Chromatin Binding

AbstractPolycomb repressive complex 1 (PRC1) is an essential chromatin-based repressor of gene transcription. However, how PRC1 engages with chromatin to identify its target genes and achieve gene repression remains poorly defined, representing a major hurdle to our understanding of Polycomb system function. Here we use genome engineering and single particle tracking to dissect how PRC1 binds to chromatin in live mouse embryonic stem cells. We reveal that PRC1 is highly dynamic, with only a small fraction stably interacting with chromatin. By integrating subunit-specific dynamics, chromatin binding, and abundance measurements, we discover that PRC1 exhibits surprisingly low occupancy at target sites. Furthermore, we employ perturbation approaches to uncover how specific components of PRC1 define its kinetics and chromatin binding. Together, these discoveries provide a quantitative understanding of chromatin binding by PRC1 in live cells, and suggests that chromatin modification, as opposed to PRC1 complex occupancy, is central to gene repression.

scholarly journals A new Paradigm in Single-Particle Tracking in Live Cells: Onset of Ergodicity Breaking

2011 ◽  
Vol 100 (3) ◽  
pp. 251a
Author(s):  
Diego Krapf ◽  
Aubrey V. Weigel ◽  
Blair Simon ◽  
Michael M. Tamkun
Keyword(s):  
Particle Tracking ◽  
Single Particle ◽  
Single Particle Tracking ◽  
Live Cells ◽  
New Paradigm ◽  
Ergodicity Breaking
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