Bottom-up mass spectrometric sequencing of microRNA

2014 ◽  
Vol 6 (21) ◽  
pp. 8829-8839 ◽  
Author(s):  
Dickson M. Wambua ◽  
Masaaki Ubukata ◽  
John Dane ◽  
Robert B. Cody ◽  
Norman H. L. Chiu
Keyword(s):  
High Temperature ◽  
Mass Spectrometric ◽  
Nucleotide Identity ◽  
Sequence Coverage ◽  
Bottom Up ◽  
Monoisotopic Mass ◽  
Maldi Matrix

By using the acidity of MALDI matrix at high temperature, sequencing ladders of miRNA were generated. The accurate monoisotopic mass of each RNA fragment was measured by MALDI SpiralTOF. Analysis of nucleotide identity and complementary sequencing ladders allowed 100% sequence coverage and accuracy to be achieved.

scholarly journals Solution-Processable Porous Graphitic Carbon from Bottom-Up Synthesis and Low-Temperature Graphitization

2021 ◽  
Author(s):  
Sai Che ◽  
Lei Fang ◽  
Sarbajit Banerjee ◽  
Mohammed Al-Hashimi ◽  
Zi-Hao Guo ◽  
...  
Keyword(s):  
High Temperature ◽  
Low Temperature ◽  
Porous Graphitic Carbon ◽  
Graphitic Carbon ◽  
Bottom Up ◽  
Solution Processable

It is urgently desired yet challenging to synthesize porous graphitic carbon (PGC) in a bottom-up manner while circumventing the need for high-temperature pyrolysis. Here we present an effective and scalable...

High‐temperature mass spectrometric study of vaporization and thermodynamics of the Cs 2 O‐B 2 O 3 system: Review and experimental investigation

2021 ◽  
Vol 35 (11) ◽  
Author(s):  
Valentina L. Stolyarova ◽  
Viktor A. Vorozhtcov ◽  
Sergey I. Lopatin ◽  
Sergey M. Shugurov ◽  
Elizaveta P. Simonenko ◽  
...  
Keyword(s):  
Experimental Investigation ◽  
High Temperature ◽  
Mass Spectrometric Study ◽  
Mass Spectrometric ◽  
Spectrometric Study ◽  
System Review

scholarly journals Development, Optimization and Validation of a Sustainable and Quantifiable Methodology for the Determination of 2,4,6-Trichloroanisole, 2,3,4,6-Tetrachloroanisole, 2,4,6-Tribromoanisole, Pentachloroanisole, 2-Methylisoborneole and Geosmin in Air

Processes ◽  
2021 ◽  
Vol 9 (9) ◽  
pp. 1571
Author(s):  
Patricia Jové ◽  
Marina Vives-Mestres ◽  
Raquel De Nadal ◽  
Maria Verdum
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
High Temperature ◽  
Thermal Desorption ◽  
Mass Spectrometric ◽  
Gas Chromatography Mass Spectrometry ◽  
Air Analysis ◽  
Good Linearity ◽  
Repeatability And Reproducibility

Compounds 2,4,6-trichloroanisole (TCA), 2,3,4,6-tetrachloroanisole (TeCA), 2,4,6-tribromoanisole (TBA) and pentachloroanisole (PCA), 2-methylisoborneol (2MIB) and geosmin (GSM) have been reported as being responsible for cork and wine taint. A sustainable method based on thermal desorption-gas chromatography–mass spectrometry (TD-GC/MS) has been developed and optimized, taking into account desorption parameters and chromatographic and mass spectrometric conditions. The combination of parameters that jointly maximized the compound detection was as follows: desorption temperature at 300 °C, desorption time at 30 min, cryo-temperature at 20 °C and trap high temperature at 305 °C. The proposed methodology showed a good linearity (R ≤ 0.994) within the tested range (from 0.1 to 2 ng) for all target compounds. The precision expressed as repeatability and reproducibility was RSD < 10% in both. The limits of quantification ranged from 0.05 to 0.1 ng. The developed methodology and the sampling rates (R-values) of all targeted compounds (from 0.013 to 0.071 m3 h−1) were applied to the air analysis of two wineries. The results showed that the developed methodology is a sustainable and useful tool for the determination of these compounds in air.

Monodisperse single nanodiamond particulates

2008 ◽  
Vol 80 (7) ◽  
pp. 1365-1379 ◽  
Author(s):  
Eiji Ōsawa
Keyword(s):  
High Temperature ◽  
Sol Gel ◽  
Turn Of The Century ◽  
Detonation Nanodiamond ◽  
Wet Milling ◽  
Bottom Up ◽  
High Temperature And Pressure ◽  
Primary Particles ◽  
New Form ◽  
Temperature And Pressure

Detonation nanodiamond (DN) was discovered in 1963, but for several reasons was known only among a small number of scientists until the turn of the century. The most serious cause was the fact that primary nanocarbon particles formed by the "bottom-up method" are in general covalently bound together under high-temperature and -pressure conditions to form large agglutinates, which were difficult to separate by conventional methods. DN was not an exception. A breakthrough led to the isolation of primary particles having the expected size of 4-5 nm by wet-milling with zirconia micro-beads. Thus, long-waited primary particles of DN finally became available in kg quantities in the form of colloidal sol, gel, and readily redispersible flakes. Progress in the development of a new form of the old material is presented.

MASS SPECTROMETRIC STUDIES OF HIGH TEMPERATURE SYSTEMS

1963 ◽  
pp. 99-109 ◽  
Author(s):  
W.A. CHUPKA ◽  
J. BERKOWITZ ◽  
D.J. MESCHI ◽  
H.A. TASMAN
Keyword(s):  
High Temperature ◽  
Mass Spectrometric

Activity measurements on the Al-rich region of the Ni–Al system – A high temperature mass spectrometric study

2006 ◽  
Vol 97 (4) ◽  
pp. 461-470
Author(s):  
Tiruppatur Subramaniam Lakshmi Narasimhan ◽  
Dieter Kath ◽  
Torsten Markus ◽  
Klaus Hilpert
Keyword(s):  
High Temperature ◽  
Mass Spectrometric Study ◽  
Mass Spectrometric ◽  
Spectrometric Study ◽  
Rich Region ◽  
System A ◽  
Activity Measurements
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