A rapid and highly sensitive fluorimetric method for the determination of meloxicam using uranyl acetate

2014 ◽  
Vol 6 (14) ◽  
pp. 5221-5226 ◽  
Author(s):  
Jing Tian ◽  
Chunyan Li ◽  
Shaopu Liu ◽  
Zhongfang Liu ◽  
Jidong Yang ◽  
...  
Keyword(s):  
High Sensitivity ◽  
Uranyl Acetate ◽  
Fluorimetric Method ◽  
Spectrofluorimetric Method ◽  
Highly Sensitive

A novel quick spectrofluorimetric method with high sensitivity and simplicity for determining meloxicam was established based on the interaction of uranyl acetate with meloxicam.

Fluorimetric Method for the Determination of Trace Amounts of Molybdenum Using 2-Hydroxy-1-Naphthaldehydene-p-Aminotoluene

2014 ◽  
Vol 556-562 ◽  
pp. 584-587 ◽  
Author(s):  
Wei Wei Bian
Keyword(s):  
Detection Limit ◽  
Cation Exchange ◽  
Good Precision ◽  
Pig Liver ◽  
Fluorimetric Method ◽  
Ethanol Medium ◽  
Spectrofluorimetric Method ◽  
Highly Sensitive ◽  
Precision And Accuracy

A new fluorescent reagent, 2-hydroxy-1-naphthaldehydene-p-aminotoluene, was synthesized. The fluorescent reaction of this reagent with molybdenum was studied. Based on this chelation, a highly sensitive spectrofluorimetric method was developed for determination of trace amounts of molybdenum in a water-ethanol medium at pH 6.8. Under these conditions, the Mo-HNA complex has excitation and emission maxima at 300 and 380 nm, respectively. The linear range of the method is 0-2μg/L and the detection limit is 0.62μg/L. Interference of other ions was studied. It is necessary to remove the interfering cations through cation exchange. The procedure can be carried out easily and affords good precision and accuracy. This method has been successfully applied to the determination of molybdenum in pig liver and mussels.

scholarly journals New Spectrofluorimetric Method with Enhanced Sensitivity for Determination of Paroxetine in Dosage Forms and Plasma

2008 ◽  
Vol 3 ◽  
pp. ACI.S1053 ◽  
Author(s):  
Ibrahim A. Darwish ◽  
Sawsan M. Amer ◽  
Heba H. Abdine ◽  
Lama I. Al-Rayes
Keyword(s):  
High Sensitivity ◽  
Dosage Forms ◽  
Official Method ◽  
Factors Affecting ◽  
Pharmaceutical Tablets ◽  
Enhanced Sensitivity ◽  
Spectrofluorimetric Method ◽  
Relative Standard ◽  
Optimized Conditions

New simple spectrofluorimetric method with enhanced sensitivity has been developed and validated for the determination of the antidepressant paroxetine (PXT) in its dosage forms and plasma. The method was based on nucleophilic substitution reaction of PXT with 4-chloro-7-nitrobenzo-2-oxa-1,3-diazole in an alkaline medium (pH 8) to form a highly fluorescent derivative that was measured at 545 nm after excitation at 490 nm. The factors affecting the reaction was carefully studied and optimized. The kinetics of the reaction was investigated, and the reaction mechanism was presented. Under the optimized conditions, linear relationship with good correlation coefficient (0.9993) was found between the fluorescence intensity and PXT concentration in the range of 80-800 ng ml-1. The limits of detection and quantitation for the method were 25 and 77 ng ml-1, respectively. The precision of the method was satisfactory; the values of relative standard deviations did not exceed 3%. The proposed method was successfully applied to the determination of PXT in its pharmaceutical tablets with good accuracy; the recovery values were 100.2 ± 1.61%. The results obtained by the proposed method were comparable with those obtained by the official method. The proposed method is superior to the previously reported spectrofluorimetric method for determination of PXT in terms of its higher sensitivity and wider linear range. The high sensitivity of the method allowed its successful application to the analysis of PXT in spiked human plasma. The proposed method is practical and valuable for its routine application in quality control and clinical laboratories for analysis of PXT.

scholarly journals Determination of berberine hydrochloride using a fluorimetric method with silica nanoparticles as a probe

RSC Advances ◽  
2018 ◽  
Vol 8 (11) ◽  
pp. 6075-6082 ◽  
Author(s):  
Qing Liu ◽  
Zhihai Xie ◽  
Tao Liu ◽  
Jin Fan
Keyword(s):  
Aqueous Solution ◽  
Silica Nanoparticles ◽  
Berberine Hydrochloride ◽  
Fluorimetric Method ◽  
Spectrofluorimetric Method

A simple, green and sensitive spectrofluorimetric method was proposed for the determination of berberine hydrochloride (BRH) in aqueous solution.

A Highly Sensitive and Selective Spectrofluorimetric Method for the Determination of Nitrite in Food Products

2015 ◽  
Vol 9 (5) ◽  
pp. 1293-1300 ◽  
Author(s):  
Qiuhua Wang ◽  
Haiwei Huang ◽  
Baoming Ning ◽  
Minfeng Li ◽  
Lan He
Keyword(s):  
Food Products ◽  
Spectrofluorimetric Method ◽  
Highly Sensitive

Highly Sensitive Determination of Dopamine Using Osmium/Nafion Modified Disposable Integrated Biosensor

2009 ◽  
Vol 60-61 ◽  
pp. 311-314 ◽  
Author(s):  
Chun Xiu Liu ◽  
Hong Min Liu ◽  
Qing De Yang ◽  
Nan Sen Lin ◽  
Yi Lin Song ◽  
...  
Keyword(s):  
Detection Limit ◽  
Correlation Coefficient ◽  
Gold Electrode ◽  
High Sensitivity ◽  
Differential Pulse ◽  
Redox Polymer ◽  
Highly Sensitive ◽  
Sensitive Determination ◽  
Excellent Reproducibility

A biosensor based on gold electrode modified by Pt nanaoparticles/Osmium redox polymer/Nafion trilayer film was fabricated and used for selective and sensitive determination of dopamine. The biosensor is explored for DA sensing using the cyclic voltammetry (CV), amperometric and differential pulse voltammetric (DPV) methods. The CV anodic peak currents showed a linear range with a correlation coefficient of 0.996, localized in the concentration range 0~192 μM. The differential pulse voltammetric (DPV) peak currents were linear with DA concentration during 2~425 μM with correlation coefficient of 0.99. The biosensor showed high sensitivity of 0.5 nA /nM cm2 and excellent reproducibility with the detection limit of ~10 nM (S/N=3) for the determination of DA. The easy fabrication, low detection limit and high sensitivity of the integrated biosensor making it particularly suitable for the analytical purposes.

A QCM Immunosensor for Rapid Determination of Ractopamine in Food Based on Enzyme-AuNPs for Signal Amplification and Magnetic β-Cyclodextrins for Extraction

2015 ◽  
Vol 738-739 ◽  
pp. 56-60
Author(s):  
Shu Xian Chen ◽  
Jing Liu ◽  
Dao Dong Pan ◽  
Ning Gan
Keyword(s):  
Signal Amplification ◽  
Rapid Determination ◽  
Screening Method ◽  
High Sensitivity ◽  
The Novel ◽  
Highly Sensitive ◽  
Reliable Quantification ◽  
Highly Sensitive Detection ◽  
Amplification Strategy

An enzyme amplified immunosensor for highly sensitive detection of Ractopamine (RAC) in foodstuff was developed based on quartz crystal microbalance (QCM). The high sensitivity was achieved by enzyme-AuNPs signal amplification strategy and magnetic β-cyclodextrins (β-CD) enrichment capacity. The novel QCM immunosensor which combines with the advantages of high selectivity of immunoassays and the high sensitivity of QCM has been developed for the determination of trace residues of RAC in food production. Under optimum conditions, the differences in the frequencies (∆f) of the QCM were proportional to the concentration of RAC over the range from 0.01 to 10 ng mL-1. The minimal detection limit was 0.01 ng mL-1. Due to its high sensitivity, acceptable stability and good selectivity, the immunosensor realized reliable quantification of RAC in real foodstuff. The proposed project has the potential to become a successful on-site screening method in food safety.

A spectrofluorimetric method for the determination of acitretin in pharmaceuticals

2009 ◽  
Vol 63 (4) ◽  
Author(s):  
Suling Feng ◽  
Yi Zhang ◽  
Jing Fan
Keyword(s):  
Fluorescence Spectra ◽  
Hplc Method ◽  
Relative Fluorescence Intensity ◽  
Optimum Conditions ◽  
Green Fluorescence ◽  
Spectrofluorimetric Method ◽  
Highly Sensitive ◽  
Relative Standard ◽  
Effects Of Ph

AbstractA simple, rapid, and highly sensitive spectrofluorimetric method for the determination of acitretin was developed based on the strong green fluorescence of acitretin. Influence of organic solvents on the fluorescence spectra of acitretin was studied. Effects of pH, standing time, and foreign ions on the determination of acitretin were also examined. Under the optimum conditions, linear relationship between the relative fluorescence intensity and the concentration of acitretin in the range of 30.0–1100 ng mL−1 was obtained. Detection limit of this method is 9.56 ng mL−1 for acitretin. Relative standard deviation for the determination of 480 ng mL−1 of acitretin was 1.70 %. This method was used for the determination of acitretin in pharmaceuticals and the results were compared with those obtained by the HPLC method.

scholarly journals Second Derivative Synchronous Fluorescence Spectroscopy for the Simultaneous Determination of Metoclopramide and Pyridoxine in Syrup and Human Plasma

2008 ◽  
Vol 91 (3) ◽  
pp. 542-550 ◽  
Author(s):  
Nahed El-Enany
Keyword(s):  
Simultaneous Determination ◽  
Human Plasma ◽  
High Sensitivity ◽  
Second Derivative ◽  
Native Fluorescence ◽  
Highly Sensitive ◽  
Experimental Parameters ◽  
The Mean ◽  
Good Agreement

Abstract A rapid, simple, and highly sensitive second derivative synchronous fluorometric method has been developed for the simultaneous determination of metoclopramide (MT) and pyridoxine (PY) in a binary mixture. The method is based on measurement of the native fluorescence of these drugs at = 80 nm in methanol. The different experimental parameters affecting the native fluorescence of the drugs were carefully studied and optimized. The fluorescence-concentration plots were rectilinear over the ranges of 0.020.4 and 0.12 g/mL for MT and PY, respectively. The limits of detection were 0.003 and 0.007 g/mL and the limits of quantification were 0.008 and 0.02 g/mL for MT and PY, respectively. The proposed method was successfully applied to the determination of MT and PY in synthetic mixtures and in commercial syrup. The results were in good agreement with those obtained with a reported method. The high sensitivity attained by the proposed method allowed the determination of MT in spiked and real human plasma samples. The mean percent recoveries of MT from spiked and real human plasma (n = 3) were 93.72 3.15 and 89.72 2.19, respectively.

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