Analysis of six bioactive components in Semen Ziziphi Spinosae by UPLC-ELSD and UPLC-Q/TOF-MS

2014 ◽  
Vol 6 (15) ◽  
pp. 5856-5864 ◽  
Author(s):  
Xiangsheng Zhao ◽  
Liwei Xie ◽  
Haifeng Wu ◽  
Weijun Kong ◽  
Meihua Yang
Keyword(s):  
Analytical Method ◽  
Ultrasonic Extraction ◽  
Bioactive Components ◽  
Tof Ms

A fast and validated multicomponent analytical method was developed based on ultrasonic extraction, detection by UPLC-ELSD and confirmation by UPLC-Q/TOF-MS.

scholarly journals Rapid identification of direct-acting pancreatic protectants from Cyclocarya paliurus leaves tea by the method of serum pharmacochemistry combined with target cell extraction

2020 ◽  
Vol 18 (1) ◽  
pp. 265-274
Author(s):  
Wei-hong Chen ◽  
Zhen Luo ◽  
Zi-Wan Ning ◽  
Jiao Peng ◽  
Xiao-peng Hu ◽  
...  
Keyword(s):  
Target Cell ◽  
Rapid Identification ◽  
Target Cells ◽  
Bioactive Components ◽  
Β Cell ◽  
Cyclocarya Paliurus ◽  
Cell Extraction ◽  
Direct Acting ◽  
Apoptotic Effects ◽  
Tof Ms

AbstractExtracts of Cyclocarya paliurus (CP) leaves, a popular sweet tea, inhibit pancreatic β cell apoptosis and have potent hypoglycemic effects, but the identities of the anti-apoptotic bioactive components are still unknown. In the present study, a method using UPLC-Q-TOF/MS based on serum pharmacochemistry combined with target cell extraction was established to rapidly identify direct-acting pancreatic protectants from CP. After orally administering a set amount of CP extract to rats, blood samples were collected to characterize the components that can be absorbed into the blood using UPLC-Q-TOF/MS. Also, target cells (pancreatic β NIT-1 cells) were incubated with CP extract for 24 hours, and cells were collected to identify the components that can bind to the cells using UPLC-Q-TOF/MS. Finally, to evaluate the protective effect of the bioactive components of CP, MTT and TUNEL assays were performed on treated NIT-1 cell induced by streptozotocin (STZ). Three potential direct-acting pancreatic protectants -- kaempferol, quercetin, quadranoside IV -- were identified, and anti-apoptotic effects of kaempferol and quercetin were confirmed in STZ-induced NIT-1 cells. The findings indicate that this combined approach is a feasible, rapid, and expedient tool for capturing potential direct-acting components from natural products such as those from CP leaves.

New analytical method for the study of the metabolism of gentiopicroside in rats after oral administration by LC-TOF-MS following picolinoyl derivatization

2013 ◽  
Vol 37 (3) ◽  
pp. 237-243 ◽  
Author(s):  
Zhigang Wang ◽  
Shanshan Wang ◽  
Yujia Sun ◽  
Huiyu Wang ◽  
Guang Chen ◽  
...  
Keyword(s):  
Oral Administration ◽  
Analytical Method ◽  
Tof Ms

New analytical method for the study of metabolism of swertiamarin in rats after oral administration by UPLC-TOF-MS following DNPH derivatization

2015 ◽  
Vol 29 (8) ◽  
pp. 1184-1189 ◽  
Author(s):  
Xiuhong Wu ◽  
Shuhan Tang ◽  
Yan Jin ◽  
Yan Zhang ◽  
Masao Hattori ◽  
...  
Keyword(s):  
Oral Administration ◽  
Analytical Method ◽  
Dnph Derivatization ◽  
Tof Ms
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