Partition of proteins with extraction in aqueous two-phase system by hydroxyl ammonium-based ionic liquid

2014 ◽  
Vol 6 (12) ◽  
pp. 4067-4076 ◽  
Author(s):  
Jing Chen ◽  
Yuzhi Wang ◽  
Qun Zeng ◽  
Xueqin Ding ◽  
Yanhua Huang
Keyword(s):  
Ionic Liquid ◽  
Ionic Liquids ◽  
Phase System ◽  
Two Phase ◽  
Aqueous Two Phase System ◽  
Ammonium Ionic Liquids

A series of hydroxyl ammonium ionic liquids have been designed and synthesized and used for the extraction of proteins.

Partitioning Behavior of Penicillin G in Aqueous Two Phase System Based on Ionic Liquids

2013 ◽  
Vol 864-867 ◽  
pp. 324-327
Author(s):  
Bin Jiang ◽  
Zhi Biao Feng ◽  
Chun Hong Liu ◽  
Ying Cao Xu ◽  
Dong Mei Li ◽  
...  
Keyword(s):  
Ionic Liquid ◽  
Ionic Liquids ◽  
Extraction Yield ◽  
Penicillin G ◽  
Phase System ◽  
Two Phase ◽  
Aqueous Two Phase System ◽  
Partitioning Behavior

An aqueous two-phase system (ATPS) was presented with hydrophilic ionic liquid 1-Butyl-3-methylimid-azolium tetrafluoroborate ([BmiBF4) and salts for the extraction of penicillin G in this paper. The phase forming abilities of different salts were investigated to choose a suitable salt.The partitioning behavior of penicillin G in the ATPS was investigated.Concentrations of (NH4)2SO4, penicillin G, and [BmiBF4 were evaluated to determine their effects on the extraction yield of penicillin G. It was found that extraction yield strongly depended on the concentration of [BmiBF4, penicillin and (NH4)2SO4. A high extraction yield around 93% was achieved with the following parameters: (NH4)2SO4 20% (wt%), penicillin 40000 ~ 45000 U/mL, [BmiBF4 20% (wt%).

Chiral separation of α-cyclohexylmandelic acid enantiomers using ionic liquid/salt aqueous two-phase system

2015 ◽  
Vol 69 (11) ◽  
Author(s):  
Lei-Lei Chen ◽  
Fen-Fang Li ◽  
Zhi-Jian Tan
Keyword(s):  
Ionic Liquid ◽  
Ionic Liquids ◽  
Chiral Separation ◽  
Mixing Time ◽  
Chiral Selector ◽  
Phase System ◽  
Two Phase ◽  
Aqueous Two Phase System ◽  
Liquid Salt ◽  
Influencing Parameters

AbstractIn this paper, an ionic liquid-based aqueous two-phase system (ILATPS) was applied to the chiral separation of α-cyclohexylmandelic acid (α-CHMA) enantiomers with hydroxypropyl-β- cyclodextrin (HP-β-CD) as the chiral selector. Several influencing parameters were investigated including the types and concentration of ionic liquids, the amount of phase-forming salt, temperature, mixing time, pH, and the content of HP-β-CD. The results showed that not all ILATPS had the ability to chirally recognise the selected enantiomers and that [C

scholarly journals Extraction of Lysozyme by Hydroxyl-Functionalized Ionic Liquid Aqueous Two-Phase System

2015 ◽  
Vol 27 (8) ◽  
pp. 2759-2762 ◽  
Author(s):  
Bo Zhou ◽  
Gaozhang Gou ◽  
Shijuan Xu ◽  
Pei Zhang ◽  
Wei Liu
Keyword(s):  
Ionic Liquid ◽  
Phase System ◽  
Two Phase ◽  
Aqueous Two Phase System ◽  
Functionalized Ionic Liquid
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