Determination of multi-residue PCBs in air by real-time fluorescent quantitative immuno-PCR assay

2014 ◽  
Vol 6 (17) ◽  
pp. 6925-6930 ◽  
Author(s):  
Han-Yu Chen ◽  
Hui-Sheng Zhuang ◽  
Guang-Xin Yang
Keyword(s):  
Real Time ◽  
Fluorescence Signal ◽  
High Concentration ◽  
Pcr Assay ◽  
Low Concentration ◽  
Dilution Series ◽  
Aroclor 1248 ◽  
Direct Competition

Amplification curves of a dilution series of Aroclor 1248 of direct competition rt-IPCR. In the figure, the fluorescence signal of the curve (10 fg mL−1) reaching the threshold was at around cycle 12.3, and there was a fall in the Ct value from 10 to 106 fg mL−1. This result implied that the time expended to reach the threshold for the high concentration of PCB molecules was much longer than for the low concentration PCBs.

Determination of phenanthrene by antibody-coated competitive real-time immuno-PCR assay

2008 ◽  
Vol 391 (8) ◽  
pp. 2857-2863 ◽  
Author(s):  
Chun Zhou ◽  
Qiong-E Wang ◽  
Hui-Sheng Zhuang
Keyword(s):  
Real Time ◽  
Pcr Assay

Real-time PCR-assay in the delivery suite for determination of group B streptococcal colonization in a setting with risk-based antibiotic prophylaxis

2013 ◽  
Vol 27 (4) ◽  
pp. 328-332 ◽  
Author(s):  
Stellan Håkansson ◽  
Karin Källén ◽  
Maria Bullarbo ◽  
Per-Åke Holmgren ◽  
Katarina Bremme ◽  
...  
Keyword(s):  
Antibiotic Prophylaxis ◽  
Real Time ◽  
Real Time Pcr ◽  
Pcr Assay ◽  
Group B

scholarly journals Determination of RhD Zygosity: Comparison of a Double Amplification Refractory Mutation System Approach and a Multiplex Real-Time Quantitative PCR Approach

2001 ◽  
Vol 47 (4) ◽  
pp. 667-672 ◽  
Author(s):  
Rossa W K Chiu ◽  
Michael F Murphy ◽  
Carrie Fidler ◽  
Benny C Y Zee ◽  
James S Wainscoat ◽  
...  
Keyword(s):  
Real Time ◽  
Quantitative Pcr ◽  
Gene Dosage ◽  
Amplification Refractory Mutation System ◽  
Pcr Assay ◽  
Real Time Quantitative Pcr ◽  
Mutation System ◽  
Allele Specific ◽  
Quantitative Pcr Assay

Abstract Background: Rh isoimmunization and hemolytic disease of the newborn still occur despite the availability of Rh immunoglobulin. For the prenatal investigation of sensitized RhD-negative pregnant women, determination of the zygosity of the RhD-positive father has important implications. The currently available molecular methods for RhD zygosity assessment, in general, are technically demanding and labor-intensive. Therefore, at present, rhesus genotype assessment is most commonly inferred from results of serological tests. The recent elucidation of the genetic structure of the prevalent RHD deletion in Caucasians, as well as the development of real-time PCR, allowed us to explore two new approaches for the molecular determination of RhD zygosity. Methods: Two methods for RhD zygosity determination were developed. The first was based on the double Amplification Refractory Mutation System (double ARMS). The second was based on multiplex real-time quantitative PCR. For the double ARMS assay, allele-specific primers were designed to directly amplify the most prevalent RHD deletion found in RhD-negative individuals in the Caucasian population. The multiplex real-time quantitative PCR assay, on the other hand, involved coamplification and quantification of RHD-specific sequences in relation to a reference gene, albumin, in a single PCR reaction. A ratio, ΔCt, based on the threshold cycle, was then determined and reflects the RHD gene dosage. Results: The allele-specific primers of the double ARMS assay reliably amplified the RHD-deleted allele and therefore accurately distinguished homozygous from heterozygous RhD-positive samples. The results were in complete concordance with serological testing. For the multiplex real-time quantitative PCR assay, the ΔCt values clearly segregated into two distinct populations according to the RHD gene dosage, with mean values of 1.70 (SD, 0.17) and 2.62 (SD, 0.29) for the homozygous and heterozygous samples, respectively (P <0.001, t-test). The results were in complete concordance with the results of serological testing as well as with the double ARMS assay. Conclusion: Double ARMS and real-time quantitative PCR are alternative robust assays for the determination of RhD zygosity.

scholarly journals Determination of Sex Ratio In Bovine Semen Using SYBR Green Real-Time PCR

2022 ◽  
Author(s):  
Vemula Harshini ◽  
S.M.K. Karthickeyan ◽  
K.G P. Kumarasamy ◽  
Tirumurugaan ◽  
C. Jeevan
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Sybr Green ◽  
Pcr Assay ◽  
Mean Values ◽  
Bovine Semen ◽  
Routine Basis ◽  
Repeatability And Reproducibility ◽  
The Mean

Abstract A SYBR green real-time PCR assay was developed to find out the sex skewness in bovine sex-sorted semen samples. The qPCR assay of PLP and SRY genes revealed the mean values of X- and Y-bearing spermatozoa as 50.24 ± 0.65 and 49.75 ± 0.62 per cent in unsorted, and 91.80 ± 0.79 and 8.20 ± 0.73 per cent in X-enriched semen samples respectively.. The amplification efficiencies of the PLP and SRY primers were 99.25 and 98.03 per cent respectively. The method was validated by a series of repeatability and reproducibility assays which revealed low co-efficients of variations as 2.19 and 3.12 per cent respectively Thus becoming a reliable and inexpensive tool to evaluate the sorted semen on routine basis and validation of other sperm sexing technologies.

A real-time immuno-PCR assay for the detection of tetrabromobisphenol A

2015 ◽  
Vol 7 (1) ◽  
pp. 99-106 ◽  
Author(s):  
Dan Bu ◽  
Huisheng Zhuang ◽  
Guangxin Yang ◽  
Xianyin Ping
Keyword(s):  
Real Time ◽  
Tetrabromobisphenol A ◽  
Pcr Assay

In this study, a reliable and ultra-sensitive indirect competitive real-time immuno-PCR (rt-iPCR) was established for the determination of tetrabromobisphenol A (TBBPA).

scholarly journals Single Monochrome Real-Time RT-PCR Assay for Identification, Quantification, and Breakpoint Cluster Region Determination of t(9;22) Transcripts

2005 ◽  
Vol 7 (1) ◽  
pp. 40-47 ◽  
Author(s):  
Marina I. Gutiérrez ◽  
Georgina Timson ◽  
Abdul K. Siraj ◽  
Rong Bu ◽  
Shakuntala Barbhaya ◽  
...  
Keyword(s):  
Real Time ◽  
Rt Pcr ◽  
Pcr Assay ◽  
Breakpoint Cluster Region ◽  
Cluster Region
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