Hydride-based HPLC stationary phases: a rapidly evolving technology for the development of new bio-analytical methods

2014 ◽  
Vol 6 (13) ◽  
pp. 4496-4503 ◽  
Author(s):  
Joseph J. Pesek ◽  
Reinhard I. Boysen ◽  
Milton T. W. Hearn ◽  
Maria T. Matyska
Keyword(s):  
Analytical Methods ◽  
Stationary Phases ◽  
Reversed Phase ◽  
Normal Phase ◽  
Silica Hydride ◽  
Phase Selectivity ◽  
Aqueous Normal Phase

This review focuses on the application of various silica hydride stationary phases under conditions that enable the advantages of reversed-phase, aqueous normal phase and organic normal phase selectivity to be gained.

Aqueous normal-phase chromatography using silica-hydride-based stationary phases

2013 ◽  
Vol 42 ◽  
pp. 64-73 ◽  
Author(s):  
Joseph J. Pesek ◽  
Maria T. Matyska ◽  
Reinhard I. Boysen ◽  
Yuanzhong Yang ◽  
Milton T.W. Hearn
Keyword(s):  
Stationary Phases ◽  
Normal Phase ◽  
Silica Hydride ◽  
Aqueous Normal Phase ◽  
Normal Phase Chromatography

Simultaneous separation of hydrophobic and hydrophilic peptides with a silica hydride stationary phase using aqueous normal phase conditions

2011 ◽  
Vol 1218 (44) ◽  
pp. 8021-8026 ◽  
Author(s):  
Reinhard I. Boysen ◽  
Yuanzhong Yang ◽  
Jamil Chowdhury ◽  
Maria T. Matyska ◽  
Joseph J. Pesek ◽  
...  
Keyword(s):  
Stationary Phase ◽  
Normal Phase ◽  
Simultaneous Separation ◽  
Silica Hydride ◽  
Aqueous Normal Phase

scholarly journals The Separation of Cannabinoids on Sub-2 µm Immobilized Polysaccharide Chiral Stationary Phases

2021 ◽  
Vol 14 (12) ◽  
pp. 1250
Author(s):  
Takafumi Onishi ◽  
Weston J. Umstead
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
High Performance ◽  
Stationary Phases ◽  
Chiral Stationary Phases ◽  
Reversed Phase ◽  
Normal Phase ◽  
Elution Order ◽  
Testing Methods ◽  
Structural Isomers

The increased use and applicability of Cannabis and Cannabis-derived products has skyrocketed over the last 5 years. With more and more governing bodies moving toward medical and recreational legalization, the need for robust and reliable analytical testing methods is also growing. While many stationary phases and methods have been developed for this sort of analysis, chiral stationary phases (CSPs) are unique in this area; not only can they serve their traditional chiral separation role, but they can also be used to perform achiral separations. Given that mixtures of cannabinoids routinely contain enantiomers, diastereomers, and structural isomers, this offers an advantage over the strictly achiral-only analyses. This work presents the separation of a 10-cannabinoid mixture on several polysaccharide-based sub-2 µm CSPs with both normal-phase and reversed-phase ultra-high-performance liquid chromatography (UHPLC) conditions. Along with the separation of the mixture, appropriate single-peak identification was performed to determine the elution order and reported where applicable.

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