scholarly journals Rapid biodiagnostic ex vivo imaging at 1 μm pixel resolution with thermal source FTIR FPA

The Analyst ◽  
2015 ◽  
Vol 140 (7) ◽  
pp. 2493-2503 ◽  
Author(s):  
C. R. Findlay ◽  
R. Wiens ◽  
M. Rak ◽  
J. Sedlmair ◽  
C. J. Hirschmugl ◽  
...  

Novel high spatial resolution (1 × 1 μm pixel) FTIR imaging with commercial benchtop instrument yields data comparable to that from synchrotron sources.

Coral Reefs ◽  
2021 ◽  
Author(s):  
E. Casoli ◽  
D. Ventura ◽  
G. Mancini ◽  
D. S. Pace ◽  
A. Belluscio ◽  
...  

AbstractCoralligenous reefs are characterized by large bathymetric and spatial distribution, as well as heterogeneity; in shallow environments, they develop mainly on vertical and sub-vertical rocky walls. Mainly diver-based techniques are carried out to gain detailed information on such habitats. Here, we propose a non-destructive and multi-purpose photo mosaicking method to study and monitor coralligenous reefs developing on vertical walls. High-pixel resolution images using three different commercial cameras were acquired on a 10 m2 reef, to compare the effectiveness of photomosaic method to the traditional photoquadrats technique in quantifying the coralligenous assemblage. Results showed very high spatial resolution and accuracy among the photomosaic acquired with different cameras and no significant differences with photoquadrats in assessing the assemblage composition. Despite the large difference in costs of each recording apparatus, little differences emerged from the assemblage characterization: through the analysis of the three photomosaics twelve taxa/morphological categories covered 97–99% of the sampled surface. Photo mosaicking represents a low-cost method that minimizes the time spent underwater by divers and capable of providing new opportunities for further studies on shallow coralligenous reefs.


The Analyst ◽  
2017 ◽  
Vol 142 (1) ◽  
pp. 156-168 ◽  
Author(s):  
Artur Dawid Surowka ◽  
Michael Pilling ◽  
Alex Henderson ◽  
Herve Boutin ◽  
Lidan Christie ◽  
...  

High spatial resolution FTIR imaging of early-stage 3-Tg-APP-PSP1-TAU mouse brain identifies molecular burden around Aβ deposits.


2021 ◽  
Vol 76 ◽  
pp. 39-48
Author(s):  
Sanghoon Kim ◽  
Ken Sakaie ◽  
Ingmar Blümcke ◽  
Stephen Jones ◽  
Mark J. Lowe

2020 ◽  
Author(s):  
Francesco Massimetti ◽  
Diego Coppola ◽  
Marco Laiolo ◽  
Sébastien Valade ◽  
Corrado Cigolini ◽  
...  

<p>In the satellite thermal remote sensing, the high-spatial resolution sensors may improve thermal constraining of volcanic phenomena, with direct implications on the comprehension of volcanic processes and monitoring purposes. Here we present a new hot-spot detection algorithm, developed for SENTINEL 2 (S2) data, which combines contextual spectral and spatial analysis, applied on the 8a-11-12 SWIR bands with 20 meters/pixel resolution. The algorithm is able to detect and count the number of hotspot-contaminated pixels (S2Pix), in a wide range of environments and for several types of volcanic activities. The S2-derived thermal trends, retrieved at different worldwide key-cases volcanoes, are than compared with the Volcanic Radiative Power (VRP) from MODIS images processed by the MIROVA system during the period 2016-2019. Dataseries showed an overall excellent correlation between the two imagery suites, enhancing the higher sensitivity of SENTINEL-2 to detect small size and subtle, low-temperature thermal signals. Results outline a relation between the S2Pix and VRP ratios and the volcanic processes (i.e. lava flows, domes, lakes, open-vent activity) producing a distinct pattern in terms of size and intensity of the thermal anomaly. Moreover, the high-spatial resolution of S2 imagery potentiality let to decrypt which is the thermal contribution of the different active volcanic portions, and to understand their evolution in terms of intensity and persistence. Our analysis indicates how the combination of high- (S2) and moderate- (MODIS) resolution thermal timeseries represent an improvement in the space-based volcano monitoring that can be useful for monitoring applications and communities which relate with active volcanoes.</p>


2012 ◽  
Vol 302 (1) ◽  
pp. H287-H298 ◽  
Author(s):  
Stephen H. Gilbert ◽  
David Benoist ◽  
Alan P. Benson ◽  
Ed White ◽  
Steven F. Tanner ◽  
...  

It has been shown by histology that cardiac myocytes are organized into laminae and this structure is important in function, both influencing the spread of electrical activation and enabling myocardial thickening in systole by laminar sliding. We have carried out high-spatial resolution three-dimensional MRI of the ventricular myolaminae of the entire volume of the isolated rat heart after contrast perfusion [dimeglumine gadopentate (Gd-DTPA)]. Four ex vivo rat hearts were perfused with Gd-DTPA and fixative and high-spatial resolution MRI was performed on a 9.4T MRI system. After MRI, cryosectioning followed by histology was performed. Images from MRI and histology were aligned, described, and quantitatively compared. In the three-dimensional MR images we directly show the presence of laminae and demonstrate that these are highly branching and are absent from much of the subepicardium. We visualized these MRI volumes to demonstrate laminar architecture and quantitatively demonstrated that the structural features observed are similar to those imaged in histology. We showed qualitatively and quantitatively that laminar architecture is similar in the four hearts. MRI can be used to image the laminar architecture of ex vivo hearts in three dimensions, and the images produced are qualitatively and quantitatively comparable with histology. We have demonstrated in the rat that: 1) laminar architecture is consistent between hearts; 2) myolaminae are absent from much of the subepicardium; and 3) although localized orthotropy is present throughout the myocardium, tracked myolaminae are branching structures and do not have a discrete identity.


2021 ◽  
Author(s):  
Gabriel Ramos-Llordén ◽  
Rodrigo Lobos ◽  
Tae Hyung Kim ◽  
Qiyuan Tian ◽  
Thomas Witzel ◽  
...  

Diffusion MRI (dMRI) of whole, intact, fixed postmortem human brain at high spatial resolution serves as key bridging technology for 3D mapping of structural connectivity and tissue microstructure at the mesoscopic scale. Ex vivo dMRI offers superior spatial resolution compared to in vivo dMRI but comes with its own technical challenges due to the significantly reduced T2 relaxation times and decreased diffusivity incurred by tissue fixation. The altered physical properties of fixed tissue necessitate the use of alternative acquisition strategies to preserve SNR and achieve sufficient diffusion weighting. Multi-shotor segmented 3D echo planar imaging (EPI) sequences have been used to shorten echo times (TEs) with reduced distortions from field inhomogeneity and eddy currents on small-bore MR scanners and have been adopted for high b-value dMRI of ex vivo whole human brain specimens. The advent of stronger gradients on human MRI scanners has led to improved image quality and a wider range of diffusion-encoding parameters for dMRI but at the cost of more severe eddy currents that result in spatial and temporal variations in the background magnetic field, which cannot be corrected for using standard vendor-provided ghost correction solutions. In this work, we show that conventional ghost correction techniques based on navigators and linear phase correction may be insufficient for EPI sequences using strong diffusion-sensitizing gradients in ex vivo dMRI experiments, resulting in orientationally biased dMRI estimates. This previously unreported problem is a critical roadblock in any effort to leverage scanners with ultra-high gradients for high-precision mapping of human neuroanatomy at the mesoscopic scale. We propose an advanced reconstruction method based on structured low-rank matrix modeling that reduces the ghosting substantially. We show that this method leads to more accurate and reliable dMRI metrics, as exemplified by diffusion tensor imaging and high angular diffusion imaging analyses in distributed neuroanatomical areas of fixed whole human brain specimens. Our findings advocate for the use of advanced reconstruction techniques for recovering unbiased metrics from ex vivo dMRI acquisitions and represent a crucial step toward making full use of strong diffusion-encoding gradients for neuroscientific studies seeking to study brain structure at multiple spatial scales.


2017 ◽  
Vol 56 (28) ◽  
pp. 7886 ◽  
Author(s):  
Tiffany C. Kwong ◽  
Farouk Nouizi ◽  
Jaedu Cho ◽  
Yuting Lin ◽  
Uma Sampathkumaran ◽  
...  

2017 ◽  
Vol 38 ◽  
pp. 174-181 ◽  
Author(s):  
Ichiro Yamada ◽  
Norio Yoshino ◽  
Keigo Hikishima ◽  
Naoyuki Miyasaka ◽  
Shinichi Yamauchi ◽  
...  

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