An instantaneous colorimetric protein assay based on spontaneous formation of a protein corona on gold nanoparticles

The Analyst ◽  
2015 ◽  
Vol 140 (4) ◽  
pp. 1026-1036 ◽  
Author(s):  
Yan Teck Ho ◽  
Barbara Poinard ◽  
Eugenia Li Ling Yeo ◽  
James Chen Yong Kah
Keyword(s):  
Gold Nanoparticles ◽  
Protein Corona ◽  
Protein Assay ◽  
Spontaneous Formation

A schematic of the NP-based protein assay.

Time evolution of β-lactoglobulin corona formation on gold nanoparticles and impact on allergy

2020 ◽  
Author(s):  
Xiaoning Zhang ◽  
Meifeng Li ◽  
Yuanping Lv ◽  
Xiaoling Sun ◽  
Yao Han ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Secondary Structure ◽  
Time Evolution ◽  
Electrostatic Force ◽  
Protein Corona ◽  
Raw Milk ◽  
Covalent Bonds ◽  
Corona Formation ◽  
Β Lactoglobulin ◽  
Β Sheet

Abstract Gold nanoparticles (AuNPs) are modified immediately by the adsorption of β-lactoglobulin (βlg) when designed as colorimetric probe in raw milk, leading to the formation of a protein corona. This adsorption results mainly from a fast electrostatic force and a slow formation of Au-S covalent bonds, which is a precondition for the use of AuNPs in biodetection. The proteins corona influences the structure and bioactivity of adsorbed protein, such as the allergy. In this study, the mechanism of βlg adsorbed on AuNPs was investigated in terms of stoichiometry, binding affinity (Ka), time evolution of Au-S bond, and general secondary structure changes to address the desensitization of AuNPs. The results show that about 3,600 βlg are adsorbed on a single AuNPs, and the Ka is 2.9 ± 0.7 × 10 6 M -1 . The formation of Au-S bonds takes about 9 h, which is the time needed for complete changes in secondary structure and the IgE combining capacity. The structure of allergenic epitopes assigned to β-sheet was destroyed by the formation of Au-S bond, then induced to the decrease allergy. Furthermore, Fourier transform infrared spectroscopy confirmed a decrease in β-sheet contents after conjugated with AuNPs.

scholarly journals A Critical Factor for Quantifying Proteins in Unmodified Gold Nanoparticles-Based Aptasensing: The Effect of pH

Chemosensors ◽  
2020 ◽  
Vol 8 (4) ◽  
pp. 98
Author(s):  
Dai Lu ◽  
Dong Zhang ◽  
Qian Zhao ◽  
Xiangyang Lu ◽  
Xingbo Shi
Keyword(s):  
Gold Nanoparticles ◽  
Limit Of Detection ◽  
Protein Detection ◽  
Critical Factor ◽  
Cost Effective ◽  
Protein Corona ◽  
Target Protein ◽  
Universal Method ◽  
Salt Addition ◽  
Positively Charged

Unmodified gold nanoparticles (AuNPs)-based aptasensing (uGA) assay has been widely implemented in the determination of many different targets, but there are few reports on protein detection using uGA. Here, we designed a uGA assay for protein detection including the elimination of interfering proteins. Positively charged protein can be absorbed directly on the surface of AuNPs to form “protein corona”, which results in the aggregation of AuNPs even without salt addition, thereby preventing target protein detection. To overcome this problem, we systematically investigated the effect of modifying the pH of the solution during the uGA assay. A probe solution with a pH slightly higher than the isoelectric points (pI) of the target protein was optimal for protein detection in the uGA assay, allowing the aptamer to selectively detect the target protein. Three proteins (beta-lactoglobulin, lactoferrin, and lysozyme) with different pI were chosen as model proteins to validate our method. Positively charged interfering proteins (with pIs higher than the optimal pH) were removed by centrifugation of protein corona/AuNPs aggregates before the implementation of actual sample detection. Most importantly, the limit of detection (LOD) for all three model proteins was comparable to that of other methods, indicating the significance of modulating the pH. Moreover, choosing a suitable pH for a particular target protein was validated as a universal method, which is significant for developing a novel, simple, cost-effective uGA assay for protein detection.

scholarly journals Spontaneous Formation of Functionalized Dendrimer-Stabilized Gold Nanoparticles

2008 ◽  
Vol 112 (22) ◽  
pp. 8251-8258 ◽  
Author(s):  
Xiangyang Shi ◽  
Kai Sun ◽  
James R. Baker
Keyword(s):  
Gold Nanoparticles ◽  
Spontaneous Formation

scholarly journals Computational Insight on the Interaction of Common Blood Proteins with Gold Nanoparticles

2021 ◽  
Vol 22 (16) ◽  
pp. 8722
Author(s):  
Francesco Tavanti ◽  
Maria Cristina Menziani
Keyword(s):  
Gold Nanoparticles ◽  
Adsorption Capacity ◽  
Protein Interactions ◽  
Hydrophobic Interactions ◽  
Protein Corona ◽  
Coarse Grained ◽  
Complement C3 ◽  
Maximum Adsorption Capacity ◽  
Blood Proteins ◽  
Biological Phenomena

Protein interactions with engineered gold nanoparticles (AuNPs) and the consequent formation of the protein corona are very relevant and poorly understood biological phenomena. The nanoparticle coverage affects protein binding modalities, and the adsorbed protein sites influence interactions with other macromolecules and cells. Here, we studied four common blood proteins, i.e., hemoglobin, serum albumin, α1-antiproteinase, and complement C3, interacting with AuNPs covered by hydrophobic 11-mercapto-1-undecanesulfonate (MUS). We use Molecular Dynamics and the Martini coarse−grained model to gain quantitative insight into the kinetics of the interaction, the physico-chemical characteristics of the binding site, and the nanoparticle adsorption capacity. Results show that proteins bind to MUS−capped AuNPs through strong hydrophobic interactions and that they adapt to the AuNP surfaces to maximize the contact surface, but no dramatic change in the secondary structure of the proteins is observed. We suggest a new method to calculate the maximum adsorption capacity of capped AuNPs based on the effective surface covered by each protein, which better represents the realistic behavior of these systems.

scholarly journals Gold Nanostars with Reduced Fouling Facilitate Small Molecule Detection in the Presence of Protein

Nanomaterials ◽  
2021 ◽  
Vol 11 (10) ◽  
pp. 2565
Author(s):  
Anastasiia Tukova ◽  
Inga Christine Kuschnerus ◽  
Alfonso Garcia-Bennett ◽  
Yuling Wang ◽  
Alison Rodger
Keyword(s):  
Gold Nanoparticles ◽  
Small Molecule ◽  
Protein Corona ◽  
Surface Enhanced Raman Spectroscopy ◽  
Biological Media ◽  
Gold Nanostars ◽  
Cellular Environment ◽  
Vis Spectroscopy ◽  
Corona Formation

Gold nanoparticles have the potential to be used in biomedical applications from diagnostics to drug delivery. However, interactions of gold nanoparticles with different biomolecules in the cellular environment result in the formation of a “protein corona”—a layer of protein formed around a nanoparticle, which induces changes in the properties of nanoparticles. In this work we developed methods to reproducibly synthesize spheroidal and star-shaped gold nanoparticles, and carried out a physico-chemical characterization of synthesized anionic gold nanospheroids and gold nanostars through transmission electron microscopy (TEM), dynamic light scattering (DLS), zeta potential (ZP), nanoparticles tracking analysis (NTA), ultraviolet-visible (UV–Vis) spectroscopy and estimates of surface-enhanced Raman spectroscopy (SERS) signal enhancement ability. We analyzed how they interact with proteins after pre-incubation with bovine serum albumin (BSA) via UV–Vis, DLS, ZP, NTA, SERS, cryogenic TEM (cryo-TEM) and circular dichroism (CD) spectroscopy. The tests demonstrated that the protein adsorption on the particles’ surfaces was different for spheroidal and star shaped particles. In our experiments, star shaped particles limited the protein corona formation at SERS “hot spots”. This benefits the small-molecule sensing of nanostars in biological media. This work adds more understanding about protein corona formation on gold nanoparticles of different shapes in biological media, and therefore guides design of particles for studies in vitro and in vivo.

scholarly journals Reversible Control of Protein Corona Formation on Gold Nanoparticles Using Host–Guest Interactions

ACS Nano ◽  
2020 ◽  
Vol 14 (5) ◽  
pp. 5382-5391 ◽  
Author(s):  
Jesús Mosquera ◽  
Isabel García ◽  
Malou Henriksen-Lacey ◽  
Miguel Martínez-Calvo ◽  
Mónica Dhanjani ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Protein Corona ◽  
Corona Formation

Protein Corona on Gold Nanoparticles Studied with Coarse-Grained Simulations

Langmuir ◽  
2020 ◽  
Vol 36 (44) ◽  
pp. 13356-13363
Author(s):  
Md Symon Jahan Sajib ◽  
Pranab Sarker ◽  
Yong Wei ◽  
Xiuping Tao ◽  
Tao Wei
Keyword(s):  
Gold Nanoparticles ◽  
Protein Corona ◽  
Coarse Grained ◽  
Coarse Grained Simulations
Sign in / Sign up

Export Citation Format

Share Document