Fluorescent silver nanocluster DNA probes for multiplexed detection using microfluidic capillary electrophoresis

The Analyst ◽  
2015 ◽  
Vol 140 (5) ◽  
pp. 1609-1615 ◽  
Author(s):  
Jackson Travis Del Bonis-O'Donnell ◽  
Deborah K. Fygenson ◽  
Sumita Pennathur
Keyword(s):  
Capillary Electrophoresis ◽  
Hepatitis A ◽  
Dna Probes ◽  
Multiplexed Detection ◽  
Silver Nanocluster ◽  
Electrophoretic Mobilities

We tune the electrophoretic mobilities of silver nanocluster DNA probes for Hepatitis A, B and C targets for single-color multiplexing by microfluidic capillary electrophoresis.

Control of relative migration of small inorganic and organic anions with cyclodextrins in capillary electrophoresis (CE)

1998 ◽  
Vol 76 (2) ◽  
pp. 194-198
Author(s):  
Costas Stathakis ◽  
Richard M Cassidy
Keyword(s):  
Capillary Electrophoresis ◽  
Electroosmotic Flow ◽  
Organic Anions ◽  
Sodium Chromate ◽  
Migration Patterns ◽  
Electrophoretic Mobilities ◽  
Potassium Hydrogen ◽  
Capillary Electrophoretic ◽  
Relative Migration ◽  
Inorganic And Organic

The capillary electrophoretic separation of iodide, nitrate, perchlorate, thiocyanate, bromate, iodate, and ethane-, butane-, pentane-, and octanesulphonate was examined in sodium chromate or potassium hydrogen phthalate electrolytes and in the presence of α -, γ -, (0-40 mmol/L) and β -cyclodextrin (0-10 mmol/L). Largest decreases in electrophoretic mobility were observed for iodide, perchlorate, and thiocyanate, probably due to inclusion of these anions in the cyclodextrin (CD) cavity. Changes in migration patterns and electroosmotic flow were observed, which depended on cyclodextrin type and concentration and on electrolyte-cyclodextrin interactions. Thus for larger cavity cyclodextrins ( β-or γ-cyclodextrin) or in the presence of chromate, relatively small decreases in electrophoretic mobilities for all the anions were observed, indicating that a good match between analyte and cavity size and minimal cyclodextrin affinity for electrolyte ions are essential for different migration patterns. Separation efficiencies were between 50 000 and 400 000 theoretical plates, and calibration plots for iodide and octanesulphonate were linear; R2 = 0.998 and 0.9999, respectively, in the concentration range (5 x 10-5)-(5 x 10-3) mol/L.Key words: capillary electrophoresis, inorganic and organic anions, cyclodextrins, inclusion complex, electroosmotic flow control.

Multiplexed Detection of Small Analytes by Structure-Switching Aptamer-Based Capillary Electrophoresis

2010 ◽  
Vol 82 (11) ◽  
pp. 4613-4620 ◽  
Author(s):  
Zhenyu Zhu ◽  
Corinne Ravelet ◽  
Sandrine Perrier ◽  
Valérie Guieu ◽  
Béatrice Roy ◽  
...  
Keyword(s):  
Capillary Electrophoresis ◽  
Multiplexed Detection ◽  
Structure Switching
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