Direct analysis of in-gel proteins by carbon nanotubes-modified paper spray ambient mass spectrometry

The Analyst ◽  
2015 ◽  
Vol 140 (3) ◽  
pp. 710-715 ◽  
Author(s):  
Feifei Han ◽  
Yuhan Yang ◽  
Jin Ouyang ◽  
Na Na

The direct extraction, desorption and ionization of in gel-intact proteins after electrophoresis have been achieved by CNTs-modified paper spray MS at ambient conditions.

2021 ◽  
Vol 8 (1) ◽  
Author(s):  
Tyler J. Mason ◽  
Harmonie M. Bettenhausen ◽  
Jacqueline M. Chaparro ◽  
Mark E. Uchanski ◽  
Jessica E. Prenni

AbstractHorticulturists are interested in evaluating how cultivar, environment, or production system inputs can affect postharvest quality. Ambient mass spectrometry approaches enable analysis of minimally processed samples under ambient conditions and offer an attractive high-throughput alternative for assessing quality characteristics in plant products. Here, we evaluate direct analysis in real time (DART-MS) mass spectrometry and rapid evaporative ionization-mass spectrometry (REIMS) to assess quality characteristics in various pepper (Capsicum annuum L.) cultivars. DART-MS exhibited the ability to discriminate between pod colors and pungency based on chemical fingerprints, while REIMS could distinguish pepper market class (e.g., bell, lunchbox, and popper). Furthermore, DART-MS analysis resulted in the putative detection of important bioactive compounds in human diet such as vitamin C, p-coumaric acid, and capsaicin. The results of this study demonstrate the potential for these approaches as accessible and reliable tools for high throughput screening of pepper quality.


2017 ◽  
Vol 9 (34) ◽  
pp. 5037-5043 ◽  
Author(s):  
Rachel Jett ◽  
Christine Skaggs ◽  
Nicholas E. Manicke

Paper spray mass spectrometry is a direct analysis method in which compounds are extracted and ionized from biofluids dried on paper.


2015 ◽  
Vol 7 (23) ◽  
pp. 9808-9816 ◽  
Author(s):  
Steven L. Reeber ◽  
Sneha Gadi ◽  
Sung-Ben Huang ◽  
Gary L. Glish

Paper spray ionization enables the rapid mass spectrometric analysis of environmental samples without the use of chromatography or sample cleanup techniques.


2010 ◽  
Vol 49 (5) ◽  
pp. 877-880 ◽  
Author(s):  
He Wang ◽  
Jiangjiang Liu ◽  
R. Graham Cooks ◽  
Zheng Ouyang

2002 ◽  
Vol 74 (13) ◽  
pp. 2923-2929 ◽  
Author(s):  
Fanyu Meng ◽  
Benjamin J. Cargile ◽  
Steven M. Patrie ◽  
Jeffrey R. Johnson ◽  
Shaun M. McLoughlin ◽  
...  

2017 ◽  
Vol 9 (34) ◽  
pp. 4908-4923 ◽  
Author(s):  
Jin Chen ◽  
Fei Tang ◽  
Cheng'an Guo ◽  
Sichun Zhang ◽  
Xinrong Zhang

Plasma-based ambient mass spectrometry (AMS) exhibits great potential in the direct analysis of raw samples with minimum pretreatment.


2017 ◽  
Vol 9 (34) ◽  
pp. 5065-5074 ◽  
Author(s):  
Kathleen T. Upton ◽  
Katherine A. Schilling ◽  
J. L. Beauchamp

The EZ-DART ion source for ambient mass spectrometry, with simplified construction, minimal assembly cost, and versatile sampling capabilities, is described.


The Analyst ◽  
2013 ◽  
Vol 138 (16) ◽  
pp. 4624 ◽  
Author(s):  
Anyin Li ◽  
Pu Wei ◽  
Hsu-Chen Hsu ◽  
R. Graham Cooks

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