Sorting of human mesenchymal stem cells by applying optimally designed microfluidic chip filtration

The Analyst ◽  
2015 ◽  
Vol 140 (4) ◽  
pp. 1265-1274 ◽  
Author(s):  
Heekyung Jung ◽  
Myung-Suk Chun ◽  
Mi-Sook Chang
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Microfluidic Chip ◽  
Human Mesenchymal Stem Cells ◽  
Microfluidic Chips ◽  
Human Stem Cells ◽  
Hydrodynamic Filtration

This paper presents sorting of human stem cells by applying optimally designed microfluidic chips based on the hydrodynamic filtration principle.

scholarly journals Effect of TiO2 Nanotube Pore Diameter on Human Mesenchymal Stem Cells and Human Osteoblasts

Nanomaterials ◽  
2020 ◽  
Vol 10 (11) ◽  
pp. 2117
Author(s):  
Juan Shong Khaw ◽  
Christopher R. Bowen ◽  
Sarah H. Cartmell
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Pore Diameter ◽  
Human Mesenchymal Stem Cells ◽  
Tio2 Nanotube ◽  
Human Stem Cells ◽  
Material Interfaces ◽  
Human Osteoblasts ◽  
Cellular Behaviour ◽  
20 Nm

The pore diameter of uniformly structured nanotubes can significantly change the behaviour of cells. Recent studies demonstrated that the activation of integrins is affected not by only the surface chemistry between the cell-material interfaces, but also by the features of surface nanotopography, including nanotube diameter. While research has been carried out in this area, there has yet to be a single systemic study to date that succinctly compares the response of both human stem cells and osteoblasts to a range of TiO2 nanotube pore diameters using controlled experiments in a single laboratory. In this paper, we investigate the influence of surface nanotopography on cellular behaviour and osseointegrative properties through a systemic study involving human mesenchymal stem cells (hMSCs) and human osteoblasts (HOBs) on TiO2 nanotubes of 20 nm, 50 nm and 100 nm pore diameters using in-vitro assessments. This detailed study demonstrates the interrelationship between cellular behaviour and nanotopography, revealing that a 20 nm nanotube pore diameter is preferred by hMSCs for the induction of osteogenic differentiation, while 50 nm nanotubular structures are favourable by HOBs for osteoblastic maturation.

Dose-Dependent Immunomodulatory Effect of Human Stem Cells from Amniotic Membrane: A Comparison with Human Mesenchymal Stem Cells from Adipose Tissue

2007 ◽  
Vol 13 (6) ◽  
pp. 1173-1183 ◽  
Author(s):  
Susanne Wolbank ◽  
Anja Peterbauer ◽  
Marc Fahrner ◽  
Simone Hennerbichler ◽  
Martijn van Griensven ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Adipose Tissue ◽  
Amniotic Membrane ◽  
Human Mesenchymal Stem Cells ◽  
Immunomodulatory Effect ◽  
Human Stem Cells ◽  
Dose Dependent

scholarly journals Functionalized Silica Star-Shaped Nanoparticles and Human Mesenchymal Stem Cells: An In Vitro Model

Nanomaterials ◽  
2021 ◽  
Vol 11 (3) ◽  
pp. 779
Author(s):  
Chiara Argentati ◽  
Francesco Morena ◽  
Chiara Fontana ◽  
Ilaria Tortorella ◽  
Carla Emiliani ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Stem Cell ◽  
Silica Nanoparticles ◽  
Morphological Changes ◽  
Mesoporous Silica Nanoparticles ◽  
Human Mesenchymal Stem Cells ◽  
Growth Curves ◽  
Human Stem Cells

The biomedical translational applications of functionalized nanoparticles require comprehensive studies on their effect on human stem cells. Here, we have tested neat star-shaped mesoporous silica nanoparticles (s-MSN) and their chemically functionalized derivates; we examined nanoparticles (NPs) with similar dimensions but different surface chemistry, due to the amino groups grafted on silica nanoparticles (s-MSN-NH2), and gold nanoseeds chemically adsorbed on silica nanoparticles (s-MSN-Au). The different samples were dropped on glass coverslips to obtain a homogeneous deposition differing only for NPs’ chemical functionalization and suitable for long-term culture of human Bone Marrow–Mesenchymal stem cells (hBM-MSCs) and Adipose stem cells (hASCs). Our model allowed us to demonstrate that hBM-MSCs and hASCs have comparable growth curves, viability, and canonical Vinculin Focal adhesion spots on functionalized s-MSN-NH2 and s-MSN-Au as on neat s-MSN and control systems, but also to show morphological changes on all NP types compared to the control counterparts. The new shape was stem-cell-specific and was maintained on all types of NPs. Compared to the other NPs, s-MSN-Au exerted a small genotoxic effect on both stem cell types, which, however, did not affect the stem cell behavior, likely due to a peculiar stem cell metabolic restoration response.

Proliferation and multi-differentiation potentials of human mesenchymal stem cells on thermoresponsive PDMS surfaces grafted with PNIPAAm

2010 ◽  
Vol 30 (6) ◽  
pp. 455-455 ◽  
Author(s):  
Dongyan Shi ◽  
Dan Ma ◽  
Feiqing Dong ◽  
Chen Zong ◽  
Liyue Liu ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Human Mesenchymal Stem Cells

The Effect of Link N on the Differentiation of Human Mesenchymal Stem Cells

2012 ◽  
Vol 2 (1_suppl) ◽  
pp. s-0032-1320001-s-0032-1320001
Author(s):  
F. Mwale ◽  
H. T. Wang ◽  
L. Haglund ◽  
P. J. Roughley ◽  
J. Antoniou
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Human Mesenchymal Stem Cells

Generation of human mesenchymal stem cells lacking diabetoporosity-associated miRNAs using CRISPR/Cas9

2020 ◽  
Author(s):  
I Foessl ◽  
A Groselj-Strele ◽  
JC Piswanger-Sölkner ◽  
H Dobnig ◽  
A Fahrleitner-Pammer ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Human Mesenchymal Stem Cells
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