Label-free determination of lipid composition and secondary protein structure of human salivary noncancerous and cancerous tissues by Raman microspectroscopy

The Analyst ◽  
2015 ◽  
Vol 140 (7) ◽  
pp. 2107-2113 ◽  
Author(s):  
Beata Brozek-Pluska ◽  
Monika Kopec ◽  
Izabela Niedzwiecka ◽  
Alina Morawiec-Sztandera
Keyword(s):  
Protein Structure ◽  
Cancer Detection ◽  
Lipid Composition ◽  
Raman Microspectroscopy ◽  
Spectroscopic Methods ◽  
Label Free ◽  
Secondary Protein Structure ◽  
Oncological Diagnostics

The applications of optical spectroscopic methods in cancer detection open new possibilities in oncological diagnostics.

Label-Free Determination of the Cell Cycle Phase in Human Embryonic Stem Cells by Raman Microspectroscopy

2013 ◽  
Vol 85 (19) ◽  
pp. 8996-9002 ◽  
Author(s):  
Stanislav O. Konorov ◽  
H. Georg Schulze ◽  
James M. Piret ◽  
Michael W. Blades ◽  
Robin F. B. Turner
Keyword(s):  
Cell Cycle ◽  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Human Embryonic Stem Cells ◽  
Embryonic Stem ◽  
Raman Microspectroscopy ◽  
Cell Cycle Phase ◽  
Cycle Phase ◽  
Label Free

Raman Microspectroscopic Study of the Cation Distribution in Amphiboles

1988 ◽  
Vol 42 (8) ◽  
pp. 1441-1450 ◽  
Author(s):  
A. Wang ◽  
P. Dhamelincourt ◽  
G. Turrell
Keyword(s):  
Theoretical Analysis ◽  
Raman Spectra ◽  
Cation Distribution ◽  
Raman Microspectroscopy ◽  
Experimental Results ◽  
Spectroscopic Methods ◽  
Cation Distributions

The first attempt to determine the cation distributions in amphiboles by Raman microspectroscopy is described. The Raman spectra of five amphibole samples were obtained. It was observed that the Raman bands of the OH stretching modes were narrower than the corresponding infrared bands previously reported. However, even with the use of the most sophisticated Raman instruments, it was not possible to distinguish the bands due to the different cation arrangements of one particular configuration. Nevertheless, the experimental results and the theoretical analysis developed here allowed approximate calculations of the site occupancies to be made for four samples. These results yield a better understanding of the problems involved in the determination of the cation distributions in amphiboles with the use of vibrational spectroscopic methods.

scholarly journals Determination of spectral markers of cytotoxicity and genotoxicity using in vitro Raman microspectroscopy: cellular responses to polyamidoamine dendrimer exposure

The Analyst ◽  
2017 ◽  
Vol 142 (20) ◽  
pp. 3848-3856 ◽  
Author(s):  
Esen Efeoglu ◽  
Alan Casey ◽  
Hugh J. Byrne
Keyword(s):  
Cellular Responses ◽  
Raman Microspectroscopy ◽  
High Content Screening ◽  
Label Free ◽  
Polyamidoamine Dendrimer ◽  
Screening Technique

Raman microspectroscopy as anin vitrolabel-free, high content screening technique to determine spectral markers of cytogenotoxicity.

The protonation of the ethylenediphosphinetetraacetate anion and the crystal structure of the bis (hydrogen bromide) of ethylenediphosphinetetraacetic acid

1981 ◽  
Vol 46 (12) ◽  
pp. 3063-3073 ◽  
Author(s):  
Jana Podlahová ◽  
Bohumil Kratochvíl ◽  
Vratislav Langer ◽  
Josef Šilha ◽  
Jaroslav Podlaha
Keyword(s):  
Crystal Structure ◽  
Hydrogen Bonds ◽  
Structure Determination ◽  
Free Acid ◽  
Hydrogen Bromide ◽  
Carboxyl Groups ◽  
Spectroscopic Methods ◽  
X Ray ◽  
Partial Formation

The equilibria and mechanism of addition of protons to the ethylenediphosphinetetraacetate anion (L4-) were studied in solution by the UV, IR, 1H and 31P NMR spectroscopic methods. A total of six protons can be bonded to the anion. They are added stepwise, first with partial formation of zwitterions containing P-H bonds, which then dissociate with formation of the free acid, H4L, where all four protons are bonded in carboxyl groups. The formation of zwitterions is strongly dependent on the concentration. In the final stage, the acid bonds two additional protons to form the bis-phosphonium cation, H6L2+. A number of isostructural salts containing this cation, H4L.2 HX (X = Cl, Br, I), have been prepared. The X-ray crystal structure determination of the bromide confirmed the expected arrangement. The bromide crystals are monoclinic, a = 578.2, b = 1 425.0, c = 1 046.7 pm, β = 103.07° with a space group of P21/c, Z = 2. The final R factor was 0.059 based on 1 109 observed reflections. The structure consists of H6L2+ cations containing protons bonded to phosphorus atoms (P-H distance 134 pm) and of bromide anions, located in gaps which are also sufficiently large for I- anions in the isostructural iodide. The interbonding of phosphonium cations proceeds through hydrogen bonds, C-OH...O=C, in which the O...O distance is 275.3 pm.

scholarly journals A simple method for the determination of lipid composition of human bile.

1991 ◽  
Vol 32 (2) ◽  
pp. 371-374
Author(s):  
M Muraca ◽  
MT Vilei ◽  
L Miconi ◽  
P Petrin ◽  
M Antoniutti ◽  
...  
Keyword(s):  
Lipid Composition ◽  
Simple Method ◽  
Human Bile

Determination of Phosphate Phases in Sewage Sludge Ash-Based Fertilizers by Raman Microspectroscopy

2013 ◽  
Vol 67 (9) ◽  
pp. 1101-1105 ◽  
Author(s):  
Christian Vogel ◽  
Christian Adam ◽  
Don McNaughton
Keyword(s):  
Sewage Sludge ◽  
Raman Microspectroscopy ◽  
Sewage Sludge Ash ◽  
Sludge Ash
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