Mechanism of DNA trapping in nanoporous structures during asymmetric pulsed-field electrophoresis

The Analyst ◽  
2014 ◽  
Vol 139 (22) ◽  
pp. 6044-6051 ◽  
Author(s):  
Ya Zhou ◽  
Huiying Sheng ◽  
D. Jed Harrison
Keyword(s):  
Detailed Model ◽  
Pulsed Field ◽  
Pulsed Field Electrophoresis ◽  
High Fields ◽  
Dna Trapping ◽  
Nanoporous Structures

A detailed model of DNA trapping at high fields in particle-based sieves is developed, identifying di-electrophoresis as the primary cause.

Influence of the agarose matrix in pulsed-field electrophoresis

1993 ◽  
Vol 14 (1) ◽  
pp. 349-354 ◽  
Author(s):  
Francis H. Kirkpatrick ◽  
Maxine M. Dumais ◽  
Hugh W. White ◽  
Kenneth B. Guiseley
Keyword(s):  
Pulsed Field ◽  
Pulsed Field Electrophoresis

Genotyping of Ureaplasma diversum isolates using pulsed-field electrophoresis

2007 ◽  
Vol 173 (3) ◽  
pp. 688-690 ◽  
Author(s):  
Melissa Buzinhani ◽  
Marcos R. Buim ◽  
Maurício Yamaguti ◽  
Rosângela C. Oliveira ◽  
Elena Mettifogo ◽  
...  
Keyword(s):  
Pulsed Field ◽  
Pulsed Field Electrophoresis ◽  
Ureaplasma Diversum

DNA Dynamics in Nanoscale Confinement under Asymmetric Pulsed Field Electrophoresis

2010 ◽  
Vol 49 (19) ◽  
pp. 3326-3329 ◽  
Author(s):  
Neda Nazemifard ◽  
Subir Bhattacharjee ◽  
Jacob H. Masliyah ◽  
D. Jed Harrison
Keyword(s):  
Dna Dynamics ◽  
Pulsed Field ◽  
Pulsed Field Electrophoresis

scholarly journals Separation of Micronuclear DNA of Stylonychia lemnae by Pulsed-Field Electrophoresis and Identification of a DNA Molecule with a High Copy Number

1999 ◽  
Vol 9 (7) ◽  
pp. 654-661
Author(s):  
Christian Maercker ◽  
Heike Kortwig ◽  
Hans J. Lipps
Keyword(s):  
Copy Number ◽  
High Copy Number ◽  
Pulsed Field ◽  
Macronuclear Development ◽  
Dna Molecule ◽  
Pulsed Field Electrophoresis ◽  
Dna Fragment ◽  
Nuclear Differentiation ◽  
Excellent Tool ◽  
Very High

DNA from the hypotrichous ciliatae Stylonychia lemnae was separated by PFGE. In addition to the separation of the macronuclear DNA molecules with a size up to ∼40 kb, we were able to separate the micronuclear DNA with a size between ∼90 kb and 2 Mb. One very prominent 90-kb DNA band appeared on the pulsed-field gels. We propose that this 90-kb DNA fragment represents a linear plasmid residing in the micronucleus in a very high copy number. About 10% of the micronuclear DNA consists of the 90-kb DNA molecule. It appears in the micronucleus as well as in the macronuclear anlagen during macronuclear development but not in the mature macronucleus. Thus, the multicopy DNA is eliminated during fragmentation of the macronuclear anlagen DNA in the course of macronuclear development. Therefore, this 90-kb DNA molecule might serve as an excellent tool to study the recognition and elimination of DNA during nuclear differentiation of hypotrichous ciliates.

scholarly journals "Analysis of the Anaplasma mavginale genome by pulsed-field electrophoresis"

1993 ◽  
Vol 139 (10) ◽  
pp. 2439-2444 ◽  
Author(s):  
R. Alleman ◽  
M. S. Kamper ◽  
N. Viseshakul ◽  
A. F. Barbet
Keyword(s):  
Pulsed Field ◽  
Pulsed Field Electrophoresis
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