scholarly journals Development of an ELISA microarray assay for the sensitive and simultaneous detection of ten biodefense toxins

The Analyst ◽  
2014 ◽  
Vol 139 (20) ◽  
pp. 5093-5102 ◽  
Author(s):  
Kathryn L. Jenko ◽  
Yanfeng Zhang ◽  
Yulia Kostenko ◽  
Yongfeng Fan ◽  
Consuelo Garcia-Rodriguez ◽  
...  
Keyword(s):  
Protein Microarray ◽  
Simultaneous Detection ◽  
Microarray Assay

An ELISA-based protein microarray was developed for the sensitive and simultaneous detection of 10 biodefense toxins.

scholarly journals Spectral-domain optical coherence phase microscopy for label-free multiplexed protein microarray assay

2009 ◽  
Vol 25 (2) ◽  
pp. 275-281 ◽  
Author(s):  
Chulmin Joo ◽  
Emre Özkumur ◽  
M. Selim Ünlü ◽  
Johannes F. de Boer
Keyword(s):  
Protein Microarray ◽  
Spectral Domain ◽  
Optical Coherence ◽  
Label Free ◽  
Microarray Assay ◽  
Phase Microscopy

An antibody-based microarray assay for the simultaneous detection of aflatoxin B1 and fumonisin B1

2009 ◽  
Vol 25 (4) ◽  
pp. 193-200 ◽  
Author(s):  
Ilaria Lamberti ◽  
Caterina Tanzarella ◽  
Isabella Solinas ◽  
Cristiano Padula ◽  
Lucia Mosiello
Keyword(s):  
Aflatoxin B1 ◽  
Simultaneous Detection ◽  
Fumonisin B1 ◽  
Microarray Assay

scholarly journals Development of a DNA microarray assay for rapid detection of fifteen bacterial pathogens in pneumonia

2020 ◽  
Author(s):  
Xiuqing Ma ◽  
Yanqin Li ◽  
Yuan Liang ◽  
Yang Liu ◽  
Ling Yu ◽  
...  
Keyword(s):  
Dna Microarray ◽  
Legionella Pneumophila ◽  
Burkholderia Cepacia ◽  
Pathogenic Bacteria ◽  
Chlamydia Pneumoniae ◽  
Bacterial Pathogens ◽  
Bacterial Species ◽  
Simultaneous Detection ◽  
Clinical Isolates ◽  
Microarray Assay

Abstract Background: The rapid identification of pathogenic bacteria is important for determining an appropriate antimicrobial therapy for pneumonia, but traditional bacterial culture is time-consuming and labourious. The aim of this study was to develop and evaluate a DNA microarray assay for the simultaneous detection of fifteen bacterial species directly from respiratory tract specimens in patients with pneumonia. These species included Streptococcus pneumoniae, Staphylococcus aureus, Haemophilus influenzae, Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa, Acinetobacter baumannii, Mycoplasma pneumoniae, Enterococcus faecalis, Enterococcus faecium, Enterobacter cloacae, Stenotrophomonas maltophilia, Burkholderia cepacia, Legionella pneumophila and Chlamydia pneumoniae. The 16S rDNA genes and other specific genes of each pathogen were chosen as the amplification targets, amplified via multiplex polymerase chain reaction (PCR), and hybridized to oligonucleotide probes in a microarray.Results: The DNA microarray detection limit was 103 copies/μL. Nineteen standard strains and 119 clinical isolates were correctly detected with our microarray, and 3 nontarget species from 4 clinical isolates were not detected. Additionally, bacterial pathogens were accurately identified when two or three bacterial targets were mixed together. Furthermore, the results for 99.4% (156/157) of clinical specimens were the same as those from a conventional assay.Conclusions: We developed a DNA microarray that could simultaneously detect various bacterial pathogens in pneumonia. The method described here has the potential to provide considerable labour and time savings due to its ability to screen for 15 bacterial pathogens simultaneously.

Sign in / Sign up

Export Citation Format

Share Document