scholarly journals The multivariate detection limit for Mycoplasma pneumoniae as determined by nanorod array-surface enhanced Raman spectroscopy and comparison with limit of detection by qPCR

The Analyst ◽  
2014 ◽  
Vol 139 (24) ◽  
pp. 6426-6434 ◽  
Author(s):  
Kelley C. Henderson ◽  
Edward S. Sheppard ◽  
Omar E. Rivera-Betancourt ◽  
Joo-Young Choi ◽  
Richard A. Dluhy ◽  
...  
Keyword(s):  
Raman Spectroscopy ◽  
Detection Limit ◽  
Mycoplasma Pneumoniae ◽  
Limit Of Detection ◽  
Bacterial Pathogen ◽  
Detection Limits ◽  
Nanorod Array ◽  
Surface Enhanced Raman Spectroscopy ◽  
Surface Enhanced ◽  
Surface Enhanced Raman

The detection limits by NA-SERS and qPCR for the bacterial pathogenMycoplasma pneumoniaewere compared.

scholarly journals Detection and Differentiation of Avian Mycoplasmas by Surface-Enhanced Raman Spectroscopy Based on a Silver Nanorod Array

2011 ◽  
Vol 78 (6) ◽  
pp. 1930-1935 ◽  
Author(s):  
Suzanne L. Hennigan ◽  
Jeremy D. Driskell ◽  
Naola Ferguson-Noel ◽  
Richard A. Dluhy ◽  
Yiping Zhao ◽  
...  
Keyword(s):  
Raman Spectroscopy ◽  
Sensitivity And Specificity ◽  
Limit Of Detection ◽  
Direct Detection ◽  
Nanorod Array ◽  
Surface Enhanced Raman Spectroscopy ◽  
Mycoplasma Species ◽  
Content Type ◽  
Surface Enhanced ◽  
Surface Enhanced Raman

ABSTRACTMycoplasma gallisepticumis a bacterial pathogen of poultry that is estimated to cause annual losses exceeding $780 million. The National Poultry Improvement Plan guidelines recommend regular surveillance and intervention strategies to containM. gallisepticuminfections and ensure mycoplasma-free avian stocks, but several factors make detection ofM. gallisepticumand diagnosis ofM. gallisepticuminfection a major challenge. Current techniques are laborious, require special expertise, and are typically plagued by false results. In this study, we describe a novel detection strategy which uses silver nanorod array–surface-enhanced Raman spectroscopy (NA-SERS) for direct detection of avian mycoplasmas. As a proof of concept for use in avian diagnostics, we used NA-SERS to detect and differentiate multiple strains of avian mycoplasma species, includingAcholeplasma laidlawii,Mycoplasma gallinarum,Mycoplasma gallinaceum,Mycoplasma synoviae, andM. gallisepticum, including vaccine strains 6/85, F, and ts-11. Chemometric multivariate analysis of spectral data was used to classify these species rapidly and accurately, with >93% sensitivity and specificity. Furthermore, NA-SERS had a lower limit of detection that was 100-fold greater than that of standard PCR and comparable to that of real-time quantitative PCR. Detection ofM. gallisepticumin choanal cleft swabs from experimentally infected birds yielded good sensitivity and specificity, suggesting that NA-SERS is applicable for clinical detection.

scholarly journals Detection of Mycoplasma pneumoniae in Simulated and True Clinical Throat Swab Specimens by Nanorod Array-Surface-Enhanced Raman Spectroscopy

PLoS ONE ◽  
2010 ◽  
Vol 5 (10) ◽  
pp. e13633 ◽  
Author(s):  
Suzanne L. Hennigan ◽  
Jeremy D. Driskell ◽  
Richard A. Dluhy ◽  
Yiping Zhao ◽  
Ralph A. Tripp ◽  
...  
Keyword(s):  
Raman Spectroscopy ◽  
Mycoplasma Pneumoniae ◽  
Throat Swab ◽  
Nanorod Array ◽  
Surface Enhanced Raman Spectroscopy ◽  
Surface Enhanced ◽  
Surface Enhanced Raman

scholarly journals Specificity and Strain-Typing Capabilities of Nanorod Array-Surface Enhanced Raman Spectroscopy for Mycoplasma pneumoniae Detection

PLoS ONE ◽  
2015 ◽  
Vol 10 (6) ◽  
pp. e0131831 ◽  
Author(s):  
Kelley C. Henderson ◽  
Alvaro J. Benitez ◽  
Amy E. Ratliff ◽  
Donna M. Crabb ◽  
Edward S. Sheppard ◽  
...  
Keyword(s):  
Raman Spectroscopy ◽  
Mycoplasma Pneumoniae ◽  
Nanorod Array ◽  
Surface Enhanced Raman Spectroscopy ◽  
Strain Typing ◽  
Surface Enhanced ◽  
Surface Enhanced Raman

scholarly journals Detection of Triphenylmethane Drugs in Fish Muscle by Surface-Enhanced Raman Spectroscopy Coupled with Au-Ag Core-Shell Nanoparticles

2014 ◽  
Vol 2014 ◽  
pp. 1-8 ◽  
Author(s):  
Lu Pei ◽  
Yiqun Huang ◽  
Chunying Li ◽  
Yuanyuan Zhang ◽  
Barbara A. Rasco ◽  
...  
Keyword(s):  
Raman Spectroscopy ◽  
Malachite Green ◽  
Crystal Violet ◽  
Bimetallic Nanoparticles ◽  
Limit Of Detection ◽  
Fish Muscle ◽  
Surface Enhanced Raman Spectroscopy ◽  
Vis Spectroscopy ◽  
Surface Enhanced ◽  
Surface Enhanced Raman

Silver-coated gold bimetallic nanoparticles were synthesized and used as substrates for surface-enhanced Raman spectroscopy (SERS) in detecting prohibited triphenylmethane drugs (including crystal violet and malachite green) in fish muscle. The optical properties and physical properties of bimetallic nanospheres were characterized by UV-Vis spectroscopy and transmission electron microscopy. The optimal nanospheres selected had relatively uniform size (diameter: 33 ± 3 nm) with a silver layer coated on the surface of gold seed (diameter: 18 ± 2 nm). For both crystal violet and malachite green, characteristic SERS spectral features could be identified at concentration as low as 0.1 μg/L with these bimetallic nanospheres. Crystal violet and malachite green residues in fish muscle could also be detected at levels as low as 0.1 ng/g, which could meet the most restricted regulatory requirements for the limit of detection in terms of analytical methods for crystal violet or malachite green in fish muscle. This study provides a basis for applying SERS technology with bimetallic nanoparticles to the identification of trace amounts of prohibited substances in aquatic food products, and the methodology could be extended to analyses of other hazardous chemicals in complex food matrices like vegetables and meats.

Methodologies for assessment of limit of detection and limit of identification using surface-enhanced Raman spectroscopy

2015 ◽  
Vol 207 ◽  
pp. 437-446 ◽  
Author(s):  
Enrico Massarini ◽  
Pär Wästerby ◽  
Lars Landström ◽  
Christian Lejon ◽  
Olof Beck ◽  
...  
Keyword(s):  
Raman Spectroscopy ◽  
Limit Of Detection ◽  
Surface Enhanced Raman Spectroscopy ◽  
Surface Enhanced ◽  
Surface Enhanced Raman

scholarly journals Cascaded microsphere-coupled surface-enhanced Raman spectroscopy (CMS-SERS) for ultrasensitive trace-detection

Nanophotonics ◽  
2022 ◽  
Vol 0 (0) ◽  
Author(s):  
Yanlin Mi ◽  
Yinzhou Yan ◽  
Mengyuan Wang ◽  
Lixue Yang ◽  
Jing He ◽  
...  
Keyword(s):  
Raman Spectroscopy ◽  
Limit Of Detection ◽  
Surface Enhanced Raman Spectroscopy ◽  
Sers Substrate ◽  
Trace Detection ◽  
Excitation Light ◽  
Analytical Technique ◽  
Nano Structures ◽  
Surface Enhanced ◽  
Surface Enhanced Raman

Abstract Surface-enhanced Raman spectroscopy (SERS) has been widely investigated and employed as a powerful optical analytical technique providing fingerprint vibrational information of molecules with high sensitivity and resolution. In addition to metallic nanostructure, dielectric micro-/nano-structures with extraordinary optical manipulation properties have demonstrated capability in enhanced Raman scattering with ultralow energy losses. Here we report a facile cascaded structure composed of a large microsphere (LMS) and a small microsphere array with Ag nanoparticles as a novel hybrid SERS substrate, for the first time. The cascaded microsphere-coupled SERS substrate provides a platform to increase the molecular concentration, boost the intensity of localized excitation light, and direct the far-field emission, for giant Raman enhancement. It demonstrates the maximum enhancement factor of Raman intensity greater than 108 for the limit of detection down to 10−11 M of 4-nitrothiphenol molecules in aqueous solution. The present work inspires a novel strategy to fabricate cascaded dielectric/metallic micro-/nano-structures superior to traditional SERS substrates towards practical applications in cost-effective and ultrahigh-sensitive trace-detection.

scholarly journals Liquid Surface-Enhanced Raman Spectroscopy (SERS) Sensor-Based Au-Ag Colloidal Nanoparticles for Easy and Rapid Detection of Deltamethrin Pesticide in Brewed Tea

Crystals ◽  
2021 ◽  
Vol 12 (1) ◽  
pp. 24
Author(s):  
Affi Nur Hidayah ◽  
Djoko Triyono ◽  
Yuliati Herbani ◽  
Rosari Saleh
Keyword(s):  
Raman Spectroscopy ◽  
Liquid Surface ◽  
Limit Of Detection ◽  
Surface Enhanced Raman Spectroscopy ◽  
Ease Of Use ◽  
Raman Signal ◽  
Colloidal Nanoparticles ◽  
Sers Substrate ◽  
Surface Enhanced ◽  
Surface Enhanced Raman

Deltamethrin pesticides can cause inflammation, nephrotoxicity and hepatotoxicity as well as affect the activity of antioxidant enzymes in tissues. As a result of this concern, there is a rising focus on the development of fast and reliable pesticide residue testing to minimise potential risks to humans. The goal of this study is to use Au-Ag colloid nanoparticles as liquid surface-enhanced Raman spectroscopy (SERS) to improve the Raman signal in the detection of deltamethrin pesticide in a brewed tea. The liquid SERS system is fascinating to study due to its ease of use and its unlikeliness to cause several phenomena, such as photo-bleaching, combustion, sublimation and even photo-catalysis, which can interfere with the Raman signal, as shown in the SERS substrate. Our liquid SERS system is simpler than previous liquid SERS systems that have been reported. We performed the detection of pesticide analyte directly on brewed tea, without diluting it with ethanol or centrifuging it. Femtosecond laser-induced photo-reduction was employed to synthesise the liquid SERS of Au, Au-Ag, and Ag colloidal nanoparticles. The SERS was utilised to detect deltamethrin pesticide in brewed tea. The result showed that liquid SERS-based Ag NPs significantly enhance the Raman signal of pesticides compared with liquid SERS-based Au NPs and Au-Ag Nanoalloys. The maximum residue limits (MRLs) in tea in Indonesia are set at 10 ppm. Therefore, this method was also utilised to detect and improve, to 0.01 ppm, the deltamethrin pesticide Limit of Detection (LOD).

scholarly journals Surface Enhanced Raman Spectroscopy for DNA Biosensors—How Far Are We?

Molecules ◽  
2019 ◽  
Vol 24 (24) ◽  
pp. 4423 ◽  
Author(s):  
Edyta Pyrak ◽  
Jan Krajczewski ◽  
Artur Kowalik ◽  
Andrzej Kudelski ◽  
Aleksandra Jaworska
Keyword(s):  
Raman Spectroscopy ◽  
Limit Of Detection ◽  
Surface Enhanced Raman Spectroscopy ◽  
Diagnostic Tools ◽  
Strong Increase ◽  
Raman Signal ◽  
Silver Nanostructures ◽  
Dna Sensors ◽  
Surface Enhanced ◽  
Surface Enhanced Raman

A sensitive and accurate identification of specific DNA fragments (usually containing a mutation) can influence clinical decisions. Standard methods routinely used for this type of detection are PCR (Polymerase Chain Reaction, and its modifications), and, less commonly, NGS (Next Generation Sequencing). However, these methods are quite complicated, requiring time-consuming, multi-stage sample preparation, and specially trained staff. Usually, it takes weeks for patients to obtain their results. Therefore, different DNA sensors are being intensively developed by many groups. One technique often used to obtain an analytical signal from DNA sensors is Raman spectroscopy. Its modification, surface-enhanced Raman spectroscopy (SERS), is especially useful for practical analytical applications due to its extra low limit of detection. SERS takes advantage of the strong increase in the efficiency of Raman signal generation caused by a local electric field enhancement near plasmonic (typically gold and silver) nanostructures. In this condensed review, we describe the most important types of SERS-based nanosensors for genetic studies and comment on their potential for becoming diagnostic tools.

scholarly journals Comparative Performance of Citrate, Borohydride, Hydroxylamine and β-Cyclodextrin Silver Sols for Detecting Ibuprofen and Caffeine Pollutants by Means of Surface-Enhanced Raman Spectroscopy

Nanomaterials ◽  
2020 ◽  
Vol 10 (12) ◽  
pp. 2339
Author(s):  
Michele Lemos de Souza ◽  
Juan Carlos Otero ◽  
Isabel López-Tocón
Keyword(s):  
Raman Spectroscopy ◽  
Emerging Contaminants ◽  
Limit Of Detection ◽  
Surface Enhanced Raman Spectroscopy ◽  
Molar Ratio ◽  
Sers Substrate ◽  
Comparative Performance ◽  
Sers Signal ◽  
Surface Enhanced ◽  
Surface Enhanced Raman

The detection of emerging contaminants in the aquatic environment, such as ibuprofen and caffeine, was studied by means of surface-enhanced Raman spectroscopy (SERS) using Ag nanoparticles (AgNPs) synthesized with β-cyclodextrin (βCD) as a reducing agent. The effect on the SERS signal of different molar ratios of Ag+/βCD in the synthesis route and the aging process of AgNPs were investigated by using trans-cinnamic as a test molecule. The SERS effectiveness of these β-cyclodextrin colloids (Ag@βCD) was also checked and compared with that of other silver sols usually employed in SERS synthesized by using other reducing agents such as citrate, borohydride and hydroxylamine. All the synthesized SERS substrates were characterized by different techniques. The experimental results indicate that Ag@βCD with the more diluted Ag+/βCD molar ratio showed the best SERS signal, enabling detection at trace concentrations of 0.5 µM in the case of trans-cinnamic acid. The Ag@βCD sols also showed the best sensitivity for detecting ibuprofen and caffeine, reaching the lowest limit of detection (0.1 mM). The proposed synthetic route for Ag@βCD sols provides an improved SERS substrate for detecting organic pollutants with better performance than other standard silver sols.

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