In Situ metabolic analysis of single plant cells by capillary microsampling and electrospray ionization mass spectrometry with ion mobility separation

The Analyst ◽  
2014 ◽  
Vol 139 (20) ◽  
pp. 5079-5085 ◽  
Author(s):  
Linwen Zhang ◽  
Daniel P. Foreman ◽  
Paaqua A. Grant ◽  
Bindesh Shrestha ◽  
Sally A. Moody ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Electrospray Ionization ◽  
Ion Mobility ◽  
Plant Cells ◽  
Ionization Mass ◽  
Single Plant ◽  
Mobility Separation ◽  
Metabolic Analysis ◽  
Ion Mobility Separation

In situ isobaric lipid mapping by MALDI-ion mobility separation-mass spectrometry imaging

2020 ◽  
Vol 55 (9) ◽  
pp. e4531 ◽  
Author(s):  
Tingting Fu ◽  
Janina Oetjen ◽  
Manuel Chapelle ◽  
Alexandre Verdu ◽  
Matthias Szesny ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Ion Mobility ◽  
Mass Spectrometry Imaging ◽  
Mobility Separation ◽  
Ion Mobility Separation

Spatial Metabolomics of the Human Kidney Using MALDI Trapped Ion Mobility Imaging Mass Spectrometry

2020 ◽  
Author(s):  
Elizabeth Neumann ◽  
Lukasz Migas ◽  
Jamie L. Allen ◽  
Richard Caprioli ◽  
Raf Van de Plas ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Ion Mobility ◽  
Imaging Mass Spectrometry ◽  
Structural Complexity ◽  
Human Kidney ◽  
Resolving Power ◽  
Mobility Separation ◽  
Trapped Ion ◽  
Mobility Data ◽  
Ion Mobility Separation

<div> <div> <p>Small metabolites are essential for normal and diseased biological function but are difficult to study because of their inherent structural complexity. MALDI imaging mass spectrometry (IMS) of small metabolites is particularly challenging as MALDI matrix clusters are often isobaric with metabolite ions, requiring high resolving power instrumentation or derivatization to circumvent this issue. An alternative to this is to perform ion mobility separation before ion detection, enabling the visualization of metabolites without the interference of matrix ions. Here, we use MALDI timsTOF IMS to image small metabolites at high spatial resolution within the human kidney. Through this, we have found metabolites, such as arginic acid, acetylcarnitine, and choline that localize to the cortex, medulla, and renal pelvis, respectively. We have also demonstrated that trapped ion mobility spectrometry (TIMS) can resolve matrix peaks from metabolite signal and separate both isobaric and isomeric metabolites with different localizations within the kidney. The added ion mobility data dimension dramatically increased the peak capacity for molecular imaging experiments. Future work will involve further exploring the small metabolite profiles of human kidneys as a function of age, gender, and ethnicity.</p></div></div>

Time-resolved in situ monitoring of photocatalytic reactions by probe electrospray ionization mass spectrometry

The Analyst ◽  
2020 ◽  
Vol 145 (9) ◽  
pp. 3313-3319
Author(s):  
Zhongbao Han ◽  
Xiaoyu Gu ◽  
Shirong Wang ◽  
Liyan Liu ◽  
Ying Wang ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Electrospray Ionization ◽  
Real Time ◽  
Electrospray Ionization Mass Spectrometry ◽  
In Situ Monitoring ◽  
Ionization Mass ◽  
Time Resolved ◽  
Photocatalytic Reactions ◽  
Probe Electrospray Ionization

We report the application of PESI-MS to in situ monitoring of photocatalytic reactions of cationic dyes in suspensions in real-time.

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