Webcam-based flow cytometer using wide-field imaging for low cell number detection at high throughput

The Analyst ◽  
2014 ◽  
Vol 139 (17) ◽  
pp. 4322-4329 ◽  
Author(s):  
Joshua Balsam ◽  
Hugh Alan Bruck ◽  
Avraham Rasooly
Keyword(s):  
Flow Cytometry ◽  
High Throughput ◽  
Large Volume ◽  
Current Flow ◽  
Low Cost ◽  
Cell Number ◽  
Flow Cytometer ◽  
Wide Field ◽  
Field Imaging ◽  
Wide Field Imaging

Here we describe a novel low-cost high throughput flow cytometer based on a webcam capable of low cell number detection in a large volume which may overcome the limitations of current flow cytometry.

scholarly journals Low cost multi-purpose balloon-borne platform for wide-field imaging and video observation

2016 ◽  
Author(s):  
Francisco Ocaña ◽  
Alejandro Sánchez de Miguel ◽  
Aitor Conde
Keyword(s):  
Low Cost ◽  
Video Observation ◽  
Wide Field ◽  
Field Imaging ◽  
Wide Field Imaging

scholarly journals Отложенная регистрация фотолюминесценции нанофосфоров как платформа для оптического биоимиджинга-=SUP=-*-=/SUP=-

2019 ◽  
Vol 126 (1) ◽  
pp. 90
Author(s):  
Е.В. Хайдуков ◽  
К.Н. Болдырев ◽  
К.В. Хайдуков ◽  
И.В. Крылов ◽  
И.М. Ашарчук ◽  
...  
Keyword(s):  
Time Delay ◽  
Low Cost ◽  
Imaging Systems ◽  
Resonance Excitation ◽  
Wide Field ◽  
Luminescence Signal ◽  
Biological Objects ◽  
Anti Stokes ◽  
Field Imaging ◽  
Wide Field Imaging

AbstractDetection systems with deferred registration of luminescence signals are promising for performing complex tasks of imaging of biological objects due to their simplicity and low cost. In the present work, β‑NaYF_4:Tm^3+Yb^3+/NaYF_4 nanocrystals with anti-Stokes photoluminescence have been used in deferred registration systems. It has been shown that there is a significant time delay between the exciting laser pulse and luminescence signal, which makes it possible to use this class of nanoparticles in the creation of wide-field imaging systems with deferred registration. The possibility of using nanoparticles for detecting a photoluminescence signal in the second transparency window of biotissue has been demonstrated. This system can be based on the resonance excitation and detection of the photoluminescence signal of Yb^3 + ions.

scholarly journals Camera-based localization microscopy optimized with calibrated structured illumination

2021 ◽  
Vol 4 (1) ◽  
Author(s):  
Martin Schmidt ◽  
Adam C. Hundahl ◽  
Henrik Flyvbjerg ◽  
Rodolphe Marie ◽  
Kim I. Mortensen
Keyword(s):  
Data Analysis ◽  
Super Resolution ◽  
Wide Field ◽  
Structured Illumination ◽  
Uniform Illumination ◽  
Localization Microscopy ◽  
Localization And Tracking ◽  
Field Imaging ◽  
Wide Field Imaging ◽  
Localization Information

AbstractUntil very recently, super-resolution localization and tracking of fluorescent particles used camera-based wide-field imaging with uniform illumination. Then it was demonstrated that structured illuminations encode additional localization information in images. The first demonstration of this uses scanning and hence suffers from limited throughput. This limitation was mitigated by fusing camera-based localization with wide-field structured illumination. Current implementations, however, use effectively only half the localization information that they encode in images. Here we demonstrate how all of this information may be exploited by careful calibration of the structured illumination. Our approach achieves maximal resolution for given structured illumination, has a simple data analysis, and applies to any structured illumination in principle. We demonstrate this with an only slightly modified wide-field microscope. Our protocol should boost the emerging field of high-precision localization with structured illumination.

scholarly journals Protocol for constructing an extensive cranial window utilizing a PEO-CYTOP nanosheet for in vivo wide-field imaging of the mouse brain

2021 ◽  
Vol 2 (2) ◽  
pp. 100542
Author(s):  
Taiga Takahashi ◽  
Hong Zhang ◽  
Kohei Otomo ◽  
Yosuke Okamura ◽  
Tomomi Nemoto
Keyword(s):  
Mouse Brain ◽  
Wide Field ◽  
Cranial Window ◽  
Field Imaging ◽  
Wide Field Imaging

scholarly journals The Virmos Project

1998 ◽  
Vol 11 (1) ◽  
pp. 492-492
Author(s):  
D. MacCagni ◽  
O. Le Fèvre ◽  
G. Vettolani ◽  
D. Mancini ◽  
J.P. Picat ◽  
...  
Keyword(s):  
Large Scale ◽  
Large Scale Structure ◽  
Scale Structure ◽  
Structure Evolution ◽  
Wide Field ◽  
Look Back ◽  
Field Imaging ◽  
Wide Field Imaging

Large and deep spectroscopic samples of galaxies are essential to study galaxies and large scale structure evolution out to look-back times ~ 10% the current age of the vmiverse. Keeping this scientific and observational goal in mind, we designed and are presently building two wide-field imaging spectrographs to be installed at the Nasmyth foci of the ESO-VLT Unit Telescopes 3 and 4.

scholarly journals Label Free High Speed Wide Field Imaging of Single Microtubules using Interference Reflection Microscopy

2018 ◽  
Vol 114 (3) ◽  
pp. 504a-505a
Author(s):  
Mohammed Mahamdeh ◽  
Steve Simmert ◽  
Anna Łuchniak ◽  
Erik Schäffer ◽  
Jonathon Howard
Keyword(s):  
High Speed ◽  
Wide Field ◽  
Label Free ◽  
Field Imaging ◽  
Wide Field Imaging

The Near-Earth Asteroid Tracking (NEAT) Program: An Automated System for Telescope Control, Wide-Field Imaging, and Object Detection

1999 ◽  
Vol 117 (3) ◽  
pp. 1616-1633 ◽  
Author(s):  
Steven H. Pravdo ◽  
David L. Rabinowitz ◽  
Eleanor F. Helin ◽  
Kenneth J. Lawrence ◽  
Raymond J. Bambery ◽  
...  
Keyword(s):  
Object Detection ◽  
Automated System ◽  
Wide Field ◽  
Earth Asteroid ◽  
Field Imaging ◽  
Wide Field Imaging
Sign in / Sign up

Export Citation Format

Share Document