Versatile chiral chromatography with mixed stationary phases of water-impregnated silica gel and reversed-phase packing

The Analyst ◽  
2014 ◽  
Vol 139 (8) ◽  
pp. 1830 ◽  
Author(s):  
Satsuki Takahashi ◽  
Tetsuo Okada
Keyword(s):  
Silica Gel ◽  
Stationary Phases ◽  
Reversed Phase ◽  
Chiral Chromatography ◽  
Mixed Stationary Phases ◽  
Reversed Phase Packing

Chiral Chromatography Studies of Chemical Behavior of Cinacalcet on Polysaccharide Chiral Reversed-Phase HPLC Stationary Phases

2012 ◽  
Vol 95 (6) ◽  
pp. 1639-1643 ◽  
Author(s):  
Michal Douša ◽  
Jiřiacute; Břicháč
Keyword(s):  
Chiral Stationary Phase ◽  
Stationary Phases ◽  
Active Pharmaceutical Ingredient ◽  
Reversed Phase ◽  
Hplc Method ◽  
Reversed Phase Hplc ◽  
Chiral Chromatography ◽  
Chemical Behavior ◽  
Analytical Resolution

Abstract A rapid HPLC method for the analytical resolution of cinacalcet enantiomers was developed. Four chiral columns (two amylose and two cellulose type) were evaluated in RP systems. Excellent enantioseparation with a resolution of more than 6 was achieved on Chiralpak AY (amylose 5-chloro-2-methylphenylcarbamate chiral stationary phase) using 10 mM triethylamine (pH 8.0)–acetonitrile (40 + 60, v/v) mobile phase. Validation of the HPLC method, including linearity, LOD, LOQ, precision, accuracy, and selectivity, was performed according to the International Conference on Harmonization guidelines. The method was successfully applied for the determination of (S)-cinacalcet in enantiopure active pharmaceutical ingredient (R)-cinacalcet.

Retention of some heterocyclic amines on mixed stationary phases containing nickel(II) Schiff base chelates

1989 ◽  
Vol 54 (6) ◽  
pp. 1530-1537 ◽  
Author(s):  
Joanna Masłowska ◽  
Grzegorz Bazylak
Keyword(s):  
Schiff Base ◽  
Stationary Phases ◽  
Capillary Columns ◽  
Heterocyclic Amines ◽  
Complex Mixtures ◽  
Amine Complexes ◽  
Mixed Stationary Phases ◽  
Classical Columns

Stationary phases composed of squalane and some nickel(II)-β-keto amine complexes were prepared and used for the separation of complex mixtures of pyridines. The resolution achieved on short classical columns was comparable with that obtained on capillary columns.

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