scholarly journals Nano-structured gelatin/bioactive glass hybrid scaffolds for the enhancement of odontogenic differentiation of human dental pulp stem cells

2013 ◽  
Vol 1 (37) ◽  
pp. 4764 ◽  
Author(s):  
Tiejun Qu ◽  
Xiaohua Liu
Keyword(s):  
Stem Cells ◽  
Bioactive Glass ◽  
Dental Pulp ◽  
Dental Pulp Stem Cells ◽  
Odontogenic Differentiation ◽  
Human Dental Pulp ◽  
Hybrid Scaffolds

scholarly journals Nanoparticles of Bioactive Glass Enhance Biodentine Bioactivity on Dental Pulp Stem Cells

Materials ◽  
2021 ◽  
Vol 14 (10) ◽  
pp. 2684
Author(s):  
Camila Corral Nunez ◽  
Diego Altamirano Gaete ◽  
Miguel Maureira ◽  
Javier Martin ◽  
Cristian Covarrubias
Keyword(s):  
Stem Cells ◽  
Cell Viability ◽  
Bioactive Glass ◽  
Dental Pulp ◽  
Sol Gel ◽  
Glass Ionomer Cement ◽  
Dental Pulp Stem Cells ◽  
Alp Activity ◽  
Odontogenic Differentiation ◽  
Human Dental Pulp

This study aimed to investigate the cytotoxicity and bioactivity of a novel nanocomposite containing nanoparticles of bioactive glass (nBGs) on human dental pulp stem cells (hDPSCs). nBGs were synthesized by the sol–gel method. Biodentine (BD) nanocomposites (nBG/BD) were prepared with 2 and 5% wt of nBG content; unmodified BD and glass ionomer cement were used as references. Cell viability and attachment were evaluated after 3, 7 and 14 days. Odontogenic differentiation was assessed with alkaline phosphatase (ALP) activity after 7 and 14 days of exposure. Cells successfully adhered and proliferated on nBG/BD nanocomposites, cell viability of nanocomposites was comparable with unmodified BD and higher than GIC. nBG/BD nanocomposites were, particularly, more active to promote odontogenic differentiation, expressed as higher ALP activity of hDPSCs after 7 days of exposure, than neat BD or GIC. This novel nanocomposite biomaterial, nBG/BD, allowed hDPSC attachment and proliferation and increased the expression of ALP, upregulated in mineral-producing cells. These findings open opportunities to use nBG/BD in vital pulp therapies.

scholarly journals The role of osteomodulin on osteo/odontogenic differentiation in human dental pulp stem cells

2019 ◽  
Vol 19 (1) ◽  
Author(s):  
Wenzhen Lin ◽  
Li Gao ◽  
Wenxin Jiang ◽  
Chenguang Niu ◽  
Keyong Yuan ◽  
...  
Keyword(s):  
Stem Cells ◽  
Dental Pulp ◽  
Dental Pulp Stem Cells ◽  
Odontogenic Differentiation ◽  
Human Dental Pulp

scholarly journals Pentraxin-3 Modulates Osteogenic/Odontogenic Differentiation and Migration of Human Dental Pulp Stem Cells

2019 ◽  
Vol 20 (22) ◽  
pp. 5778
Author(s):  
Yeon Kim ◽  
Joo-Yeon Park ◽  
Hyun-Joo Park ◽  
Mi-Kyoung Kim ◽  
Yong-Il Kim ◽  
...  
Keyword(s):  
Stem Cells ◽  
Dental Pulp ◽  
Tissue Repair ◽  
Therapeutic Potential ◽  
Dental Pulp Stem Cells ◽  
Pentraxin 3 ◽  
Odontogenic Differentiation ◽  
Human Dental Pulp ◽  
And Migration

Pentraxin-3 (PTX3) is recognized as a modulator of inflammation and a mediator of tissue repair. In this study, we characterized the role of PTX3 on some biological functions of human dental pulp stem cells (HDPSCs). The expression level of PTX3 significantly increased during osteogenic/odontogenic differentiation of HDPSCs, whereas the knockdown of PTX3 decreased this differentiation. Silencing of PTX3 in HDPSCs inhibited their migration and C-X-C chemokine receptor type 4 (CXCR4) expression. Our present study indicates that PTX3 is involved in osteogenic/odontogenic differentiation and migration of HDPSCs, and may contribute to the therapeutic potential of HDPSCs for regeneration and repair.

scholarly journals The effect of scaffold architecture on odontogenic differentiation of human dental pulp stem cells

Biomaterials ◽  
2011 ◽  
Vol 32 (31) ◽  
pp. 7822-7830 ◽  
Author(s):  
Jing Wang ◽  
Haiyun Ma ◽  
Xiaobing Jin ◽  
Jiang Hu ◽  
Xiaohua Liu ◽  
...  
Keyword(s):  
Stem Cells ◽  
Dental Pulp ◽  
Dental Pulp Stem Cells ◽  
Odontogenic Differentiation ◽  
Human Dental Pulp ◽  
Scaffold Architecture

scholarly journals miR-6807-5p Inhibited the Odontogenic Differentiation of Human Dental Pulp Stem Cells Through Directly Targeting METTL7A

2021 ◽  
Vol 9 ◽  
Author(s):  
Ning Wang ◽  
Xiao Han ◽  
Haoqing Yang ◽  
Dengsheng Xia ◽  
Zhipeng Fan
Keyword(s):  
Stem Cells ◽  
Dental Pulp ◽  
Binding Protein ◽  
Dental Pulp Stem Cells ◽  
Luciferase Reporter ◽  
Control Group ◽  
Alizarin Red ◽  
Odontogenic Differentiation ◽  
Human Dental Pulp ◽  
Tooth Tissue

Background: Tooth tissue regeneration mediated by mesenchymal stem cells (MSCs) has become the most ideal treatment. Although the known regulatory mechanism and some achievements have been discovered, directional differentiation cannot effectively induce regeneration of tooth tissue. In this study, we intended to explore the function and mechanism of miR-6807-5p and its target gene METTL7A in odontogenic differentiation.Methods: In this study, human dental pulp stem cells (DPSCs) were used. Alkaline phosphatase (ALP), Alizarin red staining (ARS), and calcium ion quantification were used to detect the odontogenic differentiation of miR-6807-5p and METTL7A. Real-time RT-PCR, western blot, dual-luciferase reporter assay, and pull-down assay with biotinylated miRNA were used to confirm that METTL7A was the downstream gene of miR-6807-5p. Protein mass spectrometry and co-immunoprecipitation (Co-IP) were used to detect that SNRNP200 was the co-binding protein of METTL7A.Results: After mineralized induction, the odontogenic differentiation was enhanced in the miR-6807-5p-knockdown group and weakened in the miR-6807-5p-overexpressed group compared with the control group. METTL7A was the downstream target of miR-6807-5p. After mineralized induction, the odontogenic differentiation was weakened in the METTL7A-knockdown group and enhanced in the METTL7A-overexpressed group compared with the control group. SNRNP200 was the co-binding protein of METTL7A. The knockdown of SNRNP200 inhibited the odontogenic differentiation of DPSCs.Conclusion: This study verified that miR-6807-5p inhibited the odontogenic differentiation of DPSCs. The binding site of miR-6807-5p was the 3′UTR region of METTL7A, which was silenced by miR-6807-5p. METTL7A promoted the odontogenic differentiation of DPSCs. SNRNP200, a co-binding protein of METTL7A, promoted the odontogenic differentiation of DPSCs.

scholarly journals The Conditioned Medium of Calcined Tooth Powder Promotes the Osteogenic and Odontogenic Differentiation of Human Dental Pulp Stem Cells via MAPK Signaling Pathways

2019 ◽  
Vol 2019 ◽  
pp. 1-13 ◽  
Author(s):  
Jintao Wu ◽  
Na Li ◽  
Yuan Fan ◽  
Yanqiu Wang ◽  
Yongchun Gu ◽  
...  
Keyword(s):  
Stem Cells ◽  
Signaling Pathways ◽  
Conditioned Medium ◽  
Dental Pulp ◽  
Mapk Signaling ◽  
Dental Pulp Stem Cells ◽  
Control Group ◽  
Odontogenic Differentiation ◽  
Human Dental Pulp ◽  
Mapk Signaling Pathways

The calcined tooth powder (CTP), a type of allogeneic biomimetic mineralized material, has been confirmed that can promote new bone formation when obtained at high temperature. The aim of this study was to investigate effects of the conditioned medium of calcined tooth powder (CTP-CM) on the osteogenic and odontogenic differentiation of human dental pulp stem cells (hDPSCs) and the underlying mechanisms involved. First, ALP activity assay determined that 200 μg/mL was the optimal concentration of CTP-CM for the following experiments. CTP-CM had no significant effect on the proliferation of hDPSCs as indicated by CCK-8 and FCM analysis. Both the gene and protein (DSPP/DSPP, RUNX2/RUNX2, OCN/OCN, OSX/OSX, OPN/OPN, ALP/ALP, and COL-1/COL-1) expression levels increased in the CTP-CM-induced hDPSC group as compared with those in the control group at day 3 or 7, showing the positive regulation of CTP-CM on the osteo/odontogenic differentiation of hDPSCs. Mechanistically, MAPK signaling pathways were activated after the CTP-CM treatment, and the inhibitors targeting MAPK were identified which weakened the effects of CTM-CM on the committed differentiation of hDPSCs. These findings could lead to the creation of stem cell therapies for dental regeneration.

scholarly journals The Effect of Mesoporous Bioactive Glass Nanoparticles/Graphene Oxide Composites on the Differentiation and Mineralization of Human Dental Pulp Stem Cells

Nanomaterials ◽  
2020 ◽  
Vol 10 (4) ◽  
pp. 620 ◽  
Author(s):  
Jae Hwa Ahn ◽  
In-Ryoung Kim ◽  
Yeon Kim ◽  
Dong-Hyun Kim ◽  
Soo-Byung Park ◽  
...  
Keyword(s):  
Stem Cells ◽  
Graphene Oxide ◽  
Bioactive Glass ◽  
Dental Pulp ◽  
Dental Pulp Stem Cells ◽  
Mrna Levels ◽  
Alizarin Red ◽  
Differentiation Potential ◽  
Human Dental Pulp ◽  
Mesoporous Bioactive Glass

The purpose of this study was to investigate the effects of mesoporous bioactive glass nanoparticle (MBN)/graphene oxide (GO) composites on the mineralization ability and differentiation potential of human dental pulp stem cells (hDPSCs). MBN/GO composites were synthesized using the sol-gel method and colloidal processing to enhance the bioactivity and mechanical properties of MBN. Characterization using FESEM, XRD, FTIR, and Raman spectrometry showed that the composites were successfully synthesized. hDPSCs were then cultured directly on the MBN/GO (40:1 and 20:1) composites in vitro. MBN/GO promoted the proliferation and alkaline phosphatase (ALP) activity of hDPSCs. In addition, qRT-PCR showed that MBN/GO regulated the mRNA levels of odontogenic markers (dentin sialophosphoprotein (DSPP), dentine matrix protein 1 (DMP-1), ALP, matrix extracellular phosphoglycoprotein (MEPE), bone morphogenetic protein 2 (BMP-2), and runt-related transcription factor 2 (RUNX-2)). The mRNA levels of DSPP and DMP-1, two odontogenesis-specific markers, were considerably upregulated in hDPSCs in response to growth on the MBN/GO composites. Western blot analysis revealed similar results. Alizarin red S staining was subsequently performed to further investigate MBN/GO-induced mineralization of hDPSCs. It was revealed that MBN/GO composites promote odontogenic differentiation via the Wnt/β-catenin signaling pathway. Collectively, the results of the present study suggest that MBN/GO composites may promote the differentiation of hDPSCs into odontoblast-like cells, and potentially induce dentin formation.

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