scholarly journals Optical switching of protein interactions on photosensitive–electroactive polymers measured by atomic force microscopy

2013 ◽  
Vol 1 (16) ◽  
pp. 2162 ◽  
Author(s):  
Amy Gelmi ◽  
Michele Zanoni ◽  
Michael J. Higgins ◽  
Sanjeev Gambhir ◽  
David L. Officer ◽  
...  
Keyword(s):  
Atomic Force Microscopy ◽  
Protein Interactions ◽  
Optical Switching ◽  
Electroactive Polymers ◽  
Force Microscopy ◽  
Atomic Force

Real-Time Observation of Phospholipid Bilayer Membrane Restructuring Induced by Protein Molecules using Atomic Force Microscopy

2001 ◽  
Vol 7 (S2) ◽  
pp. 858-859
Author(s):  
Hong Xing You ◽  
Xiaoyang Qi ◽  
Lei Yu
Keyword(s):  
Atomic Force Microscopy ◽  
Real Time ◽  
Lipid Bilayer ◽  
Protein Interactions ◽  
Phospholipid Bilayer ◽  
Biological Processes ◽  
Lipid Bilayer Membranes ◽  
Bilayer Membranes ◽  
Force Microscopy ◽  
Atomic Force

Atomic force microscopy (AFM) allows the surfaces of native biological materials to be imaged in aqueous solution with submolecular resolution. The ability to perform AFM imaging in aqueous and physiological environment has made it possible to monitor important biological processes in real time at high resolution. Currently, there is a great deal of interest in AFM studies of the structure and property of lipid bilayer membranes and protein interactions with lipid bilayer membranes. Lipid bilayer membranes in biological cells form a permeability barrier, which controls the flow of ions, water, and other molecules between biological cells and their environments, whereas membrane-bound and/or membrane-associated proteins are responsible for most of the dynamic functions carried out by the membrane. However, real-time AFM monitoring of dynamic biological processes has been challenged by the limited temporal resolution of AFM, potential physical damage to soft biological samples, and intrinsic complexity of biological processes. There are few successful examples of AFM real-time studies of dynamic biological events, particularly in the aspect of protein interactions with lipid bilayer membranes.We have attempted to use atomic force microscopy to study interactions between a particular protein, saposin C, and phospholipid bilayer membranes in real time. Saposin C (Sap C), a small glycoprotein, is an essential co-factor for the hydrolysis of glucosylceramide by glucosylceramidase in lysosomes, and a deficiency of Sap C leads to a variant form of Gauchers’ diseases. Supported planar phospholipid bilayer membranes were used in the study.

scholarly journals PEGylated surfaces for the study of DNA–protein interactions by atomic force microscopy

Nanoscale ◽  
2019 ◽  
Vol 11 (42) ◽  
pp. 20072-20080
Author(s):  
Bernice Akpinar ◽  
Philip J. Haynes ◽  
Nicholas A. W. Bell ◽  
Katharina Brunner ◽  
Alice L. B. Pyne ◽  
...  
Keyword(s):  
Atomic Force Microscopy ◽  
Protein Interactions ◽  
Polymer Surfaces ◽  
Block Polymer ◽  
Force Microscopy ◽  
Atomic Force ◽  
Dna Protein Interactions

Co-block polymer surfaces provide a platform on which to visualize DNA–protein interactions by atomic force microscopy at nanometre resolution.

Specific DNA−Protein Interactions on Mica Investigated by Atomic Force Microscopy

Langmuir ◽  
2010 ◽  
Vol 26 (4) ◽  
pp. 2618-2623 ◽  
Author(s):  
David Pastré ◽  
Loïc Hamon ◽  
Isabelle Sorel ◽  
Eric Le Cam ◽  
Patrick A. Curmi ◽  
...  
Keyword(s):  
Atomic Force Microscopy ◽  
Protein Interactions ◽  
Force Microscopy ◽  
Atomic Force ◽  
Dna Protein Interactions

Dynamic imaging of single DNA–protein interactions using atomic force microscopy

2003 ◽  
Vol 479 (1) ◽  
pp. 3-15 ◽  
Author(s):  
Martin L. Bennink ◽  
Dessy N. Nikova ◽  
Kees O. van der Werf ◽  
Jan Greve
Keyword(s):  
Atomic Force Microscopy ◽  
Protein Interactions ◽  
Dynamic Imaging ◽  
Force Microscopy ◽  
Atomic Force ◽  
Dna Protein Interactions

scholarly journals High-Speed Atomic Force Microscopy of Protein-Protein Interactions

2013 ◽  
Vol 104 (2) ◽  
pp. 386a
Author(s):  
Simon Scheuring ◽  
Ignacio Casuso ◽  
Felix Rico ◽  
Adai Colom
Keyword(s):  
Atomic Force Microscopy ◽  
Protein Interactions ◽  
High Speed ◽  
Protein Protein Interactions ◽  
Force Microscopy ◽  
Atomic Force
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