Imaging lipid order changes in endosome membranes of live cells by using a Nile Red-based membrane probe

RSC Advances ◽  
2014 ◽  
Vol 4 (17) ◽  
pp. 8481-8488 ◽  
Author(s):  
Zeinab Darwich ◽  
Andrey S. Klymchenko ◽  
Denis Dujardin ◽  
Yves Mély
Keyword(s):  
Nile Red ◽  
Live Cells ◽  
Lipid Order ◽  
Membrane Probe

Changes in the composition of endosome membranes during endocytosis can be imaged in live cells with the NR12S membrane probe.

Location, dynamics and solvent relaxation of a nile red-based phase-sensitive fluorescent membrane probe

2014 ◽  
Vol 183 ◽  
pp. 1-8 ◽  
Author(s):  
Roopali Saxena ◽  
Sandeep Shrivastava ◽  
Sourav Haldar ◽  
Andrey S. Klymchenko ◽  
Amitabha Chattopadhyay
Keyword(s):  
Nile Red ◽  
Solvent Relaxation ◽  
Membrane Probe ◽  
Phase Sensitive

scholarly journals Lipid Order Investigations Combined with Generalized Polarization Provide Deeper Insights into Plasma Membrane Architecture of Live Cells

2014 ◽  
Vol 106 (2) ◽  
pp. 510a
Author(s):  
Alla Kress ◽  
Julien Savatier ◽  
Xiao Wang ◽  
Patrick Ferrand ◽  
Sophie Brasselet
Keyword(s):  
Plasma Membrane ◽  
Live Cells ◽  
Generalized Polarization ◽  
Lipid Order ◽  
Membrane Architecture

scholarly journals Selective Staining and Quantification of Intracellular Lipid Droplets with CBD-Fluor, a New and Versatile Push-Pull Fluorophore

2019 ◽  
Author(s):  
S. Israel Suarez ◽  
Caroline C. Warner ◽  
Heather Brown-Harding ◽  
andrea thooft ◽  
Brett VanVeller ◽  
...  
Keyword(s):  
Lipid Droplets ◽  
Stokes Shift ◽  
Nile Red ◽  
Live Cells ◽  
Intracellular Lipid ◽  
Selective Staining ◽  
Large Stokes Shift ◽  
Fluorescent Stain ◽  
Photo Stability

A novel lipophilic dye, based on the structures of benzothiadiazole heterocycles, was shown to be an effective fluorescent stain for the imaging of lipid droplets (LDs) within both fixed and live cells. Its high photo stability, large Stokes shift, and improved staining and specificity over that of commercial dye Nile Red highlight its great potential as a versatile chemical tool for facilitating LD imaging and research.

Solvatochromic Nile Red Probes with FRET Quencher Reveal Lipid Order Heterogeneity in Living and Apoptotic Cells

2015 ◽  
Vol 10 (6) ◽  
pp. 1435-1442 ◽  
Author(s):  
Rémy Kreder ◽  
Kyrylo A. Pyrshev ◽  
Zeinab Darwich ◽  
Oleksandr A. Kucherak ◽  
Yves Mély ◽  
...  
Keyword(s):  
Nile Red ◽  
Apoptotic Cells ◽  
Lipid Order

scholarly journals Selective Staining and Quantification of Intracellular Lipid Droplets with CBD-Fluor, a New and Versatile Push-Pull Fluorophore

2019 ◽  
Author(s):  
S. Israel Suarez ◽  
Caroline C. Warner ◽  
Heather Brown-Harding ◽  
andrea thooft ◽  
Brett VanVeller ◽  
...  
Keyword(s):  
Lipid Droplets ◽  
Stokes Shift ◽  
Nile Red ◽  
Live Cells ◽  
Intracellular Lipid ◽  
Selective Staining ◽  
Large Stokes Shift ◽  
Fluorescent Stain ◽  
Photo Stability

A novel lipophilic dye, based on the structures of benzothiadiazole heterocycles, was shown to be an effective fluorescent stain for the imaging of lipid droplets (LDs) within both fixed and live cells. Its high photo stability, large Stokes shift, and improved staining and specificity over that of commercial dye Nile Red highlight its great potential as a versatile chemical tool for facilitating LD imaging and research.

Switchable Nile Red-Based Probe for Cholesterol and Lipid Order at the Outer Leaflet of Biomembranes

2010 ◽  
Vol 132 (13) ◽  
pp. 4907-4916 ◽  
Author(s):  
Oleksandr A. Kucherak ◽  
Sule Oncul ◽  
Zeinab Darwich ◽  
Dmytro A. Yushchenko ◽  
Youri Arntz ◽  
...  
Keyword(s):  
Nile Red ◽  
Lipid Order ◽  
Outer Leaflet

4-dimensional, high-resolution imaging of living cells

1992 ◽  
Vol 50 (1) ◽  
pp. 416-417
Author(s):  
Shinya Inoué
Keyword(s):  
Light Microscope ◽  
Living Cells ◽  
Live Cells ◽  
Video Tape ◽  
Active Living ◽  
High Resolution Imaging ◽  
3 Dimensional ◽  
Dynamic Architecture ◽  
Resolution Imaging ◽  
Disk Memory

This paper reports progress of our effort to rapidly capture, and display in time-lapsed mode, the 3-dimensional dynamic architecture of active living cells and developing embryos at the highest resolution of the light microscope. Our approach entails: (A) real-time video tape recording of through-focal, ultrathin optical sections of live cells at the highest resolution of the light microscope; (B) repeat of A at time-lapsed intervals; (C) once each time-lapsed interval, an image at home focus is recorded onto Optical Disk Memory Recorder (OMDR); (D) periods of interest are selected using the OMDR and video tape records; (E) selected stacks of optical sections are converted into plane projections representing different view angles (±4 degrees for stereo view, additional angles when revolving stereos are desired); (F) analysis using A - D.

En face dual fluorescence staining of fatty streaks allows observation of initiation and progression of atherosclerotic lesions

1995 ◽  
Vol 53 ◽  
pp. 864-865
Author(s):  
Anne M. Klinkner ◽  
Crystal R. Waites ◽  
Peter J. Bugelski ◽  
William D. Kerns
Keyword(s):  
Fluorescent Dyes ◽  
Nile Red ◽  
Dimethyl Formamide ◽  
High Cholesterol Diet ◽  
Methods Development ◽  
Atherosclerotic Lesions ◽  
En Face ◽  
Biochemical Evaluation ◽  
Stock Solutions ◽  
Dual Staining

A primary effort in the understanding of the progression of atherosclerotic disease has been methods development for visualization of the atherosclerotic plaque. We introduce a new method for the qualitative analysis of lipids in atherosclerotic fatty streaks which also retains those lipids for biochemical evaluation. An original aspect of the process is the ability to view an entire fatty streak en face, selectively stained for specific lipid classes within the lesion.New Zealand white rabbits were fed a high cholesterol diet(0.15%-0.3% for 14 wks). The aorta was removed and fixed in Carson's phosphate buffered formaldehyde followed by dual staining in the fluorescent dyes Nile red and filipin. Stock solutions of nile red(0.5mg/ml acetone) and filipin(2.5mg/ml dimethyl formamide) were prepared and kept at -20°C; all subsequent steps were at RT. 0.5cm × 1.0cm pieces of aorta were trimmed and adventitia removed. The pieces were then washed 3×15 min in PBS w/o CaMg, soaked in Nile red(NR)/filipin(Fl) stain(100(il NR stock + 200μl Fl stock in 10 ml PBS for 30 min, washed in PBS 3×30 min, rinsed with distilled water, mounted(Crystal Mount, Biomedia) and coverslipped and viewed by fluorescence microscopy.

Multi-mode light microscopy of microtubule assembly dynamics and chromosome movement in vivo and In vitro

1996 ◽  
Vol 54 ◽  
pp. 730-731
Author(s):  
E. D. Salmon ◽  
J. C. Waters ◽  
C. Waterman-Storer
Keyword(s):  
Imaging System ◽  
Ccd Camera ◽  
Transmitted Light ◽  
Microtubule Assembly ◽  
Live Cells ◽  
Optical Efficiency ◽  
Multiple Band ◽  
Multi Mode

We have developed a multi-mode digital imaging system which acquires images with a cooled CCD camera (Figure 1). A multiple band pass dichromatic mirror and robotically controlled filter wheels provide wavelength selection for epi-fluorescence. Shutters select illumination either by epi-fluorescence or by transmitted light for phase contrast or DIC. Many of our experiments involve investigations of spindle assembly dynamics and chromosome movements in live cells or unfixed reconstituted preparations in vitro in which photodamage and phototoxicity are major concerns. As a consequence, a major factor in the design was optical efficiency: achieving the highest image quality with the least number of illumination photons. This principle applies to both epi-fluorescence and transmitted light imaging modes. In living cells and extracts, microtubules are visualized using X-rhodamine labeled tubulin. Photoactivation of C2CF-fluorescein labeled tubulin is used to locally mark microtubules in studies of microtubule dynamics and translocation. Chromosomes are labeled with DAPI or Hoechst DNA intercalating dyes.

Characterization of a Motile Cellular Domain Arising During the Amoebo-Flagellate Transformation of Physarum Polycephalum

1980 ◽  
Vol 38 ◽  
pp. 552-553
Author(s):  
K.I. Pagh ◽  
M.R. Adelman
Keyword(s):  
Cell Shape ◽  
Physarum Polycephalum ◽  
Slime Mold ◽  
Live Cells ◽  
Cellular Domain

Unicellular amoebae of the slime mold Physarum polycephalum undergo marked changes in cell shape and motility during their conversion into flagellate swimming cells (l). To understand the processes underlying motile activities expressed during the amoebo-flagellate transformation, we have undertaken detailed investigations of the organization, formation and functions of subcellular structures or domains of the cell which are hypothesized to play a role in movement. One focus of our studies is on a structure, termed the “ridge” which appears as a flattened extension of the periphery along the length of transforming cells (Fig. 1). Observations of live cells using Nomarski optics reveal two types of movement in this region:propagation of undulations along the length of the ridge and formation and retraction of filopodial projections from its edge. The differing activities appear to be associated with two characteristic morphologies, illustrated in Fig. 1.

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