Reaction-based probes for Co(ii) and Cu(i) with dual output modes: fluorescence live cell imaging

RSC Advances ◽  
2013 ◽  
Vol 3 (37) ◽  
pp. 16788 ◽  
Author(s):  
Debabrata Maity ◽  
Anand Raj ◽  
D. Karthigeyan ◽  
Tapas K. Kundu ◽  
T. Govindaraju
Keyword(s):  
Live Cell Imaging ◽  
Cell Imaging ◽  
Live Cell ◽  
Fluorescence Live Cell Imaging

scholarly journals “Probe, Sample, and Instrument (PSI)”: The Hat-Trick for Fluorescence Live Cell Imaging

Chemosensors ◽  
2018 ◽  
Vol 6 (3) ◽  
pp. 40 ◽  
Author(s):  
Ludovic Galas ◽  
Thibault Gallavardin ◽  
Magalie Bénard ◽  
Arnaud Lehner ◽  
Damien Schapman ◽  
...  
Keyword(s):  
Organic Synthesis ◽  
Fluorescent Probes ◽  
Live Cell Imaging ◽  
Cell Imaging ◽  
Live Cell ◽  
Cell Labeling ◽  
Key Points ◽  
Research Infrastructures ◽  
New Strategies ◽  
Fluorescence Live Cell Imaging

Cell Imaging Platforms (CIPs) are research infrastructures offering support to a number of scientific projects including the choice of adapted fluorescent probes for live cell imaging. What to detect in what type of sample and for how long is a major issue with fluorescent probes and, for this, the “hat-trick” “Probe–Sample–Instrument” (PSI) has to be considered. We propose here to deal with key points usually discussed in CIPs including the properties of fluorescent organic probes, the modality of cell labeling, and the best equipment to obtain appropriate spectral, spatial, and temporal resolution. New strategies in organic synthesis and click chemistry for accessing probes with enhanced photophysical characteristics and targeting abilities will also be addressed. Finally, methods for image processing will be described to optimize exploitation of fluorescence signals.

scholarly journals TADF Dye-Loaded Nanoparticles for Fluorescence Live-Cell Imaging

2020 ◽  
Vol 8 ◽  
Author(s):  
Carina I. C. Crucho ◽  
João Avó ◽  
Ana M. Diniz ◽  
Sandra N. Pinto ◽  
José Barbosa ◽  
...  
Keyword(s):  
Live Cell Imaging ◽  
Cell Imaging ◽  
Live Cell ◽  
Fluorescence Live Cell Imaging

Analysis of the influence of subcellular localization of the HIV Rev protein on Rev-dependent gene expression by multi-fluorescence live-cell imaging

2006 ◽  
Vol 312 (4) ◽  
pp. 443-456 ◽  
Author(s):  
Horst Wolff ◽  
Kamyar Hadian ◽  
Manja Ziegler ◽  
Claudia Weierich ◽  
Susanne Kramer-Hammerle ◽  
...  
Keyword(s):  
Gene Expression ◽  
Subcellular Localization ◽  
Live Cell Imaging ◽  
Cell Imaging ◽  
Live Cell ◽  
Rev Protein ◽  
Fluorescence Live Cell Imaging

Concentration-dependent fluorescence live-cell imaging and tracking of intracellular nanoparticles

2011 ◽  
Vol 22 (23) ◽  
pp. 235101 ◽  
Author(s):  
Ji Hye Seo ◽  
Keunchang Cho ◽  
So Yeong Lee ◽  
Sang-Woo Joo
Keyword(s):  
Live Cell Imaging ◽  
Cell Imaging ◽  
Live Cell ◽  
Fluorescence Live Cell Imaging

Fluorescence Live Cell Imaging Revealed Wogonin Targets Mitochondria

Talanta ◽  
2021 ◽  
pp. 122328
Author(s):  
Su Liang ◽  
Zeyu Wang ◽  
Luyu Qi ◽  
Chao Tang ◽  
Yiming Zhang ◽  
...  
Keyword(s):  
Live Cell Imaging ◽  
Cell Imaging ◽  
Live Cell ◽  
Fluorescence Live Cell Imaging

Enhanced 3D fluorescence live cell imaging on nanoplasmonic substrate

2011 ◽  
Vol 22 (36) ◽  
pp. 365203 ◽  
Author(s):  
Manas Ranjan Gartia ◽  
Austin Hsiao ◽  
Mayandi Sivaguru ◽  
Yi Chen ◽  
G Logan Liu
Keyword(s):  
Live Cell Imaging ◽  
Cell Imaging ◽  
Live Cell ◽  
Fluorescence Live Cell Imaging

scholarly journals Host defence peptides identified in human apolipoprotein B as promising antifungal agents

2021 ◽  
Vol 105 (5) ◽  
pp. 1953-1964
Author(s):  
Eliana Dell’Olmo ◽  
Rosa Gaglione ◽  
Angela Cesaro ◽  
Valeria Cafaro ◽  
Wieke R. Teertstra ◽  
...  
Keyword(s):  
Apolipoprotein B ◽  
Live Cell Imaging ◽  
Cell Imaging ◽  
Fungicidal Activity ◽  
Antifungal Agents ◽  
Live Cell ◽  
List Type ◽  
Human Apolipoprotein ◽  
Wide Range ◽  
Fluorescence Live Cell Imaging

Abstract Therapeutic options to treat invasive fungal infections are still limited. This makes the development of novel antifungal agents highly desirable. Naturally occurring antifungal peptides represent valid candidates, since they are not harmful for human cells and are endowed with a wide range of activities and their mechanism of action is different from that of conventional antifungal drugs. Here, we characterized for the first time the antifungal properties of novel peptides identified in human apolipoprotein B. ApoB-derived peptides, here named r(P)ApoBLPro, r(P)ApoBLAla and r(P)ApoBSPro, were found to have significant fungicidal activity towards Candida albicans (C. albicans) cells. Peptides were also found to be able to slow down metabolic activity of Aspergillus niger (A. niger) spores. In addition, experiments were carried out to clarify the mechanism of fungicidal activity of ApoB-derived peptides. Peptides immediately interacted with C. albicans cell surfaces, as indicated by fluorescence live cell imaging analyses, and induced severe membrane damage, as indicated by propidium iodide uptake induced upon treatment of C. albicans cells with ApoB-derived peptides. ApoB-derived peptides were also tested on A. niger swollen spores, initial hyphae and branched mycelium. The effects of peptides were found to be more severe on swollen spores and initial hyphae compared to mycelium. Fluorescence live cell imaging analyses confirmed peptide internalization into swollen spores with a consequent accumulation into hyphae. Altogether, these findings open interesting perspectives to the application of ApoB-derived peptides as effective antifungal agents. Key points Human cryptides identified in ApoB are effective antifungal agents. ApoB-derived cryptides exert fungicidal effects towards C. albicans cells. ApoB-derived cryptides affect different stages of growth of A. niger. Graphical abstract

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