New meso-substituted trans-A2B2 di(4-pyridyl)porphyrins as building blocks for metal-mediated self-assembling of 4 + 4 Re(i)–porphyrin metallacycles

Mariangela Boccalon; Elisabetta Iengo; Paolo Tecilla

doi:10.1039/c3ob40452h

Combinatorial Peptide Libraries for Selecting Inorganic-Binding Proteins: A Step in Molecular Biomimetics

MRS Proceedings ◽

10.1557/proc-773-n8.1 ◽

2003 ◽

Vol 773 ◽

Cited By ~ 1

Author(s):

C. Tamerler ◽

S. Dinçer ◽

D. Heidel ◽

N. Karagûler ◽

M. Sarikaya

Keyword(s):

Binding Proteins ◽

Building Blocks ◽

Molecular Engineering ◽

Inorganic Materials ◽

Combinatorial Peptide Libraries ◽

Self Assembling ◽

Complex Functions ◽

Recent Developments ◽

Specific Proteins ◽

Molecular Biomimetics

AbstractProteins, one of the building blocks in organisms, not only control the assembly in biological systems but also provide most of their complex functions. It may be possible to assemble materials for practical technological applications utilizing the unique advantages provided by proteins. Here we discuss molecular biomimetic pathways in the quest for imitating biology at the molecular scale via protein engineering. We use combinatorial biology protocols to select short polypeptides that have affinity to inorganic materials and use them in assembling novel hybrid materials. We give an overview of some of the recent developments of molecular engineering towards this goal. Inorganic surface specific proteins were identified by using cell surface and phage display technologies. Examples of metal and metal oxide specific polypeptides were represented with an emphasis on certain level of specificities. The recognition and self assembling characteristics of these inorganic-binding proteins would be employed in develeopment of hybrid multifunctional materials for novel bio- and nano-technological applications.

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Factors Affecting Molecular Self-Assembly and Its Mechanism

Scientific Research Journal ◽

10.24191/srj.v9i1.5385 ◽

2012 ◽

Vol 9 (1) ◽

pp. 43 ◽

Cited By ~ 1

Author(s):

Hueyling Tan

Keyword(s):

Self Assembly ◽

Building Blocks ◽

Molecular Engineering ◽

Molecular Structures ◽

Polymer Science ◽

New Approach ◽

Factors Affecting ◽

Dna Structures ◽

Self Assembling ◽

Wide Range

Molecular self-assembly is ubiquitous in nature and has emerged as a new approach to produce new materials in chemistry, engineering, nanotechnology, polymer science and materials. Molecular self-assembly has been attracting increasing interest from the scientific community in recent years due to its importance in understanding biology and a variety of diseases at the molecular level. In the last few years, considerable advances have been made in the use ofpeptides as building blocks to produce biological materials for wide range of applications, including fabricating novel supra-molecular structures and scaffolding for tissue repair. The study ofbiological self-assembly systems represents a significant advancement in molecular engineering and is a rapidly growing scientific and engineering field that crosses the boundaries ofexisting disciplines. Many self-assembling systems are rangefrom bi- andtri-block copolymers to DNA structures as well as simple and complex proteins andpeptides. The ultimate goal is to harness molecular self-assembly such that design andcontrol ofbottom-up processes is achieved thereby enabling exploitation of structures developed at the meso- and macro-scopic scale for the purposes oflife and non-life science applications. Such aspirations can be achievedthrough understanding thefundamental principles behind the selforganisation and self-synthesis processes exhibited by biological systems.

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Design and synthesis of macromolecular structures from self-assembling building blocks

Macromolecular Symposia ◽

10.1002/masy.19950980138 ◽

1995 ◽

Vol 98 (1) ◽

pp. 483-490

Author(s):

J. L. M. van Nunen ◽

A. P. H. J. Schenning ◽

R. J. H. Hafkamp ◽

C. F. van Nostrum ◽

M. C. Feiters ◽

...

Keyword(s):

Building Blocks ◽

Design And Synthesis ◽

Self Assembling

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De novo design of self-assembling helical protein filaments

Science ◽

10.1126/science.aau3775 ◽

2018 ◽

Vol 362 (6415) ◽

pp. 705-709 ◽

Cited By ~ 48

Author(s):

Hao Shen ◽

Jorge A. Fallas ◽

Eric Lynch ◽

William Sheffler ◽

Bradley Parry ◽

...

Keyword(s):

De Novo ◽

Computational Design ◽

Building Blocks ◽

Repeat Units ◽

Self Assembling ◽

Wide Range ◽

Helical Protein ◽

Monomeric Proteins

We describe a general computational approach to designing self-assembling helical filaments from monomeric proteins and use this approach to design proteins that assemble into micrometer-scale filaments with a wide range of geometries in vivo and in vitro. Cryo–electron microscopy structures of six designs are close to the computational design models. The filament building blocks are idealized repeat proteins, and thus the diameter of the filaments can be systematically tuned by varying the number of repeat units. The assembly and disassembly of the filaments can be controlled by engineered anchor and capping units built from monomers lacking one of the interaction surfaces. The ability to generate dynamic, highly ordered structures that span micrometers from protein monomers opens up possibilities for the fabrication of new multiscale metamaterials.

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Spontaneous Registration of Sub-10 nm Features Based on Subzero Celsius Spin-Casting of Self-Assembling Building Blocks Directed by Chemically Encoded Surfaces

ACS Nano ◽

10.1021/acsnano.8b03378 ◽

2018 ◽

Vol 12 (8) ◽

pp. 8224-8233 ◽

Cited By ~ 3

Author(s):

Jung Hye Lee ◽

Hak-Jong Choi ◽

ChulHee Lee ◽

Seung Won Song ◽

Joong Bum Lee ◽

...

Keyword(s):

Building Blocks ◽

Spin Casting ◽

Self Assembling

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Myosins generate contractile force and maintain organization in the cytokinetic contractile ring

10.1101/2021.05.02.442363 ◽

2021 ◽

Author(s):

Zachary A. McDargh ◽

Shuyuan Wang ◽

Harvey F. Chin ◽

Sathish Thiyagarajan ◽

Erdem Karatekin ◽

...

Keyword(s):

Fission Yeast ◽

Striated Muscle ◽

Protein Complexes ◽

Myosin Ii ◽

Force Production ◽

Super Resolution ◽

Building Blocks ◽

Contractile Ring ◽

Ring Model ◽

Self Assembling

During cytokinesis, cells assemble an actomyosin contractile ring whose tension constricts and divides cells, but the ring tension was rarely measured. Actomyosin force generation is well understood for the regular sarcomeric architecture of striated muscle, but recent super-resolution studies of fission yeast contractile rings revealed organizational building blocks that are not sarcomeres but irregularly positioned plasma membrane-anchored protein complexes called nodes. Here, we measured contractile ring tensions in fission yeast protoplast cells. The myosin II isoforms Myo2 and Myp2 generated the tension, with a ~2-fold greater contribution from Myo2. Simulations of a molecularly detailed ring model revealed a sliding node mechanism for tension, where nodes hosting tense actin filaments were pulled bidirectionally around the ring. Myo2 and Myp2 chaperoned self-assembling components into the ring organization, and anchored the ring against bridging instabilities. Thus, beyond force production, Myo2 and Myp2 are the principal organizers, bundlers and anchors of the contractile ring.

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Hierarchical structures via self-assembling protein-polymer hybrid building blocks

Polymer ◽

10.1016/j.polymer.2012.10.054 ◽

2012 ◽

Vol 53 (26) ◽

pp. 6045-6052 ◽

Cited By ~ 14

Author(s):

Patrick van Rijn ◽

Nathalie C. Mougin ◽

Alexander Böker

Keyword(s):

Hierarchical Structures ◽

Building Blocks ◽

Protein Polymer ◽

Self Assembling ◽

Polymer Hybrid

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Coiled-Coils: The Molecular Zippers that Self-Assemble Protein Nanostructures

International Journal of Molecular Sciences ◽

10.3390/ijms21103584 ◽

2020 ◽

Vol 21 (10) ◽

pp. 3584 ◽

Cited By ~ 1

Author(s):

Won Min Park

Keyword(s):

Modular Design ◽

Coiled Coil ◽

Molecular Complexes ◽

Building Blocks ◽

Structural Features ◽

Coiled Coils ◽

Design Strategies ◽

Protein Motifs ◽

Self Assembling ◽

Current Design

Coiled-coils, the bundles of intertwined helical protein motifs, have drawn much attention as versatile molecular toolkits. Because of programmable interaction specificity and affinity as well as well-established sequence-to-structure relationships, coiled-coils have been used as subunits that self-assemble various molecular complexes in a range of fields. In this review, I describe recent advances in the field of protein nanotechnology, with a focus on programming assembly of protein nanostructures using coiled-coil modules. Modular design approaches to converting the helical motifs into self-assembling building blocks are described, followed by a discussion on the molecular basis and principles underlying the modular designs. This review also provides a summary of recently developed nanostructures with a variety of structural features, which are in categories of unbounded nanostructures, discrete nanoparticles, and well-defined origami nanostructures. Challenges existing in current design strategies, as well as desired improvements for controls over material properties and functionalities for applications, are also provided.

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