Arsinous acid as a thiol binding group: potential cysteine peptide tagging functionality that binds a single thiol

2014 ◽  
Vol 38 (4) ◽  
pp. 1368-1371 ◽  
Author(s):  
Xiaofei Liang ◽  
Dale G. Drueckhammer
Keyword(s):  
Silver Salt ◽  
Thiol Binding

A simple aryl arsinous acid (ArAs(CH3)OH) was prepared byorthomercuration ofp-cresol followed by Pd-catalyzed reaction with methylarsenic dibromide, purification as the mercaptoethanol adduct, and deprotection using a silver salt.

The Silver Salt of 12-Tungstophosphoric Acid: An Efficient Catalyst for the Three-Component Coupling of an Aldehyde, an Amine and an Alkyne.

ChemInform ◽  
2007 ◽  
Vol 38 (6) ◽  
Author(s):  
K. Mohan Reddy ◽  
N. Seshu Babu ◽  
I. Suryanarayana ◽  
P. S. Sai Prasad ◽  
N. Lingaiah
Keyword(s):  
Silver Salt ◽  
Tungstophosphoric Acid ◽  
Efficient Catalyst ◽  
Three Component Coupling

Replacing a stoichiometric silver oxidant with air: ligated Pd(ii)-catalysis to β-aryl carbonyl derivatives with improved chemoselectivity

2014 ◽  
Vol 16 (5) ◽  
pp. 2788-2797 ◽  
Author(s):  
Mari Vellakkaran ◽  
Murugaiah M. S. Andappan ◽  
Nagaiah Kommu
Keyword(s):  
Silver Salt ◽  
Carbonyl Derivatives

Air was employed as a green reoxidant of Pd(0), replacing stoichiometric and toxic silver salt, in the chelation-controlled Pd(ii)-modulated arylative enolization of prop-2-en-1-ols to acquire synthetically-important β-aryl carbonyl derivatives.

scholarly journals Localization of three non-thiol binding sites on polypeptide chain β yeast fatty acid synthetase

FEBS Letters ◽  
1977 ◽  
Vol 82 (1) ◽  
pp. 139-142 ◽  
Author(s):  
Hansjörg Engeser ◽  
Felix Wieland ◽  
Feodor Lynen
Keyword(s):  
Fatty Acid ◽  
Binding Sites ◽  
Polypeptide Chain ◽  
Fatty Acid Synthetase ◽  
Thiol Binding

Energy coupling mechanisms in host-grown Mycobacterium lepraemurium

1981 ◽  
Vol 59 (2) ◽  
pp. 75-82 ◽  
Author(s):  
M. Ishaque ◽  
C. Adapoe ◽  
L. Kato
Keyword(s):  
Energy Coupling ◽  
Oxidative Enzymes ◽  
Sprague Dawley ◽  
Succinate Oxidation ◽  
Sprague Dawley Rats ◽  
Mycobacterium Lepraemurium ◽  
Thiol Binding ◽  
Coupling Mechanisms ◽  
Respiratory Chain Inhibitors

Energy coupling mechanisms of Mycobacterium lepraemurium isolated from Sprague-Dawley rats lepromata were investigated. Cell-free extracts catalyzed phosphorylation coupled to the oxidation of generated NADH, added NADH, and succinate yielding P/O ratios of approximately 0.8, 0.6, and 0.4, respectively. Ascorbate oxidation alone or in the presence of cytochrome c or N,N,N′,N′-tetramethyl-p-phenylenediamine was not coupled to ATP synthesis.The oxidative phosphorylation was completely uncoupled by 2,4-dinitrophenol, 2,6-dibromophenol, pentachlorophenol, m-chlorocarbonylcyanide phenylhydrazone, dicumarol, and gramicidin at concentrations which did not cause any inhibition of oxygen uptake. While the NADH oxidation and associated phosphate esterification was markedly sensitive to rotenone and other flavoprotein inhibitors, these inhibitors had no effect, however, on the phosphorylation coupled to succinate oxidation. The respiratory chain inhibitors such as antimycin A or 2-n-heptyl-4-hydroxyquinoline-N-oxide, and cyanide were the potent inhibitors of the phosphorylation associated with the oxidation of NADH and succinate. The ATP formation coupled to the oxidation of NADH and succinate was also inhibited by oligomycin as well as by the thiol-binding agents, p-hydroxymercuribenzoate and N-ethylmaleimide. The results indicated that NADH and succinate oxidation by in vivo grown M. lepraemurium was mediated by oxidative enzymes involving first and second energy coupling sites.

scholarly journals Sandwich-Type DNA Micro-Optode Based on Gold–Latex Spheres Label for Reflectance Dengue Virus Detection

Sensors ◽  
2020 ◽  
Vol 20 (7) ◽  
pp. 1820
Author(s):  
Jeningsih ◽  
Ling Ling Tan ◽  
Alizar Ulianas ◽  
Lee Yook Heng ◽  
Nur-Fadhilah Mazlan ◽  
...  
Keyword(s):  
Dengue Virus ◽  
Limit Of Detection ◽  
Virus Detection ◽  
Average Diameter ◽  
Optical Biosensor ◽  
Clinical Samples ◽  
Sandwich Hybridization ◽  
Latex Spheres ◽  
Sandwich Type ◽  
Thiol Binding

A DNA micro-optode for dengue virus detection was developed based on the sandwich hybridization strategy of DNAs on succinimide-functionalized poly(n-butyl acrylate) (poly(nBA-NAS)) microspheres. Gold nanoparticles (AuNPs) with an average diameter of ~20 nm were synthesized using a centrifugation-based method and adsorbed on the submicrometer-sized polyelectrolyte-coated poly(styrene-co-acrylic acid) (PSA) latex particles via an electrostatic method. The AuNP–latex spheres were attached to the thiolated reporter probe (rDNA) by Au–thiol binding to functionalize as an optical gold–latex–rDNA label. The one-step sandwich hybridization recognition involved a pair of a DNA probe, i.e., capture probe (pDNA), and AuNP–PSA reporter label that flanked the target DNA (complementary DNA (cDNA)). The concentration of dengue virus cDNA was optically transduced by immobilized AuNP–PSA–rDNA conjugates as the DNA micro-optode exhibited a violet hue upon the DNA sandwich hybridization reaction, which could be monitored by a fiber-optic reflectance spectrophotometer at 637 nm. The optical genosensor showed a linear reflectance response over a wide cDNA concentration range from 1.0 × 10−21 M to 1.0 × 10−12 M cDNA (R2 = 0.9807) with a limit of detection (LOD) of 1 × 10−29 M. The DNA biosensor was reusable for three consecutive applications after regeneration with mild sodium hydroxide. The sandwich-type optical biosensor was well validated with a molecular reverse transcription polymerase chain reaction (RT-PCR) technique for screening of dengue virus in clinical samples, e.g., serum, urine, and saliva from dengue virus-infected patients under informed consent.

Olefin separation using silver impregnated ion-exchange membranes and silver salt/polymer blend membranes

1996 ◽  
Vol 117 (1-2) ◽  
pp. 151-161 ◽  
Author(s):  
Takeo Yamaguchi ◽  
Chelsey Baertsch ◽  
Carl A. Koval ◽  
Richard D. Noble ◽  
Christopher N. Bowman
Keyword(s):  
Ion Exchange ◽  
Polymer Blend ◽  
Silver Salt ◽  
Ion Exchange Membranes ◽  
Blend Membranes
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