Highly specific and sensitive detection of bisphenol A in water samples using an enzyme-linked immunosorbent assay employing a novel synthetic antigen

2014 ◽  
Vol 38 (2) ◽  
pp. 669-675 ◽  
Author(s):  
Wenbin Miao ◽  
Biwen Wei ◽  
Rongjing Yang ◽  
Chunhua Wu ◽  
Dan Lou ◽  
...  
Keyword(s):  
Bisphenol A ◽  
Immunosorbent Assay ◽  
Water Samples ◽  
Sensitive Detection ◽  
Enzyme Linked Immunosorbent Assay

Competitive plasmonic biomimetic enzyme-linked immunosorbent assay for sensitive detection of bisphenol A

2020 ◽  
pp. 128602
Author(s):  
Min Jia ◽  
Shuang Chen ◽  
Tingting Shi ◽  
Chunyang Li ◽  
Yanping Wang ◽  
...  
Keyword(s):  
Bisphenol A ◽  
Immunosorbent Assay ◽  
Sensitive Detection ◽  
Enzyme Linked Immunosorbent Assay

Development of a Direct Competitive Enzyme-linked Immunosorbent Assay Using a Sensitive Monoclonal Antibody for Bisphenol A

Hybridoma ◽  
2011 ◽  
Vol 30 (1) ◽  
pp. 95-100 ◽  
Author(s):  
Chunmei Ju ◽  
Youhua Xiong ◽  
Aizhong Gao ◽  
Tangbin Yang ◽  
Lei Wang
Keyword(s):  
Monoclonal Antibody ◽  
Bisphenol A ◽  
Immunosorbent Assay ◽  
Enzyme Linked Immunosorbent Assay

scholarly journals A rapid and highly sensitive biomarker detection platform based on a temperature-responsive liposome-linked immunosorbent assay

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Runkai Hu ◽  
Keitaro Sou ◽  
Shinji Takeoka
Keyword(s):  
Immunosorbent Assay ◽  
Limit Of Detection ◽  
Specific Antigen ◽  
Fluorescence Emission ◽  
Detection Sensitivity ◽  
Sensitive Detection ◽  
Enzyme Linked Immunosorbent Assay ◽  
Temperature Responsive ◽  
Highly Sensitive ◽  
Highly Sensitive Detection

Abstract The enzyme-linked immunosorbent assay (ELISA) is widely used in various fields to detect specific biomarkers. However, ELISA tests have limited detection sensitivity (≥ 1 pM), which is insufficiently sensitive for the detection of small amounts of biomarkers in the early stages of disease or infection. Herein, a method for the rapid and highly sensitive detection of specific antigens, using temperature-responsive liposomes (TLip) containing a squaraine dye that exhibits fluorescence at the phase transition temperature of the liposomes, was developed. A proof-of-concept study using biotinylated TLip and a streptavidin-immobilized microwell plate showed that the TLip bound to the plate via specific molecular recognition could be distinguished from unbound TLip within 1 min because of the difference in the heating time required for the fluorescence emission of TLip. This system could be used to detect prostate specific antigen (PSA) based on a sandwich immunosorbent assay using detection and capture antibodies, in which the limit of detection was as low as 27.6 ag/mL in a 100-μL PSA solution, 0.97 aM in terms of molar concentration. The present temperature-responsive liposome-linked immunosorbent assay provides an advanced platform for the rapid and highly sensitive detection of biomarkers for use in diagnosis and biological inspections.

scholarly journals Detection of Campylobacter jejuni and Campylobacter coli in Environmental Waters by PCR Enzyme-Linked Immunosorbent Assay

2002 ◽  
Vol 68 (3) ◽  
pp. 1319-1324 ◽  
Author(s):  
A. D. Sails ◽  
F. J. Bolton ◽  
A. J. Fox ◽  
D. R. A. Wareing ◽  
D. L. A. Greenway
Keyword(s):  
Campylobacter Jejuni ◽  
Immunosorbent Assay ◽  
Water Samples ◽  
Enrichment Culture ◽  
Environmental Water ◽  
Environmental Water Samples ◽  
Enzyme Linked Immunosorbent Assay ◽  
Campylobacter Coli ◽  
Elisa Assay ◽  
Bacterial Cells

ABSTRACT A PCR enzyme-linked immunosorbent assay (ELISA) assay was applied to the detection of Campylobacter jejuni and Campylobacter coli in environmental water samples after enrichment culture. Bacterial cells were concentrated from 69 environmental water samples by using filtration, and the filtrates were cultured in Campylobacter blood-free broth. After enrichment culture, DNA was extracted from the samples by using a rapid-boiling method, and the DNA extracts were used as a template in a PCR ELISA assay. A total of 51 samples were positive by either PCR ELISA or culture; of these, 43 were found to be positive by PCR ELISA and 43 were found to be positive by culture. Overall, including positive and negative results, 59 samples were concordant in both methods. Several samples were positive in the PCR ELISA assay but were culture negative; therefore, this assay may be able to detect sublethally damaged or viable nonculturable forms of campylobacters. The method is rapid and sensitive, and it significantly reduces the time needed for the detection of these important pathogens by 2 to 3 days.

Detection of bisphenol A in water samples using ELISA determination method

2011 ◽  
Vol 11 (1) ◽  
pp. 55-60 ◽  
Author(s):  
J. Zheng ◽  
S. Q. Zhao ◽  
X. T. Xu ◽  
K. Zhang
Keyword(s):  
Drinking Water ◽  
Bisphenol A ◽  
Water Samples ◽  
Limit Of Detection ◽  
Industrial Effluent ◽  
Cross Reactivity ◽  
Enzyme Linked Immunosorbent Assay ◽  
Uv Spectrophotometry ◽  
Inhibition Concentration

In order to study whether bisphenol A (BPA) can pass into drinking water from polycarbonate barrel and exist in the river and industrial effluent the indirect competitive enzyme-linked immunosorbent assay (ELISA) for the determination of BPA was established. The results presented an inhibition concentration at 50% absorbance (IC50) of 0.123 mg L−1, and the limit of detection (LOD) is 9.934 μg L−1. The specificity of antiserum was proved well because the cross-reactivity with benzene, tert-butylbenzene, hydroquinone and o-hydroxybenzoic acid were found lower than 0.01%, except phenol was 0.26%. The method was found to be reliable and repeatable. It was used for monitoring the concentration of BPA in the barreled drinking water. The results confirmed BPA can pass into barreled drinking water from the polycarbonate barrel and concentration increased as days went on. A certain content of BPA was found in industrial effluent. The results of ELISA were consistent with the results of UV spectrophotometry. BPA could not be found in the water samples obtained from Zhujiang River. The established method shows specific recognition of BPA and could be applied in detection of environmental BPA.

Sign in / Sign up

Export Citation Format

Share Document