High electron transfer capacity of thio-derivatives of tea catechins measured using a water soluble stable free radical and their effects on colon cancer cells

2013 ◽  
Vol 37 (7) ◽  
pp. 2043 ◽  
Author(s):  
Anna Carreras ◽  
Juan Antonio Mesa ◽  
Marta Cascante ◽  
Josep Lluís Torres ◽  
Luis Juliá
Keyword(s):  
Colon Cancer ◽  
Electron Transfer ◽  
Free Radical ◽  
Cancer Cells ◽  
Water Soluble ◽  
High Electron ◽  
Colon Cancer Cells ◽  
Stable Free Radical ◽  
Derivatives Of ◽  
Electron Transfer Capacity

scholarly journals Water Soluble Pleurotus ostreatus Polysaccharide Down-Regulates the Expression of MMP-2 and MMP-9 in Caco-2 Cells

2013 ◽  
Vol 41 (2) ◽  
pp. 553
Author(s):  
Sabina COJOCARU ◽  
Mihaela RADU ◽  
Liviu Gabriel BODEA ◽  
Mirela Mihaela CIMPEANU ◽  
Gogu GHEORGHITA ◽  
...  
Keyword(s):  
Colon Cancer ◽  
Cancer Cells ◽  
Protein Complexes ◽  
Gelatin Zymography ◽  
Water Soluble ◽  
Colon Cancer Cells ◽  
Sod Activity ◽  
Kinetic Measurements ◽  
The Third

Many polysaccharides and polysaccharide-protein complexes isolated from mushrooms have immunomodulatory and anti-cancer effects. Our aim was to study the regulatory mechanisms of Caco-2 cell response to water soluble P. ostreatus polysaccharide extract up to 72 hours. Specific enzymatic activities were assessed by kinetic measurements. The reduced glutathione content and the lipid peroxidation level were also analyzed. Protein expression of several heat shock proteins, Bcl-2 and metalloproteinases 2 and 9 were revealed by Western blot. Gelatin zymography assay was used to evaluate the MMP-2 and MMP-9 activities. Until the third day of exposure the total SOD activity decreased continuously by 30%, whereas GST and GR ones diminished by 17% respectively 30.5% compared to control. No significant changes were observed in CAT and G6PDH specific activities as well as in GSH and MDA concentration. After the third day of exposure a significant up-regulation of Hsp60 and Hsp90 expression and a down-regulation of Hsp70 one were registered. Bcl-2 protein levels were down-regulated by 50% in the first day of treatment but increased after 3 days. MMP-2 and 9 secretion in the culture medium was significantly reduced suggesting a diminished ability of invasion of colon cancer cells. Our data revealed that in vitro treatment with P. ostreatus aqueous polysaccharide extract does not induce apoptosis in Caco-2 cell line but it could inhibit the invasion of colon cancer cells through the basement membrane.

scholarly journals Carboranyl Derivatives of Rofecoxib with Cytostatic Activity against Human Melanoma and Colon Cancer Cells

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Antonio Buzharevski ◽  
Svetlana Paskaš ◽  
Menyhárt-Botond Sárosi ◽  
Markus Laube ◽  
Peter Lönnecke ◽  
...  
Keyword(s):  
Colon Cancer ◽  
Cancer Cells ◽  
Human Melanoma ◽  
Cytostatic Activity ◽  
Colon Cancer Cells ◽  
Derivatives Of

scholarly journals Chlorambucil Conjugated Ugi Dendrimers with PAMAM-NH2 Core and Evaluation of Their Anticancer Activity

Pharmaceutics ◽  
2019 ◽  
Vol 11 (2) ◽  
pp. 59 ◽  
Author(s):  
Nalin Seixas ◽  
Bruno Ravanello ◽  
Ibrahim Morgan ◽  
Goran Kaluđerović ◽  
Ludger Wessjohann
Keyword(s):  
Colon Cancer ◽  
Anticancer Activity ◽  
Cancer Cells ◽  
Water Soluble ◽  
Chemotherapeutic Drug ◽  
Colon Cancer Cells ◽  
Colon Cancer Cell Lines ◽  
M Phase ◽  
Ht 29 ◽  
Enhance Activity

Herein, a new Ugi multicomponent reaction strategy is described to enhance activity and solubility of the chemotherapeutic drug chlorambucil through its conjugation to poly(amidoamine) (PAMAM-NH2) dendrimers with the simultaneous introduction of lipidic (i-Pr) and cationic (–NH2) or anionic (–COOH) groups. Standard viability assays were used to evaluate the anticancer potential of the water-soluble dendrimers against PC-3 prostate and HT-29 colon cancer cell lines, as well as non-cancerous mouse NIH3T3 fibroblasts. It could be demonstrated that the anticancer activity against PC-3 cells was considerably improved when both chlorambucil and –NH2 (cationic) groups were present on the dendrimer surface (1b). Additionally, this dendrimer showed activity only against the prostate cancer cells (PC-3), while it did not affect colon cancer cells and fibroblasts significantly. The cationic chlorambucil-dendrimer 1b blocks PC-3 cells in the G2/M phase and induces caspase independent apoptosis.

scholarly journals The modulating effect of methoxy-derivatives of 2’-hydroxychalcones on the release of IL-8, MIF, VCAM-1 and ICAM-1 by colon cancer cells

2022 ◽  
Vol 145 ◽  
pp. 112428
Author(s):  
Joanna Bronikowska ◽  
Małgorzata Kłósek ◽  
Tomasz Janeczko ◽  
Edyta Kostrzewa-Susłow ◽  
Zenon P. Czuba
Keyword(s):  
Colon Cancer ◽  
Cancer Cells ◽  
Colon Cancer Cells ◽  
Derivatives Of

scholarly journals High folic acid increases cell turnover and lowers differentiation and iron content in human HT29 colon cancer cells

2007 ◽  
Vol 99 (4) ◽  
pp. 703-708 ◽  
Author(s):  
Linette Pellis ◽  
Yvonne Dommels ◽  
Dini Venema ◽  
Ab van Polanen ◽  
Esther Lips ◽  
...  
Keyword(s):  
Colon Cancer ◽  
Folic Acid ◽  
Cancer Cells ◽  
Folic Acid Supplementation ◽  
Water Soluble ◽  
Cell Turnover ◽  
Colon Cancer Cells ◽  
Human Colon Cancer ◽  
Physiological Level ◽  
Acid Supplementation

Folate, a water-soluble B vitamin, is a cofactor in one-carbon metabolism and is essential for DNA synthesis, amino acid interconversion, methylation and, consequently, normal cell growth. In animals with existing pre-neoplastic and neoplastic lesions, folic acid supplementation increases the tumour burden. To identify processes that are affected by increased folic acid levels, we compared HT29 human colon cancer cells exposed to a chronic supplemental (100 ng/ml) level of folic acid to cells exposed to a normal (10 ng/ml) level of folic acid, in the presence of vitamin B12and other micronutrients involved in the folate–methionine cycle. In addition to higher intracellular folate levels, HT29 cells at 100 ng folic acid/ml displayed faster growth and higher metabolic activity. cDNA microarray analysis indicated an effect on cell turnover and Fe metabolism. We fully confirmed these effects at the physiological level. At 100 ng/ml, cell assays showed higher proliferation and apoptosis, while gene expression analysis and a lower E-cadherin protein expression indicated decreased differentiation. These results are in agreement with the promoting effect of folic acid supplementation on established colorectal neoplasms. The lower expression of genes related to Fe metabolism at 100 ng folic acid/ml was confirmed by lower intracellular Fe levels in the cells exposed to folic acid at 100 ng/ml. This suggests an effect of folate on Fe metabolism.

scholarly journals Benzylidene derivatives of andrographolide inhibit growth of breast and colon cancer cells in vitro by inducing G1 arrest and apoptosis

2008 ◽  
Vol 155 (5) ◽  
pp. 641-654 ◽  
Author(s):  
S R Jada ◽  
C Matthews ◽  
M S Saad ◽  
A S Hamzah ◽  
N H Lajis ◽  
...  
Keyword(s):  
Colon Cancer ◽  
Cancer Cells ◽  
G1 Arrest ◽  
Colon Cancer Cells ◽  
Derivatives Of

scholarly journals Effect of Free Radical Scavengers on the Invasive Capacity of Colon Cancer Cells

1992 ◽  
Vol 25 (9) ◽  
pp. 2448-2448
Author(s):  
Yasuyuki Nonaka ◽  
Hiromi Iwagaki ◽  
Nagahide Matsubara ◽  
Toshikazu Kimura ◽  
Sadanori Fuchimoto ◽  
...  
Keyword(s):  
Colon Cancer ◽  
Free Radical ◽  
Cancer Cells ◽  
Free Radical Scavengers ◽  
Colon Cancer Cells ◽  
Radical Scavengers ◽  
Invasive Capacity
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