scholarly journals Identification and comparative analysis of hepatitis C virus–host cell protein interactions

2013 ◽  
Vol 9 (12) ◽  
pp. 3199 ◽  
Author(s):  
Patrick T. Dolan ◽  
Chaoying Zhang ◽  
Sudip Khadka ◽  
Vaithilingaraja Arumugaswami ◽  
Abbey D. Vangeloff ◽  
...  
Keyword(s):  
Hepatitis C Virus ◽  
Comparative Analysis ◽  
Hepatitis C ◽  
Host Cell ◽  
Protein Interactions ◽  
Cell Protein ◽  
Host Cell Protein

scholarly journals Intrinsic disorder mediates hepatitis C virus core-host cell protein interactions

2014 ◽  
Vol 24 (2) ◽  
pp. 221-235 ◽  
Author(s):  
Patrick T. Dolan ◽  
Andrew P. Roth ◽  
Bin Xue ◽  
Ren Sun ◽  
A. Keith Dunker ◽  
...  
Keyword(s):  
Hepatitis C Virus ◽  
Hepatitis C ◽  
Host Cell ◽  
Protein Interactions ◽  
Intrinsic Disorder ◽  
Cell Protein ◽  
Host Cell Protein ◽  
Virus Core

scholarly journals Using S. cerevisiae as a Model System to Investigate V. cholerae VopX-Host Cell Protein Interactions and Phenotypes

Toxins ◽  
2015 ◽  
Vol 7 (10) ◽  
pp. 4099-4110 ◽  
Author(s):  
Christopher Seward ◽  
Alexander Manzella ◽  
Ashfaqul Alam ◽  
J. Butler ◽  
Michelle Dziejman
Keyword(s):  
Host Cell ◽  
Protein Interactions ◽  
Model System ◽  
Cell Protein ◽  
Host Cell Protein

scholarly journals Comparative analysis of ChAdOx1 nCoV-19 and Ad26.COV2.S SARS-CoV-2 vector vaccines

2021 ◽  
Author(s):  
Stephan Michalik ◽  
Florian Siegerist ◽  
Raghavendra Palankar ◽  
Kati Franzke ◽  
Maximilian Schindler ◽  
...  
Keyword(s):  
Comparative Analysis ◽  
Host Cell ◽  
Incidence Rate ◽  
Platelet Factor 4 ◽  
Cell Protein ◽  
Capillary Leakage ◽  
Host Cell Protein ◽  
Platelet Factor ◽  
Causative Factors ◽  
Adenoviral Proteins

Abstract Vector-based SARS-CoV-2 vaccines have been associated with vaccine-induced thrombosis with thrombocytopenia syndrome (VITT/TTS), but the causative factors are still unresolved. We comprehensively analyzed ChAdOx1 nCov-19 (AstraZeneca) and Ad26.COV2.S (Johnson & Johnson). ChAdOx1 nCoV-19 contains significant amounts of host cell protein impurities, including functionally active proteasomes, and adenoviral proteins. In Ad26.COV2.S much less impurities were found. Platelet-factor 4 (PF4) formed complexes with ChAdOx1 nCoV-19 constituents, but not with purified virions from ChAdOx1 nCoV-19 or with Ad26.COV2.S. Vascular hyperpermeability was induced by ChAdOx nCoV-19 but not by Ad26.COV2.S.These differences in impurities together with EDTA-induced capillary leakage might contribute to the higher incidence rate of VITT associated with ChAdOx1 nCoV-19 compared to Ad26.COV2.S.

Studying host cell protein interactions with monoclonal antibodies using high throughput protein A chromatography

2012 ◽  
Vol 7 (10) ◽  
pp. 1233-1241 ◽  
Author(s):  
Vikram N. Sisodiya ◽  
Joshua Lequieu ◽  
Maricel Rodriguez ◽  
Paul McDonald ◽  
Kathlyn P. Lazzareschi
Keyword(s):  
Monoclonal Antibodies ◽  
Host Cell ◽  
High Throughput ◽  
Protein Interactions ◽  
Protein A ◽  
Cell Protein ◽  
Host Cell Protein

scholarly journals The Campylobacter jejuni CiaD effector co-opts the host cell protein IQGAP1 to promote cell entry

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Nicholas M. Negretti ◽  
Christopher R. Gourley ◽  
Prabhat K. Talukdar ◽  
Geremy Clair ◽  
Courtney M. Klappenbach ◽  
...  
Keyword(s):  
Host Cell ◽  
Campylobacter Jejuni ◽  
Foodborne Pathogen ◽  
Small Gtpase ◽  
Host Cells ◽  
Cell Protein ◽  
Host Cell Protein ◽  
Cell Binding ◽  
Actin Reorganization ◽  
Cell Cytosol

AbstractCampylobacter jejuni is a foodborne pathogen that binds to and invades the epithelial cells lining the human intestinal tract. Maximal invasion of host cells by C. jejuni requires cell binding as well as delivery of the Cia proteins (Campylobacter invasion antigens) to the host cell cytosol via the flagellum. Here, we show that CiaD binds to the host cell protein IQGAP1 (a Ras GTPase-activating-like protein), thus displacing RacGAP1 from the IQGAP1 complex. This, in turn, leads to the unconstrained activity of the small GTPase Rac1, which is known to have roles in actin reorganization and internalization of C. jejuni. Our results represent the identification of a host cell protein targeted by a flagellar secreted effector protein and demonstrate that C. jejuni-stimulated Rac signaling is dependent on IQGAP1.

Evaluating Immunogenicity Risk Due to Host Cell Protein Impurities in Antibody-Based Biotherapeutics

2016 ◽  
Vol 18 (6) ◽  
pp. 1439-1452 ◽  
Author(s):  
Vibha Jawa ◽  
Marisa K. Joubert ◽  
Qingchun Zhang ◽  
Meghana Deshpande ◽  
Suminda Hapuarachchi ◽  
...  
Keyword(s):  
Host Cell ◽  
Cell Protein ◽  
Host Cell Protein

scholarly journals Host cell protein platform assay development for therapeutic mAb bioprocessing using mammalian cells

2015 ◽  
Vol 9 (S9) ◽  
Author(s):  
Nadine Kochanowski ◽  
Gaetan Siriez ◽  
Larissa Mukankurayija ◽  
Aurélie Delangle ◽  
Alex Murray-Smith ◽  
...  
Keyword(s):  
Host Cell ◽  
Mammalian Cells ◽  
Assay Development ◽  
Cell Protein ◽  
Host Cell Protein

Hepatitis C virus NS2 protease inhibits host cell antiviral response by inhibiting IKKε and TBK1 functions

2012 ◽  
Vol 85 (1) ◽  
pp. 71-82 ◽  
Author(s):  
Pasi Kaukinen ◽  
Maarit Sillanpää ◽  
Laura Nousiainen ◽  
Krister Melén ◽  
Ilkka Julkunen
Keyword(s):  
Hepatitis C Virus ◽  
Hepatitis C ◽  
Host Cell ◽  
Antiviral Response
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