Ion-pairing chromatography on a porous graphitic carbon column coupled with time-of-flight mass spectrometry for targeted and untargeted profiling of amino acid biomarkers involved in Candida albicans biofilm formation

2014 ◽  
Vol 10 (1) ◽  
pp. 74-85 ◽  
Author(s):  
Xiaofei Chen ◽  
Haitang Wu ◽  
Yan Cao ◽  
Xiangwen Yao ◽  
Liang Zhao ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Candida Albicans ◽  
Amino Acid ◽  
Biofilm Formation ◽  
Time Of Flight ◽  
Ion Pairing ◽  
Porous Graphitic Carbon ◽  
Graphitic Carbon ◽  
Flight Mass Spectrometry ◽  
Untargeted Profiling

scholarly journals Determination of the Complete Amino Acid Sequence for the Coat Protein of Brome Mosaic Virus by Time-of-Flight Mass Spectrometry

2001 ◽  
Vol 276 (23) ◽  
pp. 20039-20047 ◽  
Author(s):  
Yi-Min She ◽  
Steve Haber ◽  
Dallas L. Seifers ◽  
Alexander Loboda ◽  
Igor Chernushevich ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Amino Acid ◽  
Coat Protein ◽  
Amino Acid Sequence ◽  
Mosaic Virus ◽  
Time Of Flight ◽  
Brome Mosaic Virus ◽  
Complete Amino Acid Sequence ◽  
Flight Mass Spectrometry

scholarly journals Metabolic Analysis of the Development of the Plant-Parasitic Cyst Nematodes Heterodera schachtii and Heterodera trifolii by Capillary Electrophoresis Time-of-Flight Mass Spectrometry

2021 ◽  
Vol 22 (19) ◽  
pp. 10488
Author(s):  
Awraris Derbie Assefa ◽  
Seong-Hoon Kim ◽  
Vimalraj Mani ◽  
Hyoung-Rai Ko ◽  
Bum-Soo Hahn
Keyword(s):  
Mass Spectrometry ◽  
Capillary Electrophoresis ◽  
Amino Acid ◽  
Amino Acid Metabolism ◽  
Acid Metabolism ◽  
Time Of Flight ◽  
Heterodera Schachtii ◽  
Economic Losses ◽  
Cyst Nematodes ◽  
Flight Mass Spectrometry

The cyst nematodes Heterodera schachtii and Heterodera trifolii, whose major hosts are sugar beet and clover, respectively, damage a broad range of plants, resulting in significant economic losses. Nematodes synthesize metabolites for organismal development and social communication. We performed metabolic profiling of H. schachtii and H. trifolii in the egg, juvenile 2 (J2), and female stages. In all, 392 peaks were analyzed by capillary electrophoresis time-of-flight mass spectrometry, which revealed a lot of similarities among metabolomes. Aromatic amino acid metabolism, carbohydrate metabolism, choline metabolism, methionine salvage pathway, glutamate metabolism, urea cycle, glycolysis, gluconeogenesis, coenzyme metabolism, purine metabolism, pyrimidine metabolism, and tricarboxylic acid (TCA) cycle for energy conversion (β-oxidation and branched-chain amino acid metabolism) energy storage were involved in all stages studied. The egg and female stages synthesized higher levels of metabolites compared to the J2 stage. The key metabolites detected were glycerol, guanosine, hydroxyproline, citric acid, phosphorylcholine, and the essential amino acids Phe, Leu, Ser, and Val. Metabolites, such as hydroxyproline, acetylcholine, serotonin, glutathione, and glutathione disulfide, which are associated with growth and reproduction, mobility, and neurotransmission, predominated in the J2 stage. Other metabolites, such as SAM, 3PSer, 3-ureidopropionic acid, CTP, UDP, UTP, 3-hydroxy-3-methylglutaric acid, 2-amino-2-(hydroxymethyl-1,3-propanediol, 2-hydroxy-4-methylvaleric acid, Gly Asp, glucuronic acid-3 + galacturonic acid-3 Ser-Glu, citrulline, and γ-Glu-Asn, were highly detected in the egg stage. Meanwhile, nicotinamide, 3-PG, F6P, Cys, ADP-Ribose, Ru5P, S7P, IMP, DAP, diethanolamine, p-Hydroxybenzoic acid, and γ-Glu-Arg_divalent were unique to the J2 stage. Formiminoglutamic acid, nicotinaminde riboside + XC0089, putrescine, thiamine 2,3-dihydroxybenzoic acid, 3-methyladenine, caffeic acid, ferulic acid, m-hydrobenzoic acid, o- and p-coumaric acid, and shikimic acid were specific to the female stage. Overall, highly similar identities and quantities of metabolites between the corresponding stages of the two species of nematode were observed. Our results will be a valuable resource for further studies of physiological changes related to the development of nematodes and nematode–plant interactions.

scholarly journals Analyzing the Metabolomic Profile of Yellowtail (Seriola quinquerdiata) by Capillary Electrophoresis–Time of Flight Mass Spectrometry to Determine Geographical Origin

Metabolites ◽  
2021 ◽  
Vol 11 (11) ◽  
pp. 793
Author(s):  
Jiyoung Shin ◽  
Junho Yang ◽  
Eunji Cha ◽  
Hyunsuk Kim ◽  
Yoonhyeung Lee ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Amino Acids ◽  
Capillary Electrophoresis ◽  
Amino Acid ◽  
Geographical Origin ◽  
Feeding Habits ◽  
Time Of Flight ◽  
Fasting Period ◽  
Flight Mass Spectrometry ◽  
Amino Acid Contents

Country-of-origin violations have occurred in which some merchants have fraudulently sold cheap Japanese yellowtail (Seriola quinqueradiata) by presenting them as domestic Korean products. There are many methods for determining the origins of marine organisms, such as molecular genetic methods and isotope analysis. However, this study aimed to develop a method for determining the origins of aquatic products using metabolite analysis technology. Ten yellowtail each from Korea and Japan were analyzed by capillary electrophoresis–time of flight/mass spectrometry (CETOF/MS). Hierarchical cluster analysis (HCA) and principal component analysis (PCA) results showed highly differing aspects between the Korean and Japanese samples. In the tricarboxylic acid (TCA) cycle, citric, malic, oxaloglutaric, and fumaric acids exhibited significant differences between Korean and Japanese yellowtail. Sixteen of the twenty essential amino acids analyzed as metabolites also differed significantly. All amino acids were involved in protein digestion, absorption, and metabolism. All 16 amino acid contents were higher in Japanese yellowtail than in Korean yellowtail, except for glutamine. The fasting period was found to be the biggest factor contributing to the difference in amino acid contents, in addition to environmental factors (including feeding habits). These significant differences indicated that metabolomics could be used to determine geographical origin.

scholarly journals Amino acid analyses of Antarctic CM2 meteorites using liquid chromatography-time of flight-mass spectrometry

2006 ◽  
Vol 41 (6) ◽  
pp. 889-902 ◽  
Author(s):  
Daniel P. Glavin ◽  
Jason P. Dworkin ◽  
Andrew Aubrey ◽  
Oliver Botta ◽  
James H. Doty ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Liquid Chromatography ◽  
Amino Acid ◽  
Time Of Flight ◽  
Flight Mass Spectrometry

scholarly journals Effect of different slaughtering methods on metabolites of broiler chickens using Ultra High-Performance Liquid Chromatography-Time of Flight-Mass Spectrometry (UHPLC-TOF-MS)

Food Research ◽  
2020 ◽  
Vol 4 (S1) ◽  
pp. 133-138 ◽  
Author(s):  
N.S.M. Ali ◽  
A.R. Zabidi ◽  
M.N.A. Manap ◽  
S.M.S.N.S. Zahari ◽  
N. Yahaya
Keyword(s):  
Mass Spectrometry ◽  
Liquid Chromatography ◽  
Amino Acid ◽  
Broiler Chickens ◽  
High Performance ◽  
Time Of Flight ◽  
Flight Mass Spectrometry ◽  
Islamic Tradition ◽  
Scientific Facts ◽  
Tof Ms

Islamic study defined Halal meat as a “thoyyiban” (clean) food source. Halal meat is produced by following slaughtering procedure as determined by the Islamic jurisprudence. Slaughtering methods have gained a worldwide discussion as animal welfare becomes a concern. However, there is lacking of scientific facts to prove which slaughtering methods produce better physiological effects on animals from metabolomics view. Therefore, metabolomics approach by Liquid Chromatography-Time of Flight-Mass Spectrometry (LC-TOF-MS) was used in this study to understand how the metabolites in poultry change when subjected to different slaughtering processes. The broiler chickens were subjected to Halal (Islamic tradition) and non-Halal slaughtering method (neck poking) where pectoral major muscle tissues from the slaughtered meat were selected for UHPLC-TOF-MS analysis. Metabolome data highlighted multiple pathways affected by slaughtering methods including glucose, amino acid, inosine, hypoxanthine and arginine. Higher utilization of energy in non-Halal slaughtering process was observed as indicated by the increase of gluconeogenesis and amino acid breakdown. The result from this study indicated that the method of slaughter affects the metabolites profile of poultry.

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