scholarly journals Three-dimensional co-cultures of human endothelial cells and embryonic stem cell-derived pericytes inside a microfluidic device

Lab on a Chip ◽  
2013 ◽  
Vol 13 (18) ◽  
pp. 3562 ◽  
Author(s):  
Andries D. van der Meer ◽  
Valeria V. Orlova ◽  
Peter ten Dijke ◽  
Albert van den Berg ◽  
Christine L. Mummery
Keyword(s):  
Endothelial Cells ◽  
Stem Cell ◽  
Embryonic Stem Cell ◽  
Microfluidic Device ◽  
Three Dimensional ◽  
Embryonic Stem ◽  
Human Endothelial Cells

scholarly journals Hydrogel platform for in vitro three-dimensional assembly of human stem cell-derived β cells and endothelial cells

2019 ◽  
Author(s):  
Punn Augsornworawat ◽  
Leonardo Velazco-Cruz ◽  
Jiwon Song ◽  
Jeffrey R. Millman
Keyword(s):  
Endothelial Cells ◽  
Stem Cell ◽  
Embryonic Stem Cell ◽  
Human Embryonic Stem Cell ◽  
Three Dimensional ◽  
Embryonic Stem ◽  
Cell Types ◽  
Diabetic Patients ◽  
Β Cells

AbstractDifferentiation of stem cells into functional replacement cells and tissues is a major goal of the regenerative medicine field. However, one limitation has been organization of differentiated cells into multi-cellular, three-dimensional assemblies. The islets of Langerhans contain many endocrine and non-endocrine cell types, such as insulin-producing β cells and endothelial cells. Transplantation of exogenous islets into diabetic patients can serve as a cell replacement therapy, replacing the need for patients to inject themselves with insulin, but the number of available islets from cadaveric donors is low. We have developed a strategy of assembling human embryonic stem cell-derived β cells with endothelial cells into three-dimensional aggregates on a hydrogel. The resulting islet organoids express β cell markers and are functional, capable of undergoing glucose-stimulated insulin secretion. These results provide a platform for evaluating the effects of the islet tissue microenvironment on human embryonic stem cell-derived β cells and other islet endocrine cells to develop tissue engineered islets.

Use of Embryonic Stem Cell-Derived Endothelial Cells as a Cell Source to Generate Vessel Structuresin Vitro

2005 ◽  
Vol 11 (3-4) ◽  
pp. 497-505 ◽  
Author(s):  
Kara E. McCloskey ◽  
Meghan E. Gilroy ◽  
Robert M. Nerem
Keyword(s):  
Endothelial Cells ◽  
Stem Cell ◽  
Embryonic Stem Cell ◽  
Embryonic Stem ◽  
Cell Source ◽  
A Cell

Tie-1-directed expression of Cre recombinase in endothelial cells of embryoid bodies and transgenic mice

2001 ◽  
Vol 114 (4) ◽  
pp. 671-676 ◽  
Author(s):  
E. Gustafsson ◽  
C. Brakebusch ◽  
K. Hietanen ◽  
R. Fassler
Keyword(s):  
Endothelial Cells ◽  
Stem Cell ◽  
Embryonic Stem Cell ◽  
Embryonic Stem ◽  
Cre Recombinase ◽  
Embryoid Bodies ◽  
Lymphoid Cells ◽  
Adult Brain ◽  
Specific Gene

Tissue-specific gene inactivation using the Cre-loxP system has become an important tool to unravel functions of genes when the conventional null mutation is lethal. We report here the generation of a transgenic mouse line expressing Cre recombinase in endothelial cells. In order to avoid the production and screening of multiple transgenic lines we used embryonic stem cell and embryoid body technology to identify recombinant embryonic stem cell clones with high, endothelial-specific Cre activity. One embryonic stem cell clone that showed high Cre activity in endothelial cells was used to generate germline chimeras. The in vivo efficiency and specificity of the transgenic Cre was analysed by intercrossing the tie-1-Cre line with the ROSA26R reporter mice. At initial stages of vascular formation (E8-9), LacZ staining was detected in almost all cells of the forming vasculature. Between E10 and birth, LacZ activity was detected in most endothelial cells within the embryo and of extra-embryonic tissues such as yolk sac and chorioallantoic placenta. Ectopic expression of Cre was observed in approximately 12–20% of the adult erythroid, myeloid and lymphoid cells and in subregions of the adult brain. These results show that the tie-1-Cre transgenic strain can efficiently direct deletion of floxed genes in endothelial cells in vivo.

scholarly journals nAChRs Mediate Human Embryonic Stem Cell-Derived Endothelial Cells: Proliferation, Apoptosis, and Angiogenesis

PLoS ONE ◽  
2009 ◽  
Vol 4 (9) ◽  
pp. e7040 ◽  
Author(s):  
Jin Yu ◽  
Ngan F. Huang ◽  
Kitchener D. Wilson ◽  
Jeffrey B. Velotta ◽  
Mei Huang ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Stem Cell ◽  
Embryonic Stem Cell ◽  
Human Embryonic Stem Cell ◽  
Embryonic Stem

scholarly journals Bioassembly of three-dimensional embryonic stem cell-scaffold complexes using compressed gases

2009 ◽  
Vol 25 (2) ◽  
pp. 535-542 ◽  
Author(s):  
Yubing Xie ◽  
Yong Yang ◽  
Xihai Kang ◽  
Ruth Li ◽  
Leonithas I. Volakis ◽  
...  
Keyword(s):  
Stem Cell ◽  
Embryonic Stem Cell ◽  
Three Dimensional ◽  
Embryonic Stem ◽  
Cell Scaffold
Sign in / Sign up

Export Citation Format

Share Document