A high-throughput method for the determination of Os concentrations and isotope ratio measurements in small-size biological samples

2013 ◽  
Vol 28 (10) ◽  
pp. 1591 ◽  
Author(s):  
Nicola Pallavicini ◽  
Frauke Ecke ◽  
Emma Engström ◽  
Douglas C. Baxter ◽  
Ilia Rodushkin
Keyword(s):  
High Throughput ◽  
Biological Samples ◽  
Isotope Ratio ◽  
High Throughput Method ◽  
Isotope Ratio Measurements

High-throughput method for the determination of residues of β-lactam antibiotics in bovine milk by LC-MS/MS

2015 ◽  
pp. 1-10 ◽  
Author(s):  
Louise Jank ◽  
Magda Targa Martins ◽  
Juliana Bazzan Arsand ◽  
Rodrigo Barcellos Hoff ◽  
Fabiano Barreto ◽  
...  
Keyword(s):  
High Throughput ◽  
Bovine Milk ◽  
High Throughput Method

scholarly journals Method for the determination of concentration and stable carbon isotope ratios of atmospheric phenols

2013 ◽  
Vol 6 (11) ◽  
pp. 2965-2974 ◽  
Author(s):  
M. Saccon ◽  
R. Busca ◽  
C. Facca ◽  
L. Huang ◽  
S. Irei ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Isotopic Composition ◽  
Isotope Ratio ◽  
Extraction Procedure ◽  
Carbon Isotopic Composition ◽  
Stable Carbon ◽  
Concentration Measurements ◽  
Isotope Ratio Measurements

Abstract. A method for the determination of the stable carbon isotopic composition of atmospheric nitrophenols in the gas and particulate phases is presented. It has been proposed to use the combination of concentration and isotope ratio measurements of precursor and product to test the applicability of results of laboratory studies to the atmosphere. Nitrophenols are suspected to be secondary products formed specifically from the photooxidation of volatile organic compounds. XAD-4TM resin was used as an adsorbent on quartz filters to sample ambient phenols using conventional high volume air samplers at York University in Toronto, Canada. Filters were extracted in acetonitrile, with a HPLC (high-performance liquid chromatography) clean-up step and a solid phase extraction step prior to derivatization with BSTFA (bis(trimethylsilyl) trifluoroacetamide). Concentration measurements were done with gas chromatography–mass spectrometry and gas chromatography–isotope ratio mass spectrometry was used for isotope ratio analysis. The technique presented allows for atmospheric compound-specific isotopic composition measurements for five semi-volatile phenols with an estimated accuracy of 0.3–0.5‰ at atmospheric concentrations exceeding 0.1 ng m−3 while the detection limits for concentration measurements are in the pg m−3 range. Isotopic fractionation throughout the entire extraction procedure and analysis was proven to be below the precision of the isotope ratio measurements. The method was tested by conducting ambient measurements from September to December 2011.

High throughput method for the determination of organochlorine pesticides and polychlorinated biphenyls in human serum

2007 ◽  
Vol 852 (1-2) ◽  
pp. 15-21 ◽  
Author(s):  
Fernando Goñi ◽  
Raul López ◽  
Arsenio Etxeandia ◽  
Esmeralda Millán ◽  
Pilar Amiano
Keyword(s):  
Polychlorinated Biphenyls ◽  
Human Serum ◽  
Organochlorine Pesticides ◽  
High Throughput ◽  
High Throughput Method

Picomol Assay of Nitric Oxide Screening in Biological Samples by Derivatization Combined with High-Throughput Microplate Format

2013 ◽  
Vol 423-426 ◽  
pp. 1786-1789
Author(s):  
Xiao Ling Zhang ◽  
Qiao Yang
Keyword(s):  
Nitric Oxide ◽  
Detection Limit ◽  
High Throughput ◽  
Biological Samples ◽  
Biological Sample ◽  
Small Volume ◽  
Screening Method ◽  
Broad Variety ◽  
Sensitive Determination

Nitric oxide (NO) is a critical intra-and extra-cellular signaling molecule that mediates a broad variety of physiologic and pathophysiologic events, and has prompted rapid growth of investigations in investigating its physiology and generation mechanism of corresponding diseases. In this paper, a picomol assay of NO screening method using fluorescent derivatization combined with high-throughout microplate format has been developed and successfully applied to the determination of NO in biological samples. The satisfied detection limit and recovery of the proposed method demonstrates that it can be competent for the sensitive determination of NO in extremely small volume of biological sample.

scholarly journals A high-throughput colourimetric method for the determination of pH in crystallization screens

2014 ◽  
Vol 70 (9) ◽  
pp. 2367-2375 ◽  
Author(s):  
Jobie Kirkwood ◽  
Julie Wilson ◽  
Simon O'Keefe ◽  
David Hargreaves
Keyword(s):  
High Throughput ◽  
Careful Control ◽  
High Throughput Method ◽  
Crystallization Solution

The crystallization of proteins is dependent on the careful control of numerous parameters, one of these being pH. The pH of crystallization is generally reported as that of the buffer; however, the true pH has been found to be as many as four pH units away. Measurement of pH with a meter is time-consuming and requires the reformatting of the crystallization solution. To overcome this, a high-throughput method for pH determination of buffered solutions has been developed with results comparable to those of a pH meter.

Sign in / Sign up

Export Citation Format

Share Document