Sample introduction of single selenized yeast cells (Saccharomyces cerevisiae) by micro droplet generation into an ICP-sector field mass spectrometer for label-free detection of trace elements

2013 ◽  
Vol 28 (5) ◽  
pp. 637 ◽  
Author(s):  
Kaori Shigeta ◽  
Gunda Koellensperger ◽  
Evelyn Rampler ◽  
Heike Traub ◽  
Lothar Rottmann ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Trace Elements ◽  
Mass Spectrometer ◽  
Droplet Generation ◽  
Sample Introduction ◽  
Yeast Cells ◽  
Label Free ◽  
Field Mass ◽  
Label Free Detection ◽  
Selenized Yeast

scholarly journals Source and origin of atmospheric trace elements entrapped in winter snow of the Italian Eastern Alps

2006 ◽  
Vol 6 (5) ◽  
pp. 8781-8815 ◽  
Author(s):  
P. Gabrielli ◽  
G. Cozzi ◽  
S. Torcini ◽  
P. Cescon ◽  
C. Barbante
Keyword(s):  
Boundary Layer ◽  
Trace Elements ◽  
Mass Spectrometer ◽  
Inductively Coupled Plasma ◽  
Major Ions ◽  
Eastern Alps ◽  
Field Mass ◽  
Inductively Coupled ◽  
Winter Snow ◽  
Snow Samples

Abstract. Trace elements concentrations were determined in shallow snow samples from 21 sites in the Italian Eastern Alps in order to identify the sources of the contaminants present in the tropospheric winter boundary layer. The collection of superficial snow layers was carried out weekly at altitudes between 1000 and 3000 m next to meteorological stations, far away from villages, roads and ski slopes. Ultra clean procedures were adopted in order to avoid contamination of the snow during the different experimental phases. Trace elements (Ag, Ba, Bi, Cd, Co, Cr, Cu, Fe, Mo, Mn, Pb, Sb, Ti, U, V and Zn) were determined by Inductively Coupled Plasma Sector Field Mass Spectrometer (ICP-SFMS). Ancillary parameters such as major ions (SO42−, NO3−, Ca2+;, Mg2+, K

Functionalized Polymers as Receptors for Detection of Cells

2011 ◽  
Vol 64 (9) ◽  
pp. 1256 ◽  
Author(s):  
Miroslava Polreichova ◽  
Usman Latif ◽  
Franz L. Dickert
Keyword(s):  
Soft Lithography ◽  
Quartz Crystal ◽  
Coli Strain ◽  
Yeast Cells ◽  
Label Free ◽  
Sensitive Material ◽  
E Coli ◽  
Label Free Detection ◽  
Quartz Crystal Microbalances ◽  
Cell Strains

Mass sensitive sensors were applied for fast and label-free detection of bio-analytes. Robust and miniaturized sensor devices were fabricated by combining bio-mimetic imprinted surfaces with quartz crystal microbalances for the analysis of yeast and bacteria cells. These sensors allow us to differentiate between different growing stages of yeast cells. Moreover, the viability of cells was detected by structuring quartz crystal microbalance electrodes like a grid. Artificial yeast cells were produced to pattern the recognition layer, giving reversible enrichment of the respective bio-analytes. This approach was followed to ensure the reproducibility of the identical sensitive material in each case, because the properties of each cell depend on its growth stage, which varies over time. The strategy was further applied to develop a sensitive system for Escherichia coli. Structuring of these materials by soft lithography allows differentiation between cell strains, e.g. E. coli (strain W & B) with a five-fold selectivity.

scholarly journals The Role of Surface Chemistry in the Efficacy of Protein and DNA Microarrays for Label-Free Detection: An Overview

Polymers ◽  
2021 ◽  
Vol 13 (7) ◽  
pp. 1026
Author(s):  
Elisa Chiodi ◽  
Allison M. Marn ◽  
Matthew T. Geib ◽  
M. Selim Ünlü
Keyword(s):  
Surface Chemistry ◽  
Surface Functionalization ◽  
Dna Microarrays ◽  
Label Free ◽  
Polymeric Coatings ◽  
Label Free Detection ◽  
Particle Imaging ◽  
Single Particle Imaging ◽  
The Impact

The importance of microarrays in diagnostics and medicine has drastically increased in the last few years. Nevertheless, the efficiency of a microarray-based assay intrinsically depends on the density and functionality of the biorecognition elements immobilized onto each sensor spot. Recently, researchers have put effort into developing new functionalization strategies and technologies which provide efficient immobilization and stability of any sort of molecule. Here, we present an overview of the most widely used methods of surface functionalization of microarray substrates, as well as the most recent advances in the field, and compare their performance in terms of optimal immobilization of the bioreceptor molecules. We focus on label-free microarrays and, in particular, we aim to describe the impact of surface chemistry on two types of microarray-based sensors: microarrays for single particle imaging and for label-free measurements of binding kinetics. Both protein and DNA microarrays are taken into consideration, and the effect of different polymeric coatings on the molecules’ functionalities is critically analyzed.

scholarly journals Label-Free Electrochemical Sensor for Rapid Bacterial Pathogen Detection Using Vancomycin-Modified Highly Branched Polymers

Sensors ◽  
2021 ◽  
Vol 21 (5) ◽  
pp. 1872
Author(s):  
Holger Schulze ◽  
Harry Wilson ◽  
Ines Cara ◽  
Steven Carter ◽  
Edward N. Dyson ◽  
...  
Keyword(s):  
Pathogen Detection ◽  
Electrochemical Impedance ◽  
Point Of Care ◽  
Branched Polymers ◽  
Label Free ◽  
Conformation Change ◽  
Gram Positive ◽  
Gram Positive Bacteria ◽  
Staphylococcus Carnosus ◽  
Label Free Detection

Rapid point of care tests for bacterial infection diagnosis are of great importance to reduce the misuse of antibiotics and burden of antimicrobial resistance. Here, we have successfully combined a new class of non-biological binder molecules with electrochemical impedance spectroscopy (EIS)-based sensor detection for direct, label-free detection of Gram-positive bacteria making use of the specific coil-to-globule conformation change of the vancomycin-modified highly branched polymers immobilized on the surface of gold screen-printed electrodes upon binding to Gram-positive bacteria. Staphylococcus carnosus was detected after just 20 min incubation of the sample solution with the polymer-functionalized electrodes. The polymer conformation change was quantified with two simple 1 min EIS tests before and after incubation with the sample. Tests revealed a concentration dependent signal change within an OD600 range of Staphylococcus carnosus from 0.002 to 0.1 and a clear discrimination between Gram-positive Staphylococcus carnosus and Gram-negative Escherichia coli bacteria. This exhibits a clear advancement in terms of simplified test complexity compared to existing bacteria detection tests. In addition, the polymer-functionalized electrodes showed good storage and operational stability.

H2O2-sensitive quantum dots for the label-free detection of glucose

Talanta ◽  
2010 ◽  
Vol 82 (3) ◽  
pp. 997-1002 ◽  
Author(s):  
Mei Hu ◽  
Jing Tian ◽  
Hao-Ting Lu ◽  
Li-Xing Weng ◽  
Lian-Hui Wang
Keyword(s):  
Quantum Dots ◽  
Label Free ◽  
Label Free Detection
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