Chemopreventive effect of cactus (Opuntia humifusa) extracts: radical scavenging activity, pro-apoptosis, and anti-inflammatory effect in human colon (SW480) and breast cancer (MCF7) cells

2013 ◽  
Vol 4 (5) ◽  
pp. 681 ◽  
Author(s):  
Jinhee Kim ◽  
Kwang Hyun Jho ◽  
Young Hee Choi ◽  
Sang-Yong Nam
Keyword(s):  
Breast Cancer ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Human Colon ◽  
Scavenging Activity ◽  
Mcf7 Cells ◽  
Opuntia Humifusa ◽  
Chemopreventive Effect ◽  
Anti Inflammatory ◽  
Inflammatory Effect

scholarly journals Studies on In-vitro Anti-inflammatory and Antioxidant Potentials of Annona muricata Leaf Extracts

2020 ◽  
Vol 12 (2) ◽  
pp. 177-183
Author(s):  
Idorenyin Nwaehujor ◽  
Samuel Akande ◽  
Olubunmi Atolani ◽  
Gabriel Olatunji
Keyword(s):  
Hydrogen Peroxide ◽  
Cell Membrane ◽  
Antioxidant Activities ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Ethanol Extract ◽  
Scavenging Activity ◽  
Membrane Stabilization ◽  
Anti Inflammatory ◽  
Proteinase Inhibition

Inflammation has stimulated significant worldwide scientific interest because of its implication in many human diseases. Most inflammations are caused by reactive oxygen species or free radicals. Annona muricataleaf extracts were investigated for their in-vitroantioxidant and anti-inflammatory potentials. Annona muricataleavesweredried at room temperature, blended using a mill.and extracted with solvents of varying degree of polarities. The solventsused were hexane, ethyl acetate,and ethanol. After sequential extraction, the crude extracts were examined for their in-vitroanti-inflammatory activities on lipoxygenase inhibition, proteinase inhibition, albumin denaturation inhibition,and red blood cell membrane stabilization assays,while the antioxidant activities were examined using DPPH, ABTS and hydrogen peroxide assays. The results showed that the ethanol extract had significantlyhigher albumin denaturation inhibition activity at 500 μg/mL (p < 0.01). The activity of all the extracts on proteinase inhibition decreased with the increase in concentration of the extracts. Indomethacin (standard), ethanol extract,and ethyl acetate extract exhibited a dose dependent increase in lipoxygenase activity. The ethanol extract showed highred blood cell membrane stabilization activity at 500 μg/mL and the activity was comparable with that of the standard (diclofenac). Hydrogen peroxide scavenging activity of the extracts and standard (Vitamin C) were comparable at 20 –100 μg/mL. The ethanol extract showed significantly higher(p < 0.01) DPPH radical scavenging activity compared with other extracts. A similar trend was also observed for ABTS radical scavenging activity. Generally,the ethanol extract exhibited higher anti-inflammatory and antioxidant activities in most of the assays, this could be attributed to the polar compounds present in the extract.

scholarly journals Antioxidant and Potentially Anti-Inflammatory Activity of Anthocyanin Fractions from Pomace Obtained from Enzymatically Treated Raspberries

Antioxidants ◽  
2019 ◽  
Vol 8 (8) ◽  
pp. 299 ◽  
Author(s):  
Urszula Szymanowska ◽  
Barbara Baraniak
Keyword(s):  
Antioxidant Activity ◽  
Phenolic Compounds ◽  
Solid Phase ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Phase Extraction ◽  
Scavenging Activity ◽  
Hplc Technique ◽  
Anti Inflammatory

Raspberry pomace was obtained from raspberries subjected to enzymatic maceration using three commercial pectinolytic preparations (Pectinex Ultra SP-L, Pectinex Yield Mash, and Ultrazym AFP-L). Phenolic compounds were extracted and anthocyanin fractions were isolated using the SPE solid phase extraction technique. In the separated anthocyanin fractions, the content of individual compounds was determined by the HPLC technique and the antioxidant activity was assessed with four complementary methods (DPPH and ABTS radical scavenging activity, chelating Fe(II) power, and ferric reducing power). Potential anti-inflammatory properties were also identified as the ability to inhibit the activity of lipoxygenase and cyclooxygenase 2. For these enzymes, the type of inhibition was determined based on the Lineweaver–Burke plot.

scholarly journals Probiotic Cell-Free Supernatants Exhibited Anti-Inflammatory and Antioxidant Activity on Human Gut Epithelial Cells and Macrophages Stimulated with LPS

2018 ◽  
Vol 2018 ◽  
pp. 1-12 ◽  
Author(s):  
Stefania De Marco ◽  
Marzia Sichetti ◽  
Diana Muradyan ◽  
Miranda Piccioni ◽  
Giovanna Traina ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Lactobacillus Reuteri ◽  
Intestinal Inflammation ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Human Colon ◽  
Saccharomyces Boulardii ◽  
Inflammatory Activity ◽  
Industrialized Countries ◽  
Anti Inflammatory

The incidence of inflammatory bowel disease is increasing all over the world, especially in industrialized countries. The aim of the present work was to verify the anti-inflammatory activity of metabolites. In particular, cell-free supernatants ofLactobacillus acidophilus, Lactobacillus casei,Lactococcus lactis, Lactobacillus reuteri, andSaccharomyces boulardiihave been investigated. Metabolites produced by these probiotics were able to downregulate the expression of PGE-2 and IL-8 in human colon epithelial HT-29 cells. Moreover, probiotic supernatants can differently modulate IL-1β, IL-6, TNF-α, and IL-10 production by human macrophages, suggesting a peculiar anti-inflammatory activity. Furthermore, supernatants showed a significant dose-dependent radical scavenging activity. This study suggests one of the mechanisms by which probiotics exert their anti-inflammatory activity affecting directly the intestinal epithelial cells and the underlying macrophages. This study provides a further evidence to support the possible use of probiotic metabolites in preventing and downregulating intestinal inflammation as adjuvant in anti-inflammatory therapy.

scholarly journals Antioxidant, Anti-Inflammatory and Antibacterial Activities of the Seeds of A Sri Lankan Variety of Carica Papaya

2019 ◽  
Vol 12 (2) ◽  
pp. 539-547
Author(s):  
Akshani Anjula Wijesooriya ◽  
Srianthie A. Deraniyagala ◽  
Chamari M. Hettiarachchi
Keyword(s):  
Ascorbic Acid ◽  
Phenolic Content ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Total Phenolic ◽  
Sri Lankan ◽  
Scavenging Activity ◽  
Flavonoid Content ◽  
Anti Inflammatory

Plant based remedies are of much importance in healthcare due to low side effects. The effective constituents contained in seeds of a papaya have not been utilized efficiently in the production of medicines. The purpose of this study was driven towards determining the total phenolic content, total flavonoid content, antioxidant capacity, anti-inflammatory activity and antibacterial properties of the aqueous seeds extract of a Sri Lankan variety (Red Lady) of papaya (AESP). The AESP prepared according to the method of “Kasaya” in Ayurvedic medicine was used for the investigations according to standard procedures. Total phenolic content and the total flavonoid content of the AESP were 13.5±2.2 mg (pyrogallol equivalence)/g and 315.9±104.6 mg (quercetin equivalence)/g respectively. The 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity of AESP ranged from 12.4% to 81.2% for concentrations of 1.0-20.0 mg/ml; ascorbic acid gave 31.7% - 91.9% activity. The AESP displayed strong reducing power compared to ascorbic acid in the reducing power assay. The hydroxyl radical scavenging activity of the AESP ranged from 6.6% to 69.1% for concentrations ranging from 20.0-95.0 mg/ml; ascorbic acid activity was 37.7%-74.9%. The nitric oxide radical scavenging activity of AESP was 23.9%-62.7% for concentrations of 2.0-20.0 µg/ml; ascorbic acid gave 26.8%-63.5% activity. AESP concentrations ranging from 75.0-150.0 µg/ml exhibited human red blood cell (HRBC) membrane stabilization protection of 15.5%-22.7% compared to 50.8%-58.4% for aspirin. The AESP showed antibacterial activity against Bacillus subtilis and Staphylococcus aureus bacterial species. AESP possess promising antioxidant, anti-inflammatory and antibacterial activity. The aqueous decoction of the discarded seeds of papaya would facilitate remedies for many diseases in which radicals are implicated as well as assist against certain bacterial infections and also has an anti-inflammatory potential.

scholarly journals In Vitro Antioxidant, Antiinflammation, and Anticancer Activities and Anthraquinone Content from Rumex crispus Root Extract and Fractions

Antioxidants ◽  
2020 ◽  
Vol 9 (8) ◽  
pp. 726 ◽  
Author(s):  
Taekil Eom ◽  
Ekyune Kim ◽  
Ju-Sung Kim
Keyword(s):  
Free Radical ◽  
Ethyl Acetate ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Raw 264.7 Cells ◽  
Root Extract ◽  
Scavenging Activity ◽  
Rumex Crispus ◽  
Anti Inflammatory

Rumex crispus is a perennial plant that grows in humid environments across Korea. Its roots are used in traditional Korean medicine to treat several diseases, including diseases of the spleen and skin and several inflammatory pathologies. In this study, different solvent fractions (n-hexane, dichloromethane, ethyl acetate, n-butanol, and aqueous fractions) from an ethanol extract of R. crispus roots were evaluated for the presence and composition of anthraquinone compounds and antioxidants by checking for such things as free radical scavenging activity, and electron and proton atom donating ability. In addition, anti-inflammatory activity was measured by NO scavenging activity and inflammatory cytokine production; furthermore, anti-cancer activity was measured by apoptosis-inducing ability. Polyphenolic and flavonoid compounds were shown to be abundant in the dichloromethane and ethyl acetate fractions, which also exhibited strong antioxidant activity, including free radical scavenging and positive results in FRAP, TEAC, and ORAC assays. HPLC analysis revealed that the dichloromethane fractions had higher anthraquinone contents than the other fractions; the major anthraquinone compounds included chrysophanol, emodin, and physcione. In addition, results of the anti-inflammatory assays showed that the ethyl acetate fraction showed appreciable reductions in the levels of nitric oxide and inflammatory cytokines (TNF-α, IL-1β, and IL-6) in Raw 264.7 cells. Furthermore, the anthraquinone-rich dichloromethane fraction displayed the highest anticancer activity when evaluated in a human hepatoma cancer cell line (HepG2), in which it induced increased apoptosis mediated by p53 and caspase activation.

scholarly journals Studies on Persicae Semen. III. Oxygen Radical Scavenging Activity of PR-B, an Anti-inflammatory Protein of Persicae Semen

1985 ◽  
Vol 105 (9) ◽  
pp. 895-901 ◽  
Author(s):  
SHIGERU ARICHI ◽  
MICHINORI KUBO ◽  
TADATO TANI ◽  
HIDEO NAKAMURA ◽  
CHIEKO IMAZU ◽  
...  
Keyword(s):  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Oxygen Radical ◽  
Scavenging Activity ◽  
Inflammatory Protein ◽  
Persicae Semen ◽  
Anti Inflammatory

scholarly journals Antioxidant and Anti-Inflammatory Activity Determination of One Hundred Kinds of Pure Chemical Compounds Using Offline and Online Screening HPLC Assay

2015 ◽  
Vol 2015 ◽  
pp. 1-13 ◽  
Author(s):  
Kwang Jin Lee ◽  
You Chang Oh ◽  
Won Kyung Cho ◽  
Jin Yeul Ma
Keyword(s):  
Antioxidant Activity ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Chemical Compounds ◽  
Cell Counting ◽  
Raw 264.7 Cells ◽  
Scavenging Activity ◽  
Dpph Radical ◽  
Abts Assay ◽  
Anti Inflammatory

This study investigated the antioxidant activity of one hundred kinds of pure chemical compounds found within a number of natural substances and oriental medicinal herbs (OMH). Three different methods were used to evaluate the antioxidant activity of DPPH radical-scavenging activity, ABTS radical-scavenging activity, and online screening HPLC-ABTS assays. The results indicated that 17 compounds exhibited better inhibitory activity against ABTS radical than DPPH radical. The IC50rate of a more practical substance is determined, and the ABTS assay IC50values of gallic acid hydrate, (+)-catechin hydrate, caffeic acid, rutin hydrate, hyperoside, quercetin, and kaempferol compounds were 1.03 ± 0.25, 3.12 ± 0.51, 1.59 ± 0.06, 4.68 ± 1.24, 3.54 ± 0.39, 1.89 ± 0.33, and 3.70 ± 0.15 μg/mL, respectively. The ABTS assay is more sensitive to identifying the antioxidant activity since it has faster reaction kinetics and a heightened response to antioxidants. In addition, there was a very small margin of error between the results of the offline-ABTS assay and those of the online screening HPLC-ABTS assay. We also evaluated the effects of 17 compounds on the NO secretion in LPS-stimulated RAW 264.7 cells and also investigated the cytotoxicity of 17 compounds using a cell counting kit (CCK) in order to determine the optimal concentration that would provide an effective anti-inflammatory action with minimum toxicity. These results will be compiled into a database, and this method can be a powerful preselection tool for compounds intended to be studied for their potential bioactivity and antioxidant activity related to their radical-scavenging capacity.

Free Radical Scavenging Activity and Anti-Inflammatory Property of the Saponin from Seeds of Camellia oleifera Abel

2012 ◽  
Vol 550-553 ◽  
pp. 1262-1265 ◽  
Author(s):  
Yong Ye ◽  
Ya Guo ◽  
Yang Fang Wang
Keyword(s):  
Free Radical ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Oil Extraction ◽  
Free Radical Scavenging ◽  
Free Radical Scavenging Activity ◽  
Scavenging Activity ◽  
Camellia Oleifera ◽  
Paw Oedema ◽  
Anti Inflammatory

Seeds of Camellia oleifera are used for edible oil extraction, but the defatted seeds are always discarded. In this study, saponin was separated from the seeds after oil extraction by hexane and 70% aqueous methanol, purified by macro-reticular resin AB-8 and crystallized with 80% aqueous ethanol. Its structure was identified by MS, IR, 1H NMR and 13C NMR analysis. The DPPH radical scavenging activity was determined. Carrageenan-induced paw oedema in rats and Croton-oil induced ear inflammation in mice were tested for its anti-inflammatory ability. The results show the saponin has significant anti-inflammatory effects and free radical scavenging activity.

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