The influence of hard substratum reflection and calibration profiles on in situ fluorescence measurements of benthic microalgal biomass

2013 ◽  
Vol 15 (4) ◽  
pp. 783 ◽  
Author(s):  
Corina Carpentier ◽  
Anna Dahlhaus ◽  
Nick van de Giesen ◽  
Blahoslav Maršálek
Keyword(s):  
Hard Substratum ◽  
Microalgal Biomass ◽  
Fluorescence Measurements

scholarly journals Statistical approach for the retrieval of phytoplankton community structures from in situ fluorescence measurements

2016 ◽  
Vol 24 (21) ◽  
pp. 23635 ◽  
Author(s):  
Shengqiang Wang ◽  
Cong Xiao ◽  
Joji Ishizaka ◽  
Zhongfeng Qiu ◽  
Deyong Sun ◽  
...  
Keyword(s):  
Statistical Approach ◽  
Phytoplankton Community ◽  
Community Structures ◽  
Fluorescence Measurements

Surface layer variability in the Ross Sea, Antarctica as assessed by in situ fluorescence measurements

2011 ◽  
Vol 88 (1-4) ◽  
pp. 28-45 ◽  
Author(s):  
Walker O. Smith ◽  
Vernon Asper ◽  
Sasha Tozzi ◽  
Xiao Liu ◽  
Sharon E. Stammerjohn
Keyword(s):  
Surface Layer ◽  
Ross Sea ◽  
Fluorescence Measurements

scholarly journals Comparative Resin Kinetics Using in Situ Fluorescence Measurements

2002 ◽  
Vol 4 (5) ◽  
pp. 540-540
Author(s):  
Daniel P. Walsh ◽  
Christopher Pang ◽  
Puja B. Parikh ◽  
Young-Soo Kim ◽  
Young-Tae Chang
Keyword(s):  
Fluorescence Measurements

Improving the FAME Yield of in Situ Transesterification from Microalgal Biomass through Particle Size Reduction and Cosolvent Incorporation

2015 ◽  
Vol 29 (2) ◽  
pp. 823-832 ◽  
Author(s):  
Pamela Hidalgo ◽  
Gustavo Ciudad ◽  
Sigurd Schober ◽  
Martin Mittelbach ◽  
Rodrigo Navia
Keyword(s):  
Particle Size ◽  
Size Reduction ◽  
In Situ Transesterification ◽  
Particle Size Reduction ◽  
Microalgal Biomass

scholarly journals Environmental Forcing of Red Tides in the Southern Benguela

2014 ◽  
Vol 2014 ◽  
pp. 1-16 ◽  
Author(s):  
Mark R. Jury
Keyword(s):  
Climate Model ◽  
Late Summer ◽  
Temperature Inversion ◽  
Lower Atmosphere ◽  
Red Tides ◽  
Environmental Forcing ◽  
Fluorescence Measurements ◽  
Cape Peninsula ◽  
Dominant Feature

The Southern Benguela cape upwelling plumes have inshore wind shadows prone to red tides in late summer. Their intensity and coverage are estimated by satellite fluorescence measurements in the period 1997–2012 and qualified by in situ reports. High satellite fluorescence cases are identified at daily to seasonal time scales, and characteristics of the upper ocean and lower atmosphere are studied using third generation reanalyses. A dominant feature is easterly winds over the Cape Peninsula (34°S, 18°E) induced by a ridging anticyclone-coastal low weather pattern. Over Cape Columbine (33°S), there is a wind shadow with cyclonic wind and current shear. Composite atmospheric profiles reveal a 4°C temperature inversion near 500 m that traps a coastal wind jet >6 m/s below 200 m. The composite shelf oceanography shows a relic upwelling plume below 10 m overtopped by warmer water near the coast, providing the thermal stratification needed for biotic aggregation. Data from the IPSL5 coupled climate model over the period 1980–2080 indicates that environmental conditions favoring red tides may become more frequent.

Temporally and Spatially Resolved x-ray Fluorescence Measurements of in-situ Drug Concentration in Metered-Dose Inhaler Sprays

2015 ◽  
Vol 33 (4) ◽  
pp. 816-825 ◽  
Author(s):  
Daniel J. Duke ◽  
Alan L. Kastengren ◽  
Nicholas Mason-Smith ◽  
Yang Chen ◽  
Paul M. Young ◽  
...  
Keyword(s):  
Drug Concentration ◽  
Dose Inhaler ◽  
Metered Dose Inhaler ◽  
X Ray ◽  
Spatially Resolved ◽  
Fluorescence Measurements ◽  
Metered Dose

scholarly journals Automated Online Flow Cytometry Advances Microalgal Ecosystem Management as in situ, High-Temporal Resolution Monitoring Tool

2021 ◽  
Vol 9 ◽  
Author(s):  
Iris Haberkorn ◽  
Cosima L. Off ◽  
Michael D. Besmer ◽  
Leandro Buchmann ◽  
Alexander Mathys
Keyword(s):  
Flow Cytometry ◽  
Real Time ◽  
Temporal Resolution ◽  
High Temporal Resolution ◽  
Natural Ecosystems ◽  
Biotechnological Production ◽  
Microalgal Biomass ◽  
Bioprocess Optimization ◽  
Related Population

Microalgae are emerging as a next-generation biotechnological production system in the pharmaceutical, biofuel, and food domain. The economization of microalgal biorefineries remains a main target, where culture contamination and prokaryotic upsurge are main bottlenecks to impair culture stability, reproducibility, and consequently productivity. Automated online flow cytometry (FCM) is gaining momentum as bioprocess optimization tool, as it allows for spatial and temporal landscaping, real-time investigations of rapid microbial processes, and the assessment of intrinsic cell features. So far, automated online FCM has not been applied to microalgal ecosystems but poses a powerful technology for improving the feasibility of microalgal feedstock production through in situ, real-time, high-temporal resolution monitoring. The study lays the foundations for an application of automated online FCM implying far-reaching applications to impel and facilitate the implementation of innovations targeting at microalgal bioprocesses optimization. It shows that emissions collected on the FL1/FL3 fluorescent channels, harnessing nucleic acid staining and chlorophyll autofluorescence, enable a simultaneous assessment (quantitative and diversity-related) of prokaryotes and industrially relevant phototrophic Chlorella vulgaris in mixed ecosystems of different complexity over a broad concentration range (2.2–1,002.4 cells ⋅μL–1). Automated online FCM combined with data analysis relying on phenotypic fingerprinting poses a powerful tool for quantitative and diversity-related population dynamics monitoring. Quantitative data assessment showed that prokaryotic growth phases in engineered and natural ecosystems were characterized by different growth speeds and distinct peaks. Diversity-related population monitoring based on phenotypic fingerprinting indicated that prokaryotic upsurge in mixed cultures was governed by the dominance of single prokaryotic species. Automated online FCM is a powerful tool for microalgal bioprocess optimization owing to its adaptability to myriad phenotypic assays and its compatibility with various cultivation systems. This allows advancing bioprocesses associated with both microalgal biomass and compound production. Hence, automated online FCM poses a viable tool with applications across multiple domains within the biobased sector relying on single cell–based value chains.

scholarly journals Anions Enhance Rare Earth Adsorption at Negatively Charged Surfaces

2020 ◽  
Author(s):  
Srikanth Nayak ◽  
Kaitlin Lovering ◽  
Wei Bu ◽  
Ahmet Uysal
Keyword(s):  
Rare Earth ◽  
Electrostatic Interactions ◽  
Aqueous Interfaces ◽  
Charged Surfaces ◽  
Complex Processes ◽  
Specific Effects ◽  
Fluorescence Measurements ◽  
Adsorption Surface ◽  
Trivalent Rare Earth

Anions are expected to be repelled from negatively charged surfaces. At aqueous interfaces, however, ion-specific effects can dominate over direct electrostatic interactions. Using multiple <i>in situ</i> surface sensitive experimental techniques, we show that surface affinity of SCN<sup>-</sup> ions are so strong that they can adsorb at a negatively charged floating monolayer at the air/aqueous interface. This extreme example of ion-specific effects may be very important for understanding complex processes at aqueous interfaces, such as chemical separations of rare earth metals. Adsorbed SCN<sup>-</sup> ions at the floating monolayer increase the overall negative charge density, leading to enhanced trivalent rare earth adsorption. Surface sensitive X-ray fluorescence measurements show that the surface coverage of Lu<sup>3+</sup> ions can be triple of the apparent surface charge of the floating monolayer in the presence of SCN<sup>-</sup>. Comparison to NO<sub>3</sub><sup>-</sup> samples show that the effects are strongly dependent to the character of the anion, providing further evidence to ion-specific effects dominating over electrostatics.

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