Inhibition of quorum sensing and biofilm formation in Vibrio harveyi by 4-fluoro-DPD; a novel potent inhibitor of AI-2 signalling

2014 ◽  
Vol 50 (39) ◽  
pp. 5000-5002 ◽  
Author(s):  
Manikandan Kadirvel ◽  
Fariba Fanimarvasti ◽  
Sarah Forbes ◽  
Andrew McBain ◽  
John M. Gardiner ◽  
...  
Keyword(s):  
Quorum Sensing ◽  
Bacterial Growth ◽  
Biofilm Formation ◽  
Potent Inhibitor ◽  
Vibrio Harveyi

(±)4-Fluoro-DPD completely inhibited bioluminescence and bacterial growth of Vibrio harveyi BB170 strain by disruption of quorum sensing cascade.

scholarly journals Cannabigerol Prevents Quorum Sensing and Biofilm Formation of Vibrio harveyi

2020 ◽  
Vol 11 ◽  
Author(s):  
Muna Aqawi ◽  
Ruth Gallily ◽  
Ronit Vogt Sionov ◽  
Batya Zaks ◽  
Michael Friedman ◽  
...  
Keyword(s):  
Quorum Sensing ◽  
Biofilm Formation ◽  
Vibrio Harveyi

scholarly journals Modulation of Campylobacter jejuni Motility, Adhesion to Polystyrene Surfaces, and Invasion of INT407 Cells by Quorum-Sensing Inhibition

2020 ◽  
Vol 8 (1) ◽  
pp. 104 ◽  
Author(s):  
Katarina Šimunović ◽  
Dina Ramić ◽  
Changyun Xu ◽  
Sonja Smole Možina
Keyword(s):  
Quorum Sensing ◽  
Campylobacter Jejuni ◽  
Biofilm Formation ◽  
Vibrio Harveyi ◽  
Foodborne Pathogen ◽  
Wild Type ◽  
Natural Extracts ◽  
Ethanolic Extracts ◽  
Pure Compounds ◽  
Moderate Positive Correlation

Campylobacter jejuni is a major foodborne pathogen, and the LuxS-mediated quorum-sensing (QS) system influences its motility, biofilm formation, invasion, host colonization, and virulence. QS therefore represents a target for the control of C. jejuni. The aim of this study was to investigate the correlation of QS inhibition with changes in C. jejuni motility, adhesion to polystyrene surfaces, and adhesion to and invasion of INT407 cells. This was achieved by studying (i) the luxS-deficient mutant and (ii) treatment of C. jejuni with 20 natural extracts as six essential oils, 11 ethanolic extracts, and three pure compounds. Compared to the wild-type, the ΔluxS mutant showed decreased motility, adhesion to polystyrene surfaces, and invasion of INT407 cells. The anti-QS effects of the treatments (n = 15/20) were assayed using Vibrio harveyi BB170 bioluminescence. Moderate positive correlation was shown between C. jejuni QS reduction and reduced motility (τ = 0.492, p = 0.024), adhesion to polystyrene surfaces (τ = 0.419, p = 0.008), and invasion (r = 0.394, p = 0.068). The best overall effect was achieved with a Sedum rosea (roseroot) extract, with 96% QS reduction, a 1.41 log (96%) decrease in adhesion to polystyrene surfaces, and an 82% decrease in invasion. We show that natural extracts can reduce motility, adhesion to polystyrene surfaces, and invasion of INT407 cells by C. jejuni through modulation of the LuxS (QS) system.

Synergistic antibiofilm efficacy of undecanoic acid and auxins against quorum sensing mediated biofilm formation of luminescent Vibrio harveyi

Aquaculture ◽  
2019 ◽  
Vol 498 ◽  
pp. 162-170 ◽  
Author(s):  
Ramesh Salini ◽  
Sivasubramanian Santhakumari ◽  
Arumugam Veera Ravi ◽  
Shunmugiah Karutha Pandian
Keyword(s):  
Quorum Sensing ◽  
Biofilm Formation ◽  
Vibrio Harveyi ◽  
Undecanoic Acid

scholarly journals ANTI-QUORUM SENSING POTENTIAL OF LIMONIA ACIDISSIMA (L.) AGAINST VIBRIO HARVEYI KUMB-VA4

2018 ◽  
Vol 11 (3) ◽  
pp. 303
Author(s):  
Srinivasan P ◽  
Rajalakshmi M ◽  
Dinesh Kumar S
Keyword(s):  
Quorum Sensing ◽  
Ethyl Acetate ◽  
Molecular Characterization ◽  
Metabolic Activity ◽  
Biofilm Formation ◽  
Vibrio Harveyi ◽  
Dependent Manner ◽  
Biofilm Inhibition ◽  
Limonia Acidissima ◽  
Dose Dependent

 Objective: This study aims to investigate the quorum-sensing inhibition (QSI) potential of Limonia acidissima L. against the biofilm forming Vibrio harveyi isolated from freshwater fish.Methods: The present study evaluated the anti-QS activity of the L. acidissima methanol and ethyl acetate (LA-M and LA-EA) fruit extracts using Chromobacterium violaceum ATCC 12472 (wild) and C. violaceum CV026 (mutant) as biomonitor strains and biofilm formation using the crystal violet assay. Vibrio sp. were isolated from freshwater-cultured fishes and screened for biofilm formation property. Strong biofilm forming isolate were subjected to molecular characterization. Limonia fruit pulp was subjected to methanol and ethyl acetate extraction using cold percolation method and yield was calculated. In parallel to determining the QSI properties of the extract, minimum inhibitory concentration (MIC), biofilm inhibition concentration (BIC), antibiofilm properties, and metabolic activity of LA-M and LA-EA against the biofilm forming V. harveyi KUMB-VA4 was determined.Results: The results of the present study demonstrated that the overall yield of methanol and ethyl acetate extract was 12.84% and 9.3% (w/w), respectively. Strong biofilm forming Vibrio isolate KUMB-VA4 was obtained from infected freshwater fishes and was subjected to molecular characterization. MIC of LA-M was 1510 μg/ml and LA-EA was observed to be 3000 μg/ml against the test pathogen, respectively. Biofilm inhibition assay revealed a BIC of LA-M at 250 μg/ml and LA-EA at 500 μg/ml. Both the plant extracts significantly reduced the biofilm formation of V. harveyi KUMB-VA4 and the metabolic activity in a dose-dependent manner. Light microscopy and scanning electron microscopy revealed that LA-M and LA-EA significantly altered 68.6% and 54.5% of the biofilm architecture at BIC. The QSI assay revealed that LA-M effectively reduced the violacein production of the biomonitor strains at sub-BIC (100–500 μg/ml) to 80% than LA-EA (43%) in a strong dose-dependent fashion.Conclusions: The present study revealed the QSI property of Limonia acidissima against the biofilm forming V. harveyi isolated from infected fish.

scholarly journals Purification and Evaluation of N-benzyl Cinnamamide from Red Seaweed Gracilaria fisheri as an Inhibitor of Vibrio harveyi AI-2 Quorum Sensing

Marine Drugs ◽  
2020 ◽  
Vol 18 (2) ◽  
pp. 80 ◽  
Author(s):  
Kulwadee Karnjana ◽  
Saksit Nobsathian ◽  
Chumporn Soowannayan ◽  
Wei Zhao ◽  
Ya-Jie Tang ◽  
...  
Keyword(s):  
Quorum Sensing ◽  
Biofilm Formation ◽  
Inhibitory Concentration ◽  
Vibrio Harveyi ◽  
Ethanol Extract ◽  
Red Seaweed ◽  
In Vitro Activity ◽  
Activity Assay ◽  
Pure Compounds

Previously, we reported that the ethanol extract from red seaweed Gracilaria fisheri effectively decreased biofilm formation of Vibrio harveyi. In this study, the anti-biofilm active compounds in the ethanol extract were isolated and their structures identified. The anti-biofilm fractionation assay for minimum inhibitory concentration (MIC) produced two fractions which possessed maximal inhibitory activities toward the biofilm formation of V. harveyi strains 1114 and BAA 1116. Following chromatographic separation of the bioactive fractions, two pure compounds were isolated, and their structures were elucidated using FTIR, NMR, and HR-TOF-MS. The compounds were N-benzyl cinnamamide and α-resorcylic acid. The in vitro activity assay demonstrated that both compounds inhibited the biofilm formation of V. harveyi and possessed the anti-quorum sensing activity by interfering with the bioluminescence of the bacteria. However, the N-benzyl cinnamamide was more potent than α-resorcylic acid with a 10-fold lesser MIC. The present study reveals the beneficial property of the N-benzyl cinnamamide from the ethanol extract as a lead anti-microbial drug against V. harveyi.

In vitro antibiofilm efficacy of Piper betle against quorum sensing mediated biofilm formation of luminescent Vibrio harveyi

2017 ◽  
Vol 110 ◽  
pp. 232-239 ◽  
Author(s):  
Ramanathan Srinivasan ◽  
Sivasubramanian Santhakumari ◽  
Arumugam Veera Ravi
Keyword(s):  
Quorum Sensing ◽  
Biofilm Formation ◽  
Vibrio Harveyi ◽  
Piper Betle
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