Colorimetric assay of K-562 cells based on folic acid-conjugated porous bimetallic Pd@Au nanoparticles for point-of-care testing

2014 ◽  
Vol 50 (4) ◽  
pp. 475-477 ◽  
Author(s):  
Shenguang Ge ◽  
Fang Liu ◽  
Weiyan Liu ◽  
Mei Yan ◽  
Xianrang Song ◽  
...  
Keyword(s):  
Folic Acid ◽  
Au Nanoparticles ◽  
Colorimetric Assay ◽  
Point Of Care ◽  
Point Of Care Testing ◽  
K 562 Cells

scholarly journals A Smartphone Camera Colorimetric Assay of Acetylcholinesterase and Butyrylcholinesterase Activity

Sensors ◽  
2021 ◽  
Vol 21 (5) ◽  
pp. 1796
Author(s):  
Miroslav Pohanka ◽  
Jitka Zakova
Keyword(s):  
Limit Of Detection ◽  
Colorimetric Assay ◽  
Point Of Care ◽  
Specific Treatment ◽  
Point Of Care Testing ◽  
Analytical Instruments ◽  
Colorimetric Test ◽  
3D Printed ◽  
Butyrylcholinesterase Activity ◽  
Additional Education

Acetylcholinesterase (AChE) and butyrylcholinesterase (BChE) can serve as biochemical markers of various pathologies like liver disfunction and poisonings by nerve agents. Ellman’s assay is the standard spectrophotometric method to measure cholinesterase activity in clinical laboratories. The authors present a new colorimetric test to assess AChE and BChE activity in biological samples using chromogenic reagents, treated 3D-printed measuring pads and a smartphone camera as a signal detector. Multiwell pads treated with reagent substrates 2,6-dichlorophenolindophenyl acetate, indoxylacetate, ethoxyresorufin and methoxyresorufin were prepared and tested for AChE and BChE. In the experiments, 3D-printed pads containing indoxylacetate as a chromogenic substrate were optimal for analytical purposes. The best results were achieved using the red (R) channel, where the limit of detection was 4.05 µkat/mL for BChE and 4.38 µkat/mL for AChE using a 40 µL sample and a 60 min assay. The major advantage of this method is its overall simplicity, as samples are applied directly without any specific treatment or added reagents. The assay was also validated to the standard Ellman’s assay using human plasma samples. In conclusion, this smartphone camera-based colorimetric assay appears to have practical applicability and to be a suitable method for point-of-care testing because it does not require specific manipulation, additional education of staff or use of sophisticated analytical instruments.

Exploiting LBL-assembled Au nanoparticles to enhance Raman signals for point-of-care testing of osteoporosis with excreta sample

2017 ◽  
Vol 123 (2) ◽  
Author(s):  
Jian F. Sun ◽  
Xuan Liu ◽  
Zhi R. Guo ◽  
Jian Dong ◽  
Yawen Huang ◽  
...  
Keyword(s):  
Au Nanoparticles ◽  
Point Of Care ◽  
Point Of Care Testing

Electrochemical K-562 cells sensor based on origami paper device for point-of-care testing

Talanta ◽  
2015 ◽  
Vol 145 ◽  
pp. 12-19 ◽  
Author(s):  
Shenguang Ge ◽  
Lina Zhang ◽  
Yan Zhang ◽  
Haiyun Liu ◽  
Jiadong Huang ◽  
...  
Keyword(s):  
Point Of Care ◽  
Point Of Care Testing ◽  
Paper Device ◽  
K 562 Cells

Magnetic Digital Microfluidics for Point-of-Care Testing: Where Are We Now?

2020 ◽  
Vol 27 ◽  
Author(s):  
Yi Zhang
Keyword(s):  
System Integration ◽  
Diagnostic Tests ◽  
Point Of Care ◽  
Digital Microfluidics ◽  
Controlled Environment ◽  
Point Of Care Testing ◽  
Microfluidic Platforms ◽  
Interface System ◽  
Technical Issues ◽  
Sample System

: Point-of-care (POC) testing decentralizes the diagnostic tests to the sites near the patient. Many POC tests rely microfluidic platforms for sample-to-answer analysis. Compared to other microfluidic systems, magnetic digital microfluidics demonstrate compelling advantages for POC diagnostics. In this review, we have examined the capability of magnetic digital microfluidics-based POC diagnostic platforms. More importantly, we have categorized POC settings into three classes based on “where is the point”, “who to care” and “how to test”, and evaluated the suitability of magnetic digital microfluidics in various POC settings. Furthermore, we have addressed other technical issues associated with POC testing such as controlled environment, sample-system interface, system integration and information connectivity. We hope this review would provide a guideline for the future development of magnetic digital microfluidics-based platforms for POC testing.

Sign in / Sign up

Export Citation Format

Share Document