Translational repression using BIV Tat peptide–TAR RNA interaction in mammalian cells

2013 ◽  
Vol 49 (67) ◽  
pp. 7457 ◽  
Author(s):  
Chaitanya Sudrik ◽  
Manish Arha ◽  
Jicong Cao ◽  
David V. Schaffer ◽  
Ravi S. Kane
Keyword(s):  
Mammalian Cells ◽  
Translational Repression ◽  
Tat Peptide ◽  
Tar Rna ◽  
Rna Interaction

A New Strategy for Site-Specific Protein Modification:  Analysis of a Tat Peptide−TAR RNA Interaction

2001 ◽  
Vol 12 (2) ◽  
pp. 135-138 ◽  
Author(s):  
Natarajan Tamilarasu ◽  
Jing Zhang ◽  
Seongwoo Hwang ◽  
Tariq M. Rana
Keyword(s):  
Protein Modification ◽  
Specific Protein ◽  
Tat Peptide ◽  
Site Specific ◽  
New Strategy ◽  
Tar Rna ◽  
Rna Interaction

scholarly journals Failure of translational repression in the phage f2 op3 mutant is not due to an altered coat protein-RNA interaction.

1984 ◽  
Vol 259 (1) ◽  
pp. 20-22
Author(s):  
J Carey ◽  
V Cameron ◽  
M Krug ◽  
P L de Haseth ◽  
O C Uhlenbeck
Keyword(s):  
Coat Protein ◽  
Translational Repression ◽  
Rna Interaction

scholarly journals Overcoming the design, build, test bottleneck for synthesis of nonrepetitive protein-RNA cassettes

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Noa Katz ◽  
Eitamar Tripto ◽  
Naor Granik ◽  
Sarah Goldberg ◽  
Orna Atar ◽  
...  
Keyword(s):  
Binding Site ◽  
Binding Sites ◽  
Mammalian Cells ◽  
Coat Proteins ◽  
Structural Determinants ◽  
Design Build ◽  
Machine Learning Approach ◽  
Successful Design ◽  
Experimental Findings ◽  
Rna Interaction

AbstractWe apply an oligo-library and machine learning-approach to characterize the sequence and structural determinants of binding of the phage coat proteins (CPs) of bacteriophages MS2 (MCP), PP7 (PCP), and Qβ (QCP) to RNA. Using the oligo library, we generate thousands of candidate binding sites for each CP, and screen for binding using a high-throughput dose-response Sort-seq assay (iSort-seq). We then apply a neural network to expand this space of binding sites, which allowed us to identify the critical structural and sequence features for binding of each CP. To verify our model and experimental findings, we design several non-repetitive binding site cassettes and validate their functionality in mammalian cells. We find that the binding of each CP to RNA is characterized by a unique space of sequence and structural determinants, thus providing a more complete description of CP-RNA interaction as compared with previous low-throughput findings. Finally, based on the binding spaces we demonstrate a computational tool for the successful design and rapid synthesis of functional non-repetitive binding-site cassettes.

scholarly journals microRNA-Mediated Messenger RNA Deadenylation Contributes to Translational Repression in Mammalian Cells

PLoS ONE ◽  
2009 ◽  
Vol 4 (8) ◽  
pp. e6783 ◽  
Author(s):  
Traude H. Beilharz ◽  
David T. Humphreys ◽  
Jennifer L. Clancy ◽  
Rolf Thermann ◽  
David I. K. Martin ◽  
...  
Keyword(s):  
Messenger Rna ◽  
Mammalian Cells ◽  
Translational Repression

HIV-1 Tat Peptide Binding to TAR RNA by Electrospray Ionization Mass Spectrometry

1997 ◽  
Vol 69 (24) ◽  
pp. 5130-5135 ◽  
Author(s):  
Kristin A. Sannes-Lowery ◽  
Peifeng Hu ◽  
David P. Mack ◽  
Houng-Yau Mei ◽  
Joseph A. Loo
Keyword(s):  
Mass Spectrometry ◽  
Electrospray Ionization ◽  
Electrospray Ionization Mass Spectrometry ◽  
Peptide Binding ◽  
Ionization Mass Spectrometry ◽  
Ionization Mass ◽  
Tat Peptide ◽  
Tar Rna ◽  
Hiv 1

scholarly journals Polyamidoamine dendrimers inhibit binding of Tat peptide to TAR RNA

FEBS Letters ◽  
2004 ◽  
Vol 563 (1-3) ◽  
pp. 241-245 ◽  
Author(s):  
Hong Zhao ◽  
Jinru Li ◽  
Fu Xi ◽  
Long Jiang
Keyword(s):  
Tat Peptide ◽  
Polyamidoamine Dendrimers ◽  
Tar Rna
Sign in / Sign up

Export Citation Format

Share Document