A highly sensitive resonance Rayleigh scattering method to discriminate a parallel-stranded G-quadruplex from DNA with other topologies and structures

2013 ◽  
Vol 49 (55) ◽  
pp. 6209 ◽  
Author(s):  
Yan Shi ◽  
Hong Qun Luo ◽  
Nian Bing Li
Keyword(s):  
Rayleigh Scattering ◽  
Resonance Rayleigh Scattering ◽  
Scattering Method ◽  
Highly Sensitive ◽  
G Quadruplex

A Highly Sensitive Resonance Rayleigh Scattering Method for Platelet-Derived Growth Factor Using Aptamer-Nanogold Probe as Catalyst of the Cu2O Particle Reaction

2013 ◽  
Vol 680 ◽  
pp. 137-140
Author(s):  
Dong Mei Yao ◽  
Gui Qing Wen ◽  
Jian Niao Tian ◽  
Zhi Liang Jiang ◽  
Ai Hui Liang
Keyword(s):  
Gold Nanoparticles ◽  
Growth Factor ◽  
Rayleigh Scattering ◽  
Sodium Citrate ◽  
Reducing Agent ◽  
Platelet Derived Growth Factor ◽  
Resonance Rayleigh Scattering ◽  
Scattering Method ◽  
Highly Sensitive

Using sodium citrate as reducing agent, different sizes of gold nanoparticles(GN) were prepared, and were modified by platelet-derived growth factor (PDGF) aptamer to obtain stable aptamer-nanogold probes (Apt-GN). The probe specifically combined with PDGF-AA to form Apt-GN-PDGF-AA clusters that exhibited a resonance Rayleigh scattering (RRS) peak at 550 nm. The RRS intensity ΔI550nmwas linear to the PDGF-AA concentration in the range of 0.33-40 ng/ml. The probe has strong catalysis of the Fehling reagent-glucose Cu2O particle reaction that can be monitored by RRS technique at 610 nm, but the cluster is very weak. When PDGF-AA concentration increased, the Apt-GN decreased, and the RRS intensity at 610 nm decreased. The decreased RRS intensity ΔI610nmwas linear to PDGF-AA concentration in the range of 0.03-26.67 ng/mL. Accordingly, two new aptamer-nanogold RRS methods were established.

Sign in / Sign up

Export Citation Format

Share Document