POSS-containing red fluorescent nanoparticles for rapid detection of aqueous fluoride ions

2013 ◽  
Vol 49 (41) ◽  
pp. 4631 ◽  
Author(s):  
Fanfan Du ◽  
Yinyin Bao ◽  
Bin Liu ◽  
Jiao Tian ◽  
Qianbiao Li ◽  
...  
Keyword(s):  
Rapid Detection ◽  
Fluorescent Nanoparticles ◽  
Fluoride Ions

scholarly journals Novel methods for the rapid detection of trace tetracyclines based on the fluorescence behaviours of Maillard reaction fluorescent nanoparticles

RSC Advances ◽  
2020 ◽  
Vol 10 (71) ◽  
pp. 43256-43261
Author(s):  
Xue-jing Si ◽  
Hong-ling Wang ◽  
Tun-hua Wu ◽  
Ping Wang
Keyword(s):  
Food Safety ◽  
Maillard Reaction ◽  
Rapid Detection ◽  
Fluorescent Nanoparticles ◽  
Fluorescent Products ◽  
Novel Methods

The Maillard reaction and its fluorescent products have attracted widespread attention in the field of food safety and biology.

Polyhedral oligosilsesquioxane tethered perylene diimide for application in optical limiting and rapid detection of fluoride ions

2019 ◽  
Vol 55 (20) ◽  
pp. 3012-3014 ◽  
Author(s):  
Tian Gao ◽  
Wen-Fa Zhou ◽  
Yang Zhao ◽  
Lei Shen ◽  
Wen-Ying Chang ◽  
...  
Keyword(s):  
Rapid Detection ◽  
Optical Limiting ◽  
Perylene Diimide ◽  
Fluoride Ions ◽  
Polyhedral Oligosilsesquioxane

Polyhedral oligosilsesquioxane tethered perylene diimide was designed and synthesized for application in optical limiting and rapid detection of F−.

scholarly journals A coumarin-based fluorescent probe for specific and rapid detection of fluoride ions

2018 ◽  
Vol 20 (4) ◽  
pp. 1-5 ◽  
Author(s):  
Zhen-Hui Xin ◽  
Ya-Li Meng ◽  
Yan-Hua Wu ◽  
Jian Wang ◽  
Zhi-Ke Feng ◽  
...  
Keyword(s):  
Quantitative Analysis ◽  
Fluorescence Intensity ◽  
Rapid Detection ◽  
Fluorescence Probe ◽  
Fluoride Concentration ◽  
Practical Application ◽  
Important Condition ◽  
Fluoride Ions ◽  
Silyl Group ◽  
Low Toxicity

Abstract A simple and coumarin-based fluorescence probe has been designed and synthesized with silyl group as recognition group of fluoride ions (F−) in this study. The results showed that the fluorescence intensity of the probe displayed prominent enhancement with addition of F− at 445 nm with incubation of 1 min. There was an excellent linear relationship between fluorescence intensity and fluoride concentration from 0 to 30 μM (0~0.57 ppm), which offered the important condition for the quantitative analysis. In addition, the highly selective response to fluorion, the low detection limit with 28 nM (0.532 ppb), low toxicity and bioimaging afforded an advantage for practical application and detecting fluoride in biological systerms.

scholarly journals Fluorescent Nanoparticle-Based Indirect Immunofluorescence Microscopy for Detection ofMycobacterium tuberculosis

2007 ◽  
Vol 2007 ◽  
pp. 1-9 ◽  
Author(s):  
Dilan Qin ◽  
Xiaoxiao He ◽  
Kemin Wang ◽  
Xiaojun Julia Zhao ◽  
Weihong Tan ◽  
...  
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Silica Nanoparticles ◽  
Indirect Immunofluorescence ◽  
Rapid Detection ◽  
Immunofluorescence Microscopy ◽  
Protein A ◽  
Clinical Samples ◽  
Fluorescent Nanoparticles ◽  
Indirect Immunofluorescence Microscopy ◽  
Fluorescent Nanoparticle

A method of fluorescent nanoparticle-based indirect immunofluorescence microscopy (FNP-IIFM) was developed for the rapid detection ofMycobacterium tuberculosis. An anti-Mycobacterium tuberculosisantibody was used as primary antibody to recognizeMycobacterium tuberculosis, and then an antibody binding protein (Protein A) labeled with Tris(2,2-bipyridyl)dichlororuthenium(II) hexahydrate (RuBpy)-doped silica nanoparticles was used to generate fluorescent signal for microscopic examination. Prior to the detection, Protein A was immobilized on RuBpy-doped silica nanoparticles with a coverage of∼5.1×102molecules/nanoparticle. With this method,Mycobacterium tuberculosisin bacterial mixture as well as in spiked sputum was detected. The use of the fluorescent nanoparticles reveals amplified signal intensity and higher photostability than the direct use of conventional fluorescent dye as label. Our preliminary studies have demonstrated the potential application of the FNP-IIFM method for rapid detection ofMycobacterium tuberculosisin clinical samples.

Search mode for rapid detection of virus particles

1991 ◽  
Vol 49 ◽  
pp. 286-287
Author(s):  
O. E. Bradfute
Keyword(s):  
Electron Microscopy ◽  
Rapid Detection ◽  
Plant Virus ◽  
Low Dose ◽  
Search Mode ◽  
Virus Diseases ◽  
Virus Particles ◽  
Focus Image ◽  
Time Required ◽  
Rapid Switching

Electron microscopy is frequently used in preliminary diagnosis of plant virus diseases by surveying negatively stained preparations of crude extracts of leaf samples. A major limitation of this method is the time required to survey grids when the concentration of virus particles (VPs) is low. A rapid survey of grids for VPs is reported here; the method employs a low magnification, out-of-focus Search Mode similar to that used for low dose electron microscopy of radiation sensitive specimens. A higher magnification, in-focus Confirm Mode is used to photograph or confirm the detection of VPs. Setting up the Search Mode by obtaining an out-of-focus image of the specimen in diffraction (K. H. Downing and W. Chiu, private communications) and pre-aligning the image in Search Mode with the image in Confirm Mode facilitates rapid switching between Modes.

Identification of hepatitis a virus in cell cultures by solid phase immune electron microscopy

1986 ◽  
Vol 44 ◽  
pp. 238-239
Author(s):  
C.D. Humphrey ◽  
T.L. Cromeans ◽  
E.H. Cook ◽  
D.W. Bradley
Keyword(s):  
Electron Microscopy ◽  
Liquid Phase ◽  
Cell Cultures ◽  
Rapid Detection ◽  
Hepatitis A ◽  
Hepatitis A Virus ◽  
Solid Phase ◽  
Immune Electron Microscopy ◽  
Detection And Identification

There is a variety of methods available for the rapid detection and identification of viruses by electron microscopy as described in several reviews. The predominant techniques are classified as direct electron microscopy (DEM), immune electron microscopy (IEM), liquid phase immune electron microscopy (LPIEM) and solid phase immune electron microscopy (SPIEM). Each technique has inherent strengths and weaknesses. However, in recent years, the most progress for identifying viruses has been realized by the utilization of SPIEM.

Rapid detection of Rotavirus antigen in stool sample of acute diarrheic children

2012 ◽  
Vol 6 (1) ◽  
pp. 11-13
Author(s):  
Sushmita Roy ◽  
S.M. Shamsuzzaman ◽  
K.Z. Mamun
Keyword(s):  
Stool Sample ◽  
Rapid Detection ◽  
Cross Sectional Study ◽  
Rapid Diagnosis ◽  
Age Group ◽  
College Hospital ◽  
Cross Sectional ◽  
Rotavirus Infection ◽  
Medical College ◽  
Stool Samples

Rotavirus is one of the leading causes of pediatric diarrhea globally. Accurate and rapid diagnosis of Rotavirus diarrhea should reduce unnecessary use of antibiotics and ultimately reduce drug resistance. Study was designed for rapid diagnosis of Rotavirus antigen in stool sample by ICT (Immunochromatographic test) as well as to observe the seasonal variation of rotavirus infection. This cross sectional study was carried out in the department of Microbiology, Dhaka Medical College from January 2011 to December 2011. Eighty stool samples were collected from Dhaka Shishu Hospital and Dhaka Medical College Hospital. All samples were tested for rotavirus antigen by ICT. Among 80 patients, 42 (52.5%) samples were positive for rotavirus antigen. Among these 42 positive samples, 30 (71.43%) were from 0-12 months of age group, 10 (23.81%) from 13 to 24 months of age group and rest 2 (4.76%) from 25 to 36 months of age group. Rotavirus Ag was detected in stool samples from January to April and another peak episode from October to December. Considering the importance of Rotavirus associated diarrhea, rapid detection of Rotavirus infection in human is substantially needed and should be routinely practiced.DOI: http://dx.doi.org/10.3329/bjmm.v6i1.19354 Bangladesh J Med Microbiol 2012; 06(01): 11-13

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